Global metabolome profiling of COVID-19 respiratory specimen using high-resolution mass spectrometry (HRMS)

Here we describe a protocol for identifying metabolites in respiratory specimens of patients that are SARS-CoV-2 positive, SARS-CoV-2 negative, or H1N1 positive. This protocol provides step-by-step instructions on sample collection from patients, followed by metabolite extraction. We use ultra-high-...

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Detalles Bibliográficos
Autores principales: Sharma, Nupur, Bhat, Sadam H., Tripathi, Gaurav, Yadav, Manisha, Mathew, Babu, Bindal, Vasundhra, Sharma, Shvetank, Gupta, Ekta, Maras, Jaswinder Singh, Sarin, Shiv Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8639449/
https://www.ncbi.nlm.nih.gov/pubmed/34877545
http://dx.doi.org/10.1016/j.xpro.2021.101051
Descripción
Sumario:Here we describe a protocol for identifying metabolites in respiratory specimens of patients that are SARS-CoV-2 positive, SARS-CoV-2 negative, or H1N1 positive. This protocol provides step-by-step instructions on sample collection from patients, followed by metabolite extraction. We use ultra-high-pressure liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) for data acquisition and describe the steps for data analysis. The protocol was standardized with specific customization for SARS-CoV-2-containing respiratory specimens. For complete details on the use and execution of this protocol, please refer to Maras et al. (2021).

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