Cargando…

Global metabolome profiling of COVID-19 respiratory specimen using high-resolution mass spectrometry (HRMS)

Here we describe a protocol for identifying metabolites in respiratory specimens of patients that are SARS-CoV-2 positive, SARS-CoV-2 negative, or H1N1 positive. This protocol provides step-by-step instructions on sample collection from patients, followed by metabolite extraction. We use ultra-high-...

Descripción completa

Detalles Bibliográficos
Autores principales:	Sharma, Nupur, Bhat, Sadam H., Tripathi, Gaurav, Yadav, Manisha, Mathew, Babu, Bindal, Vasundhra, Sharma, Shvetank, Gupta, Ekta, Maras, Jaswinder Singh, Sarin, Shiv Kumar
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8639449/ https://www.ncbi.nlm.nih.gov/pubmed/34877545 http://dx.doi.org/10.1016/j.xpro.2021.101051

Ejemplares similares

Protocol for global proteome, virome, and metaproteome profiling of respiratory specimen (VTM) in COVID-19 patient by LC-MS/MS-based analysis
por: Tripathi, Gaurav, et al.
Publicado: (2021)

Baseline Plasma Metabotype Correlates With Direct-Acting Antiviral Therapy Nonresponse for HCV in HIV–HCV Coinfected Patients
por: Tripathi, Gaurav, et al.
Publicado: (2022)

Multi-omics analysis of respiratory specimen characterizes baseline molecular determinants associated with SARS-CoV-2 outcome
por: Maras, Jaswinder Singh, et al.
Publicado: (2021)

Differential blood transcriptome modules predict response to corticosteroid therapy in alcoholic hepatitis
por: Sharma, Shvetank, et al.
Publicado: (2021)

Baseline urine metabolic phenotype in patients with severe alcoholic hepatitis and its association with outcome
por: Maras, Jaswinder Singh, et al.
Publicado: (2018)

Iron-Overload triggers ADAM-17 mediated inflammation in Severe Alcoholic Hepatitis
por: Maras, Jaswinder Singh, et al.
Publicado: (2018)

Predictors of steroid non-response and new approaches in severe alcoholic hepatitis
por: Sarin, Shiv Kumar, et al.
Publicado: (2020)

Ocimum sanctum Alters the Lipid Landscape of the Brain Cortex and Plasma to Ameliorate the Effect of Photothrombotic Stroke in a Mouse Model
por: Yadav, Inderjeet, et al.
Publicado: (2023)

Hyperoxidized Albumin Modulates Platelets and Promotes Inflammation Through CD36 Receptor in Severe Alcoholic Hepatitis
por: Bhat, Adil, et al.
Publicado: (2019)

Dysregulated Lipid Transport Proteins Correlate With Pathogenesis and Outcome in Severe Alcoholic Hepatitis
por: Maras, Jaswinder Singh, et al.
Publicado: (2019)

Pre-therapy liver transcriptome landscape in Indian and French patients with severe alcoholic hepatitis and steroid responsiveness
por: Sharma, Shvetank, et al.
Publicado: (2017)

SIRT2 inhibition by AGK2 enhances mycobacteria-specific stem cell memory responses by modulating beta-catenin and glycolysis
por: Bhaskar, Ashima, et al.
Publicado: (2023)

Maternal Immunity Influences Vertical Transmission of Hepatitis B to Newborns
por: Vyas, Ashish Kumar, et al.
Publicado: (2019)

Elevated plasma ICAM1 levels predict 28-day mortality in cirrhotic patients with COVID-19 or bacterial sepsis
por: Kaur, Savneet, et al.
Publicado: (2021)

Comprehensive metabolomic profiling of osteosarcoma based on UHPLC-HRMS
por: Lv, Dongming, et al.
Publicado: (2020)

Increased Expression of RUNX1 in Liver Correlates with NASH Activity Score in Patients with Non-Alcoholic Steatohepatitis (NASH)
por: Kaur, Savneet, et al.
Publicado: (2019)

