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Tangnaikang Alleviates Hyperglycemia and Improves Gut Microbiota in Diabetic Mice

Dysregulation of gut microbiota contributes to the development of type 2 diabetes. To investigate the antidiabetic effect of Tangnaikang and its regulation of gut microbiota in diabetic KKAy mice, a type 2 diabetes mouse model was established by feeding KKAy mice with a high-fat diet (HFD) for 2 wee...

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Autores principales: Zhang, Liping, Wang, Fen, He, Hualiang, Jiao, Tingting, Wu, Lili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8639253/
https://www.ncbi.nlm.nih.gov/pubmed/34868327
http://dx.doi.org/10.1155/2021/1089176
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author Zhang, Liping
Wang, Fen
He, Hualiang
Jiao, Tingting
Wu, Lili
author_facet Zhang, Liping
Wang, Fen
He, Hualiang
Jiao, Tingting
Wu, Lili
author_sort Zhang, Liping
collection PubMed
description Dysregulation of gut microbiota contributes to the development of type 2 diabetes. To investigate the antidiabetic effect of Tangnaikang and its regulation of gut microbiota in diabetic KKAy mice, a type 2 diabetes mouse model was established by feeding KKAy mice with a high-fat diet (HFD) for 2 weeks. The diabetic KKAy mice were treated with vehicle, Acarbose, or different doses of Tangnaikang once a day for 8 weeks. The fasting plasma glucose (FPG) levels and bodyweights were measured weekly. The fecal and blood samples were collected 8 weeks after treatment. The 16s rRNA sequencing and bioinformatics analysis were conducted to explore the effects of Tangnaikang treatment on the richness, diversity, and relative abundance of gut microbiota. Compared with other treatments, high-dose Tangnaikang (4.68 g/kg) significantly reduced FPG levels while elevating bodyweights in model mice. Compared with saline treatment, different doses of Tangnaikang significantly increased gut microbial species richness and diversity. Linear discriminant analysis effect size identified potential bacterial biomarkers associated with Tangnaikang treatment. Relative abundance analysis revealed that Tangnaikang treatment modulated the abundance of gut bacteria at the class and genus levels, such as Bacilli, Lactobacillus, and Alistipes. The principal component analysis demonstrated that, compared with the samples of the high-dose group, the samples of medium-dose and low-dose groups were closer to those of the model group. Tangnaikang alleviated hyperglycemia and improved the composition and abundance of gut microbiota in diabetic KKAy mice.
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spelling pubmed-86392532021-12-03 Tangnaikang Alleviates Hyperglycemia and Improves Gut Microbiota in Diabetic Mice Zhang, Liping Wang, Fen He, Hualiang Jiao, Tingting Wu, Lili Evid Based Complement Alternat Med Research Article Dysregulation of gut microbiota contributes to the development of type 2 diabetes. To investigate the antidiabetic effect of Tangnaikang and its regulation of gut microbiota in diabetic KKAy mice, a type 2 diabetes mouse model was established by feeding KKAy mice with a high-fat diet (HFD) for 2 weeks. The diabetic KKAy mice were treated with vehicle, Acarbose, or different doses of Tangnaikang once a day for 8 weeks. The fasting plasma glucose (FPG) levels and bodyweights were measured weekly. The fecal and blood samples were collected 8 weeks after treatment. The 16s rRNA sequencing and bioinformatics analysis were conducted to explore the effects of Tangnaikang treatment on the richness, diversity, and relative abundance of gut microbiota. Compared with other treatments, high-dose Tangnaikang (4.68 g/kg) significantly reduced FPG levels while elevating bodyweights in model mice. Compared with saline treatment, different doses of Tangnaikang significantly increased gut microbial species richness and diversity. Linear discriminant analysis effect size identified potential bacterial biomarkers associated with Tangnaikang treatment. Relative abundance analysis revealed that Tangnaikang treatment modulated the abundance of gut bacteria at the class and genus levels, such as Bacilli, Lactobacillus, and Alistipes. The principal component analysis demonstrated that, compared with the samples of the high-dose group, the samples of medium-dose and low-dose groups were closer to those of the model group. Tangnaikang alleviated hyperglycemia and improved the composition and abundance of gut microbiota in diabetic KKAy mice. Hindawi 2021-11-25 /pmc/articles/PMC8639253/ /pubmed/34868327 http://dx.doi.org/10.1155/2021/1089176 Text en Copyright © 2021 Liping Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Liping
Wang, Fen
He, Hualiang
Jiao, Tingting
Wu, Lili
Tangnaikang Alleviates Hyperglycemia and Improves Gut Microbiota in Diabetic Mice
title Tangnaikang Alleviates Hyperglycemia and Improves Gut Microbiota in Diabetic Mice
title_full Tangnaikang Alleviates Hyperglycemia and Improves Gut Microbiota in Diabetic Mice
title_fullStr Tangnaikang Alleviates Hyperglycemia and Improves Gut Microbiota in Diabetic Mice
title_full_unstemmed Tangnaikang Alleviates Hyperglycemia and Improves Gut Microbiota in Diabetic Mice
title_short Tangnaikang Alleviates Hyperglycemia and Improves Gut Microbiota in Diabetic Mice
title_sort tangnaikang alleviates hyperglycemia and improves gut microbiota in diabetic mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8639253/
https://www.ncbi.nlm.nih.gov/pubmed/34868327
http://dx.doi.org/10.1155/2021/1089176
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