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Construction of miRNA-mRNA network reveals crucial miRNAs and genes in acute myocardial infarction

Acute myocardial infarction (AMI) is a severe cardiovascular disease. This study aimed to identify crucial microRNAs (miRNAs) and mRNAs in AMI by establishing a miRNA-mRNA network. The microarray datasets GSE31568, GSE148153, and GSE66360 were downloaded from the Gene Expression Omnibus (GEO) databa...

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Autores principales: Wang, Kai, Li, Zhongming, Ma, Wenjie, Sun, Yan, Liu, Xianling, Qian, Lijun, Hong, Jian, Lu, Dasheng, Zhang, Jing, Xu, Di
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Editorial Department of Journal of Biomedical Research 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8637659/
https://www.ncbi.nlm.nih.gov/pubmed/34857679
http://dx.doi.org/10.7555/JBR.35.20210088
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author Wang, Kai
Li, Zhongming
Ma, Wenjie
Sun, Yan
Liu, Xianling
Qian, Lijun
Hong, Jian
Lu, Dasheng
Zhang, Jing
Xu, Di
author_facet Wang, Kai
Li, Zhongming
Ma, Wenjie
Sun, Yan
Liu, Xianling
Qian, Lijun
Hong, Jian
Lu, Dasheng
Zhang, Jing
Xu, Di
author_sort Wang, Kai
collection PubMed
description Acute myocardial infarction (AMI) is a severe cardiovascular disease. This study aimed to identify crucial microRNAs (miRNAs) and mRNAs in AMI by establishing a miRNA-mRNA network. The microarray datasets GSE31568, GSE148153, and GSE66360 were downloaded from the Gene Expression Omnibus (GEO) database. We identified differentially expressed miRNAs (DE-miRNAs) and mRNAs (DE-mRNAs) in AMI samples compared with normal control samples. The consistently changing miRNAs in both GSE31568 and GSE148153 datasets were selected as candidate DE-miRNAs. The interactions between the candidate DE-miRNAs and DE-mRNAs were analyzed, and a miRNA-mRNA network and a protein-protein interaction network were constructed, along with functional enrichment and pathway analyses. A total of 209 DE-miRNAs in the GSE31568 dataset, 857 DE-miRNAs in the GSE148153 dataset, and 351 DE-mRNAs in the GSE66360 dataset were identified. Eighteen candidate DE-miRNAs were selected from both the GSE31568 and GSE148153 datasets. Furthermore, miR-646, miR-127-5p, miR-509-5p, miR-509-3-5p, and miR-767-5p were shown to have a higher degree in the miRNA-mRNA network.THBS-1 as well as FOS was a hub gene in the miRNA-mRNA network and the protein-protein interaction (PPI) network, respectively. CDKN1A was important in both miRNA-mRNA network and PPI network. We established a miRNA-mRNA network in AMI and identified five miRNAs and three genes, which might be used as biomarkers and potential therapeutic targets for patients with AMI.
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spelling pubmed-86376592021-12-05 Construction of miRNA-mRNA network reveals crucial miRNAs and genes in acute myocardial infarction Wang, Kai Li, Zhongming Ma, Wenjie Sun, Yan Liu, Xianling Qian, Lijun Hong, Jian Lu, Dasheng Zhang, Jing Xu, Di J Biomed Res Original Article Acute myocardial infarction (AMI) is a severe cardiovascular disease. This study aimed to identify crucial microRNAs (miRNAs) and mRNAs in AMI by establishing a miRNA-mRNA network. The microarray datasets GSE31568, GSE148153, and GSE66360 were downloaded from the Gene Expression Omnibus (GEO) database. We identified differentially expressed miRNAs (DE-miRNAs) and mRNAs (DE-mRNAs) in AMI samples compared with normal control samples. The consistently changing miRNAs in both GSE31568 and GSE148153 datasets were selected as candidate DE-miRNAs. The interactions between the candidate DE-miRNAs and DE-mRNAs were analyzed, and a miRNA-mRNA network and a protein-protein interaction network were constructed, along with functional enrichment and pathway analyses. A total of 209 DE-miRNAs in the GSE31568 dataset, 857 DE-miRNAs in the GSE148153 dataset, and 351 DE-mRNAs in the GSE66360 dataset were identified. Eighteen candidate DE-miRNAs were selected from both the GSE31568 and GSE148153 datasets. Furthermore, miR-646, miR-127-5p, miR-509-5p, miR-509-3-5p, and miR-767-5p were shown to have a higher degree in the miRNA-mRNA network.THBS-1 as well as FOS was a hub gene in the miRNA-mRNA network and the protein-protein interaction (PPI) network, respectively. CDKN1A was important in both miRNA-mRNA network and PPI network. We established a miRNA-mRNA network in AMI and identified five miRNAs and three genes, which might be used as biomarkers and potential therapeutic targets for patients with AMI. Editorial Department of Journal of Biomedical Research 2021-11 2021-10-10 /pmc/articles/PMC8637659/ /pubmed/34857679 http://dx.doi.org/10.7555/JBR.35.20210088 Text en Copyright and License information: Journal of Biomedical Research, CAS Springer-Verlag Berlin Heidelberg 2021 https://creativecommons.org/licenses/by-nc-sa/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/ (https://creativecommons.org/licenses/by-nc-sa/4.0/)
spellingShingle Original Article
Wang, Kai
Li, Zhongming
Ma, Wenjie
Sun, Yan
Liu, Xianling
Qian, Lijun
Hong, Jian
Lu, Dasheng
Zhang, Jing
Xu, Di
Construction of miRNA-mRNA network reveals crucial miRNAs and genes in acute myocardial infarction
title Construction of miRNA-mRNA network reveals crucial miRNAs and genes in acute myocardial infarction
title_full Construction of miRNA-mRNA network reveals crucial miRNAs and genes in acute myocardial infarction
title_fullStr Construction of miRNA-mRNA network reveals crucial miRNAs and genes in acute myocardial infarction
title_full_unstemmed Construction of miRNA-mRNA network reveals crucial miRNAs and genes in acute myocardial infarction
title_short Construction of miRNA-mRNA network reveals crucial miRNAs and genes in acute myocardial infarction
title_sort construction of mirna-mrna network reveals crucial mirnas and genes in acute myocardial infarction
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8637659/
https://www.ncbi.nlm.nih.gov/pubmed/34857679
http://dx.doi.org/10.7555/JBR.35.20210088
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