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The circular RNA expression profile in ovarian serous cystadenocarcinoma reveals a complex circRNA–miRNA regulatory network

BACKGROUND: Ovarian serous cystadenocarcinoma is one of the most serious gynecological malignancies. Circular RNA (circRNA) is a type of noncoding RNA with a covalently closed continuous loop structure. Abnormal circRNA expression might be associated with tumorigenesis because of its complex biologi...

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Autores principales: Zhuang, Minhui, Zhao, Jian, Wu, Jing, Fu, Shilong, Han, Ping, Song, Xiaofeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8638095/
https://www.ncbi.nlm.nih.gov/pubmed/34857007
http://dx.doi.org/10.1186/s12920-021-01132-5
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author Zhuang, Minhui
Zhao, Jian
Wu, Jing
Fu, Shilong
Han, Ping
Song, Xiaofeng
author_facet Zhuang, Minhui
Zhao, Jian
Wu, Jing
Fu, Shilong
Han, Ping
Song, Xiaofeng
author_sort Zhuang, Minhui
collection PubMed
description BACKGROUND: Ovarian serous cystadenocarcinoma is one of the most serious gynecological malignancies. Circular RNA (circRNA) is a type of noncoding RNA with a covalently closed continuous loop structure. Abnormal circRNA expression might be associated with tumorigenesis because of its complex biological mechanisms by, for example, functioning as a microRNA (miRNA) sponge. However, the circRNA expression profile in ovarian serous cystadenocarcinoma and their associations with other RNAs have not yet been characterized. The main purpose of this study was to reveal the circRNA expression profile in ovarian serous cystadenocarcinoma. METHODS: We collected six specimens from three patients with ovarian serous cystadenocarcinoma and adjacent normal tissues. After RNA sequencing, we analyzed the expression of circRNAs with relevant mRNAs and miRNAs to characterize potential function. RESULTS: 15,092 unique circRNAs were identified in six specimens. Approximately 46% of these circRNAs were not recorded in public databases. We then reported 353 differentially expressed circRNAs with oncogenes and tumor-suppressor genes. Furthermore, a conjoint analysis with relevant mRNAs revealed consistent changes between circRNAs and their homologous mRNAs. Overall, construction of a circRNA–miRNA network suggested that 4 special circRNAs could be used as potential biomarkers. CONCLUSIONS: Our study revealed the circRNA expression profile in the tissues of patients with ovarian serous cystadenocarcinoma. The differential expression of circRNAs was thought to be associated with ovarian serous cystadenocarcinoma in the enrichment analysis, and co-expression analysis with relevant mRNAs and miRNAs illustrated the latent regulatory network. We also constructed a complex circRNA–miRNA interaction network and then demonstrated the potential function of certain circRNAs to aid future diagnosis and treatment. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12920-021-01132-5.
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spelling pubmed-86380952021-12-02 The circular RNA expression profile in ovarian serous cystadenocarcinoma reveals a complex circRNA–miRNA regulatory network Zhuang, Minhui Zhao, Jian Wu, Jing Fu, Shilong Han, Ping Song, Xiaofeng BMC Med Genomics Research BACKGROUND: Ovarian serous cystadenocarcinoma is one of the most serious gynecological malignancies. Circular RNA (circRNA) is a type of noncoding RNA with a covalently closed continuous loop structure. Abnormal circRNA expression might be associated with tumorigenesis because of its complex biological mechanisms by, for example, functioning as a microRNA (miRNA) sponge. However, the circRNA expression profile in ovarian serous cystadenocarcinoma and their associations with other RNAs have not yet been characterized. The main purpose of this study was to reveal the circRNA expression profile in ovarian serous cystadenocarcinoma. METHODS: We collected six specimens from three patients with ovarian serous cystadenocarcinoma and adjacent normal tissues. After RNA sequencing, we analyzed the expression of circRNAs with relevant mRNAs and miRNAs to characterize potential function. RESULTS: 15,092 unique circRNAs were identified in six specimens. Approximately 46% of these circRNAs were not recorded in public databases. We then reported 353 differentially expressed circRNAs with oncogenes and tumor-suppressor genes. Furthermore, a conjoint analysis with relevant mRNAs revealed consistent changes between circRNAs and their homologous mRNAs. Overall, construction of a circRNA–miRNA network suggested that 4 special circRNAs could be used as potential biomarkers. CONCLUSIONS: Our study revealed the circRNA expression profile in the tissues of patients with ovarian serous cystadenocarcinoma. The differential expression of circRNAs was thought to be associated with ovarian serous cystadenocarcinoma in the enrichment analysis, and co-expression analysis with relevant mRNAs and miRNAs illustrated the latent regulatory network. We also constructed a complex circRNA–miRNA interaction network and then demonstrated the potential function of certain circRNAs to aid future diagnosis and treatment. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12920-021-01132-5. BioMed Central 2021-12-02 /pmc/articles/PMC8638095/ /pubmed/34857007 http://dx.doi.org/10.1186/s12920-021-01132-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Zhuang, Minhui
Zhao, Jian
Wu, Jing
Fu, Shilong
Han, Ping
Song, Xiaofeng
The circular RNA expression profile in ovarian serous cystadenocarcinoma reveals a complex circRNA–miRNA regulatory network
title The circular RNA expression profile in ovarian serous cystadenocarcinoma reveals a complex circRNA–miRNA regulatory network
title_full The circular RNA expression profile in ovarian serous cystadenocarcinoma reveals a complex circRNA–miRNA regulatory network
title_fullStr The circular RNA expression profile in ovarian serous cystadenocarcinoma reveals a complex circRNA–miRNA regulatory network
title_full_unstemmed The circular RNA expression profile in ovarian serous cystadenocarcinoma reveals a complex circRNA–miRNA regulatory network
title_short The circular RNA expression profile in ovarian serous cystadenocarcinoma reveals a complex circRNA–miRNA regulatory network
title_sort circular rna expression profile in ovarian serous cystadenocarcinoma reveals a complex circrna–mirna regulatory network
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8638095/
https://www.ncbi.nlm.nih.gov/pubmed/34857007
http://dx.doi.org/10.1186/s12920-021-01132-5
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