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Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue
Here, we present a protocol for collecting large-volume, four-color, single-molecule localization imaging data from neural tissue. We have applied this technique to map the location and identities of chemical synapses across whole cells in mouse retinae. Our sample preparation approach improves 3D S...
Autores principales: | Vatan, Tarlan, Minehart, Jacqueline A., Zhang, Chenghang, Agarwal, Vatsal, Yang, Jerry, Speer, Colenso M. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8637648/ https://www.ncbi.nlm.nih.gov/pubmed/34901889 http://dx.doi.org/10.1016/j.xpro.2021.100971 |
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