Global metabolome profiling of COVID-19 respiratory specimen using high-resolution mass spectrometry (HRMS)

Here we describe a protocol for identifying metabolites in respiratory specimens of patients that are SARS-CoV-2 positive, SARS-CoV-2 negative, or H1N1 positive. This protocol provides step-by-step instructions on sample collection from patients, followed by metabolite extraction. We use ultra-high-...

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Detalles Bibliográficos
Autores principales: Sharma, Nupur, Bhat, Sadam H., Tripathi, Gaurav, Yadav, Manisha, Mathew, Babu, Bindal, Vasundhra, Sharma, Shvetank, Gupta, Ekta, Maras, Jaswinder Singh, Sarin, Shiv Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8639449/
https://www.ncbi.nlm.nih.gov/pubmed/34877545
http://dx.doi.org/10.1016/j.xpro.2021.101051
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author Sharma, Nupur
Bhat, Sadam H.
Tripathi, Gaurav
Yadav, Manisha
Mathew, Babu
Bindal, Vasundhra
Sharma, Shvetank
Gupta, Ekta
Maras, Jaswinder Singh
Sarin, Shiv Kumar
author_facet Sharma, Nupur
Bhat, Sadam H.
Tripathi, Gaurav
Yadav, Manisha
Mathew, Babu
Bindal, Vasundhra
Sharma, Shvetank
Gupta, Ekta
Maras, Jaswinder Singh
Sarin, Shiv Kumar
author_sort Sharma, Nupur
collection PubMed
description Here we describe a protocol for identifying metabolites in respiratory specimens of patients that are SARS-CoV-2 positive, SARS-CoV-2 negative, or H1N1 positive. This protocol provides step-by-step instructions on sample collection from patients, followed by metabolite extraction. We use ultra-high-pressure liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) for data acquisition and describe the steps for data analysis. The protocol was standardized with specific customization for SARS-CoV-2-containing respiratory specimens. For complete details on the use and execution of this protocol, please refer to Maras et al. (2021).
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spelling pubmed-86394492021-12-03 Global metabolome profiling of COVID-19 respiratory specimen using high-resolution mass spectrometry (HRMS) Sharma, Nupur Bhat, Sadam H. Tripathi, Gaurav Yadav, Manisha Mathew, Babu Bindal, Vasundhra Sharma, Shvetank Gupta, Ekta Maras, Jaswinder Singh Sarin, Shiv Kumar STAR Protoc Protocol Here we describe a protocol for identifying metabolites in respiratory specimens of patients that are SARS-CoV-2 positive, SARS-CoV-2 negative, or H1N1 positive. This protocol provides step-by-step instructions on sample collection from patients, followed by metabolite extraction. We use ultra-high-pressure liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) for data acquisition and describe the steps for data analysis. The protocol was standardized with specific customization for SARS-CoV-2-containing respiratory specimens. For complete details on the use and execution of this protocol, please refer to Maras et al. (2021). Elsevier 2021-12-03 /pmc/articles/PMC8639449/ /pubmed/34877545 http://dx.doi.org/10.1016/j.xpro.2021.101051 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Sharma, Nupur
Bhat, Sadam H.
Tripathi, Gaurav
Yadav, Manisha
Mathew, Babu
Bindal, Vasundhra
Sharma, Shvetank
Gupta, Ekta
Maras, Jaswinder Singh
Sarin, Shiv Kumar
Global metabolome profiling of COVID-19 respiratory specimen using high-resolution mass spectrometry (HRMS)
title Global metabolome profiling of COVID-19 respiratory specimen using high-resolution mass spectrometry (HRMS)
title_full Global metabolome profiling of COVID-19 respiratory specimen using high-resolution mass spectrometry (HRMS)
title_fullStr Global metabolome profiling of COVID-19 respiratory specimen using high-resolution mass spectrometry (HRMS)
title_full_unstemmed Global metabolome profiling of COVID-19 respiratory specimen using high-resolution mass spectrometry (HRMS)
title_short Global metabolome profiling of COVID-19 respiratory specimen using high-resolution mass spectrometry (HRMS)
title_sort global metabolome profiling of covid-19 respiratory specimen using high-resolution mass spectrometry (hrms)
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8639449/
https://www.ncbi.nlm.nih.gov/pubmed/34877545
http://dx.doi.org/10.1016/j.xpro.2021.101051
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