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The new psychoactive substances 25H-NBOMe and 25H-NBOH induce abnormal development in the zebrafish embryo and interact in the DNA major groove

Toxicological effects of 25H-NBOMe and 25H-NBOH recreational drugs on zebrafish embryos and larvae at the end of 96 h exposure period were demonstrated. 25H-NBOH and 25H-NBOMe caused high embryo mortality at 80 and 100 µg mL(−1), respectively. According to the decrease in the concentration tested, l...

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Autores principales: de Barros, Wellington Alves, Nunes, Camila da Silva, Souza, Juliana Alves da Costa Ribeiro, Nascimento, Igor José dos Santos, Figueiredo, Isis Martins, de Aquino, Thiago Mendonça, Vieira, Leonardo, Farias, Davi, Santos, Josué Carinhanha Caldas, de Fátima, Ângelo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8637007/
https://www.ncbi.nlm.nih.gov/pubmed/34888530
http://dx.doi.org/10.1016/j.crtox.2021.11.002
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author de Barros, Wellington Alves
Nunes, Camila da Silva
Souza, Juliana Alves da Costa Ribeiro
Nascimento, Igor José dos Santos
Figueiredo, Isis Martins
de Aquino, Thiago Mendonça
Vieira, Leonardo
Farias, Davi
Santos, Josué Carinhanha Caldas
de Fátima, Ângelo
author_facet de Barros, Wellington Alves
Nunes, Camila da Silva
Souza, Juliana Alves da Costa Ribeiro
Nascimento, Igor José dos Santos
Figueiredo, Isis Martins
de Aquino, Thiago Mendonça
Vieira, Leonardo
Farias, Davi
Santos, Josué Carinhanha Caldas
de Fátima, Ângelo
author_sort de Barros, Wellington Alves
collection PubMed
description Toxicological effects of 25H-NBOMe and 25H-NBOH recreational drugs on zebrafish embryos and larvae at the end of 96 h exposure period were demonstrated. 25H-NBOH and 25H-NBOMe caused high embryo mortality at 80 and 100 µg mL(−1), respectively. According to the decrease in the concentration tested, lethality decreased while non-lethal effects were predominant up to 10 and 50 µg mL(−1) of 25H-NBOH and 25H-NBOMe, respectively, including spine malformation, egg hatching delay, body malformation, otolith malformation, pericardial edema, and blood clotting. We can disclose that these drugs have an affinity for DNA in vitro using biophysical spectroscopic assays and molecular modeling methods. The experiments demonstrated that 25H-NBOH and 25H-NBOMe bind to the unclassical major groove of ctDNA with a binding constant of 27.00 × 10(4) M(−1) and 5.27 × 10(4) M(−1), respectively. Furthermore, these interactions lead to conformational changes in the DNA structure. Therefore, the results observed in the zebrafish embryos and DNA may be correlated.
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spelling pubmed-86370072021-12-08 The new psychoactive substances 25H-NBOMe and 25H-NBOH induce abnormal development in the zebrafish embryo and interact in the DNA major groove de Barros, Wellington Alves Nunes, Camila da Silva Souza, Juliana Alves da Costa Ribeiro Nascimento, Igor José dos Santos Figueiredo, Isis Martins de Aquino, Thiago Mendonça Vieira, Leonardo Farias, Davi Santos, Josué Carinhanha Caldas de Fátima, Ângelo Curr Res Toxicol Article Toxicological effects of 25H-NBOMe and 25H-NBOH recreational drugs on zebrafish embryos and larvae at the end of 96 h exposure period were demonstrated. 25H-NBOH and 25H-NBOMe caused high embryo mortality at 80 and 100 µg mL(−1), respectively. According to the decrease in the concentration tested, lethality decreased while non-lethal effects were predominant up to 10 and 50 µg mL(−1) of 25H-NBOH and 25H-NBOMe, respectively, including spine malformation, egg hatching delay, body malformation, otolith malformation, pericardial edema, and blood clotting. We can disclose that these drugs have an affinity for DNA in vitro using biophysical spectroscopic assays and molecular modeling methods. The experiments demonstrated that 25H-NBOH and 25H-NBOMe bind to the unclassical major groove of ctDNA with a binding constant of 27.00 × 10(4) M(−1) and 5.27 × 10(4) M(−1), respectively. Furthermore, these interactions lead to conformational changes in the DNA structure. Therefore, the results observed in the zebrafish embryos and DNA may be correlated. Elsevier 2021-11-22 /pmc/articles/PMC8637007/ /pubmed/34888530 http://dx.doi.org/10.1016/j.crtox.2021.11.002 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
de Barros, Wellington Alves
Nunes, Camila da Silva
Souza, Juliana Alves da Costa Ribeiro
Nascimento, Igor José dos Santos
Figueiredo, Isis Martins
de Aquino, Thiago Mendonça
Vieira, Leonardo
Farias, Davi
Santos, Josué Carinhanha Caldas
de Fátima, Ângelo
The new psychoactive substances 25H-NBOMe and 25H-NBOH induce abnormal development in the zebrafish embryo and interact in the DNA major groove
title The new psychoactive substances 25H-NBOMe and 25H-NBOH induce abnormal development in the zebrafish embryo and interact in the DNA major groove
title_full The new psychoactive substances 25H-NBOMe and 25H-NBOH induce abnormal development in the zebrafish embryo and interact in the DNA major groove
title_fullStr The new psychoactive substances 25H-NBOMe and 25H-NBOH induce abnormal development in the zebrafish embryo and interact in the DNA major groove
title_full_unstemmed The new psychoactive substances 25H-NBOMe and 25H-NBOH induce abnormal development in the zebrafish embryo and interact in the DNA major groove
title_short The new psychoactive substances 25H-NBOMe and 25H-NBOH induce abnormal development in the zebrafish embryo and interact in the DNA major groove
title_sort new psychoactive substances 25h-nbome and 25h-nboh induce abnormal development in the zebrafish embryo and interact in the dna major groove
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8637007/
https://www.ncbi.nlm.nih.gov/pubmed/34888530
http://dx.doi.org/10.1016/j.crtox.2021.11.002
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