Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue

Here, we present a protocol for collecting large-volume, four-color, single-molecule localization imaging data from neural tissue. We have applied this technique to map the location and identities of chemical synapses across whole cells in mouse retinae. Our sample preparation approach improves 3D S...

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Detalles Bibliográficos
Autores principales: Vatan, Tarlan, Minehart, Jacqueline A., Zhang, Chenghang, Agarwal, Vatsal, Yang, Jerry, Speer, Colenso M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8637648/
https://www.ncbi.nlm.nih.gov/pubmed/34901889
http://dx.doi.org/10.1016/j.xpro.2021.100971

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8637648/
https://www.ncbi.nlm.nih.gov/pubmed/34901889
http://dx.doi.org/10.1016/j.xpro.2021.100971

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