Ammonia Fiber Expansion Combined with White Rot Fungi to Treat Lignocellulose for Cultivation of Mushrooms

[Image: see text] In order to improve the degradation efficiency of lignocellulose while increasing the yield of mushrooms, white rot fungi treatment (Pleurotus ostreatus, Pleurotus eryngii, and Pleurotus geesteranus) combined with ammonia fiber expansion was proposed as a method for treating lignocellulose (Pennisetum sinese, salix chips, and pine chips) for mushroom cultivation. Compared with treatment using either ammonia fiber expansion or white rot fungus, the combined treatment significantly improved lignocellulose degradation rate by 10–20% and reduced the time required significantly. Among them, P. geesteranus was the most effective bacterium for the combined treatment of lignocellulose. Ammonia fiber expansion-treated lignocellulose contributed to mycelial growth and increased the activity of three lignin hydrolase enzymes (laccase, manganese peroxidase, and lignin peroxidase) and mushroom yield. The mushroom yield was increased by 44.6%. The combined treatment method proposed in our study improves lignocellulose resource utilization and is therefore useful in the treatment of agricultural solid organic waste.