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Target identification of mouse stem cell probe CDy1 as ALDH2 and Abcb1b through live-cell affinity-matrix and ABC CRISPRa library
CDy1 is a powerful tool to distingusih embryonic stem cells for reprogramming studies and regeneration medicine. However, the stem cell selectivity mechanism of CDy1 has not been fully understood. Here, we report ALDH2 and ABCB1 as the molecular targets of CDy1, elucidated by live-cell affinity-matr...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
RSC
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8637918/ https://www.ncbi.nlm.nih.gov/pubmed/34977573 http://dx.doi.org/10.1039/d1cb00147g |
| _version_ | 1784608845620838400 |
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| author | Miyamoto, Naoki Go, Young-Hyun Ciaramicoli, Larissa Miasiro Kwon, Haw-Young Kim, Heon Seok Bi, Xuezhi Yu, Young Hyun Kim, Beomsue Ha, Hyung-Ho Kang, Nam-Young Yun, Seong-Wook Kim, Jin-Soo Cha, Hyuk-Jin Chang, Young-Tae |
| author_facet | Miyamoto, Naoki Go, Young-Hyun Ciaramicoli, Larissa Miasiro Kwon, Haw-Young Kim, Heon Seok Bi, Xuezhi Yu, Young Hyun Kim, Beomsue Ha, Hyung-Ho Kang, Nam-Young Yun, Seong-Wook Kim, Jin-Soo Cha, Hyuk-Jin Chang, Young-Tae |
| author_sort | Miyamoto, Naoki |
| collection | PubMed |
| description | CDy1 is a powerful tool to distingusih embryonic stem cells for reprogramming studies and regeneration medicine. However, the stem cell selectivity mechanism of CDy1 has not been fully understood. Here, we report ALDH2 and ABCB1 as the molecular targets of CDy1, elucidated by live-cell affinity-matrix and ABC transporter CRISPRa library screening. The two unique orthogonal mechanisms provide the potential of multi-demensional cellular distinction of specific cell types. |
| format | Online Article Text |
| id | pubmed-8637918 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | RSC |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-86379182021-12-30 Target identification of mouse stem cell probe CDy1 as ALDH2 and Abcb1b through live-cell affinity-matrix and ABC CRISPRa library Miyamoto, Naoki Go, Young-Hyun Ciaramicoli, Larissa Miasiro Kwon, Haw-Young Kim, Heon Seok Bi, Xuezhi Yu, Young Hyun Kim, Beomsue Ha, Hyung-Ho Kang, Nam-Young Yun, Seong-Wook Kim, Jin-Soo Cha, Hyuk-Jin Chang, Young-Tae RSC Chem Biol Chemistry CDy1 is a powerful tool to distingusih embryonic stem cells for reprogramming studies and regeneration medicine. However, the stem cell selectivity mechanism of CDy1 has not been fully understood. Here, we report ALDH2 and ABCB1 as the molecular targets of CDy1, elucidated by live-cell affinity-matrix and ABC transporter CRISPRa library screening. The two unique orthogonal mechanisms provide the potential of multi-demensional cellular distinction of specific cell types. RSC 2021-08-20 /pmc/articles/PMC8637918/ /pubmed/34977573 http://dx.doi.org/10.1039/d1cb00147g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
| spellingShingle | Chemistry Miyamoto, Naoki Go, Young-Hyun Ciaramicoli, Larissa Miasiro Kwon, Haw-Young Kim, Heon Seok Bi, Xuezhi Yu, Young Hyun Kim, Beomsue Ha, Hyung-Ho Kang, Nam-Young Yun, Seong-Wook Kim, Jin-Soo Cha, Hyuk-Jin Chang, Young-Tae Target identification of mouse stem cell probe CDy1 as ALDH2 and Abcb1b through live-cell affinity-matrix and ABC CRISPRa library |
| title | Target identification of mouse stem cell probe CDy1 as ALDH2 and Abcb1b through live-cell affinity-matrix and ABC CRISPRa library |
| title_full | Target identification of mouse stem cell probe CDy1 as ALDH2 and Abcb1b through live-cell affinity-matrix and ABC CRISPRa library |
| title_fullStr | Target identification of mouse stem cell probe CDy1 as ALDH2 and Abcb1b through live-cell affinity-matrix and ABC CRISPRa library |
| title_full_unstemmed | Target identification of mouse stem cell probe CDy1 as ALDH2 and Abcb1b through live-cell affinity-matrix and ABC CRISPRa library |
| title_short | Target identification of mouse stem cell probe CDy1 as ALDH2 and Abcb1b through live-cell affinity-matrix and ABC CRISPRa library |
| title_sort | target identification of mouse stem cell probe cdy1 as aldh2 and abcb1b through live-cell affinity-matrix and abc crispra library |
| topic | Chemistry |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8637918/ https://www.ncbi.nlm.nih.gov/pubmed/34977573 http://dx.doi.org/10.1039/d1cb00147g |
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