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Diagnostic value of aquaporin-4-IgG live cell based assay in neuromyelitis optica spectrum disorders
OBJECTIVE: Determine the utility of aquaporin 4 IgG (AQP4-IgG) testing (live cell-based assay) for Neuromyelitis Optica Spectrum Disorders (NMOSD). METHODS: We included Mayo Clinic patients (1/1/2018-12/31/2019) tested for serum AQP4-IgG by live cell-based flow-cytometric assay. Medical records were...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
SAGE Publications
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8637716/ https://www.ncbi.nlm.nih.gov/pubmed/34868626 http://dx.doi.org/10.1177/20552173211052656 |
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| author | Redenbaugh, Vyanka Montalvo, Mayra Sechi, Elia Buciuc, Marina Fryer, James P. McKeon, Andrew Lennon, Vanda A. Mills, John R. Weinshenker, Brian G. Wingerchuk, Dean M. Chen, John J. Tariq Bhatti, M. Lopez Chiriboga, A. Sebastian Pittock, Sean J. Flanagan, Eoin P. |
| author_facet | Redenbaugh, Vyanka Montalvo, Mayra Sechi, Elia Buciuc, Marina Fryer, James P. McKeon, Andrew Lennon, Vanda A. Mills, John R. Weinshenker, Brian G. Wingerchuk, Dean M. Chen, John J. Tariq Bhatti, M. Lopez Chiriboga, A. Sebastian Pittock, Sean J. Flanagan, Eoin P. |
| author_sort | Redenbaugh, Vyanka |
| collection | PubMed |
| description | OBJECTIVE: Determine the utility of aquaporin 4 IgG (AQP4-IgG) testing (live cell-based assay) for Neuromyelitis Optica Spectrum Disorders (NMOSD). METHODS: We included Mayo Clinic patients (1/1/2018-12/31/2019) tested for serum AQP4-IgG by live cell-based flow-cytometric assay. Medical records were reviewed to assess if patients fulfilled 2015 NMOSD criteria. RESULTS: Of 1371 patients tested, 41 were positive (3%) and all fulfilled NMOSD criteria with AQP4-IgG (specificity = 100%). Only 10/1330 testing negative met NMOSD criteria without AQP4-IgG (sensitivity = 80%) and seven of these 10 were MOG-IgG positive. CONCLUSIONS: AQP4-IgG by live cell-based assay was highly specific and without false positives in a high throughput setting. |
| format | Online Article Text |
| id | pubmed-8637716 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | SAGE Publications |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-86377162021-12-03 Diagnostic value of aquaporin-4-IgG live cell based assay in neuromyelitis optica spectrum disorders Redenbaugh, Vyanka Montalvo, Mayra Sechi, Elia Buciuc, Marina Fryer, James P. McKeon, Andrew Lennon, Vanda A. Mills, John R. Weinshenker, Brian G. Wingerchuk, Dean M. Chen, John J. Tariq Bhatti, M. Lopez Chiriboga, A. Sebastian Pittock, Sean J. Flanagan, Eoin P. Mult Scler J Exp Transl Clin Brief Report OBJECTIVE: Determine the utility of aquaporin 4 IgG (AQP4-IgG) testing (live cell-based assay) for Neuromyelitis Optica Spectrum Disorders (NMOSD). METHODS: We included Mayo Clinic patients (1/1/2018-12/31/2019) tested for serum AQP4-IgG by live cell-based flow-cytometric assay. Medical records were reviewed to assess if patients fulfilled 2015 NMOSD criteria. RESULTS: Of 1371 patients tested, 41 were positive (3%) and all fulfilled NMOSD criteria with AQP4-IgG (specificity = 100%). Only 10/1330 testing negative met NMOSD criteria without AQP4-IgG (sensitivity = 80%) and seven of these 10 were MOG-IgG positive. CONCLUSIONS: AQP4-IgG by live cell-based assay was highly specific and without false positives in a high throughput setting. SAGE Publications 2021-11-26 /pmc/articles/PMC8637716/ /pubmed/34868626 http://dx.doi.org/10.1177/20552173211052656 Text en © The Author(s), 2021 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
| spellingShingle | Brief Report Redenbaugh, Vyanka Montalvo, Mayra Sechi, Elia Buciuc, Marina Fryer, James P. McKeon, Andrew Lennon, Vanda A. Mills, John R. Weinshenker, Brian G. Wingerchuk, Dean M. Chen, John J. Tariq Bhatti, M. Lopez Chiriboga, A. Sebastian Pittock, Sean J. Flanagan, Eoin P. Diagnostic value of aquaporin-4-IgG live cell based assay in neuromyelitis optica spectrum disorders |
| title | Diagnostic value of aquaporin-4-IgG live cell based assay in
neuromyelitis optica spectrum disorders |
| title_full | Diagnostic value of aquaporin-4-IgG live cell based assay in
neuromyelitis optica spectrum disorders |
| title_fullStr | Diagnostic value of aquaporin-4-IgG live cell based assay in
neuromyelitis optica spectrum disorders |
| title_full_unstemmed | Diagnostic value of aquaporin-4-IgG live cell based assay in
neuromyelitis optica spectrum disorders |
| title_short | Diagnostic value of aquaporin-4-IgG live cell based assay in
neuromyelitis optica spectrum disorders |
| title_sort | diagnostic value of aquaporin-4-igg live cell based assay in
neuromyelitis optica spectrum disorders |
| topic | Brief Report |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8637716/ https://www.ncbi.nlm.nih.gov/pubmed/34868626 http://dx.doi.org/10.1177/20552173211052656 |
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