An automatic UPLC‐HRMS data analysis platform for plant metabolomics
por: Liu, Pingping, et al.
Publicado: (2019)

Study of the Royal Jelly Free Fatty Acids by Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS)
por: Kokotou, Maroula G., et al.
Publicado: (2020)

Identification of Flavonoids in the Leaves of Eranthis longistipitata (Ranunculaceae) by Liquid Chromatography with High-Resolution Mass Spectrometry (LC-HRMS)
por: Kostikova, Vera A., et al.
Publicado: (2021)

Improved Survival with the Patients with Variceal Bleed
por: Sharma, Praveen, et al.
Publicado: (2011)

Lower Rates Of Naturally Occurring Resistance-Associated Substitutions (RASs) In Hepatitis C Virus (HCV)-Infected Chronic Kidney Disease (CKD) Patients Than In HCV-Infected Patients With Only Liver Disease
por: Gupta, Ekta, et al.
Publicado: (2019)

Untargeted Ultrahigh-Performance Liquid Chromatography-Hybrid Quadrupole-Orbitrap Mass Spectrometry (UHPLC-HRMS) Metabolomics Reveals Propolis Markers of Greek and Chinese Origin
por: Stavropoulou, Maria-Ioanna, et al.
Publicado: (2021)

Ammonium Fluoride as Suitable Additive for HILIC-Based LC-HRMS Metabolomics
por: Narduzzi, Luca, et al.
Publicado: (2019)

Biomarker discovery in galactosemia: Metabolomics with UPLC/HRMS in dried blood spots
por: Alodaib, Ahmad N., et al.
Publicado: (2023)

Diagnostic dilemmas in human immunodeficiency virus testing
por: Choudhary, Aashish, et al.
Publicado: (2014)

LC–HRMS determination of piperine on rat dried blood spots: A pharmacokinetic study()
por: Ramesh, Bokka, et al.
Publicado: (2016)

Geographical Origin Discrimination of Monofloral Honeys by Direct Analysis in Real Time Ionization-High Resolution Mass Spectrometry (DART-HRMS)
por: Lippolis, Vincenzo, et al.
Publicado: (2020)

Liquid Chromatography–High-Resolution Mass Spectrometry (LC-HRMS) Profiling of Commercial Enocianina and Evaluation of Their Antioxidant and Anti-Inflammatory Activity
por: Della Vedova, Larissa, et al.
Publicado: (2022)

Authentication of Edible Insects’ Powders by the Combination of DART-HRMS Signatures: The First Application of Ambient Mass Spectrometry to Screening of Novel Food
por: Tata, Alessandra, et al.
Publicado: (2022)

Berberine governs NOTCH3/AKT signaling to enrich lung-resident memory T cells during tuberculosis
por: Pahuja, Isha, et al.
Publicado: (2023)

UHPLC-HRMS/MS on untargeted metabolomics: a case study with Copaifera (Fabaceae)
por: Antonio, Ananda da Silva, et al.
Publicado: (2021)

Towards Unbiased Evaluation of Ionization Performance in LC-HRMS Metabolomics Method Development
por: Jaeger, Carsten, et al.
Publicado: (2022)

UHPLC-HRMS-based metabolomic and lipidomic characterization of glioma cells in response to anlotinib
por: Shi, Yingying, et al.
Publicado: (2023)

Detection of Mycotoxins in Highly Matrix-Loaded House-Dust Samples by QTOF-HRMS, IM-QTOF-HRMS, and TQMS: Advantages and Disadvantages
por: Lindemann, Viktoria, et al.
Publicado: (2022)

Differential Effects of mTORC1 and mTORC2 Inhibition on High-Resolution Mass Spectrometry (HRMS) Metabolomics and the Internal Exposome in Pancreatic Beta Cell Lines
por: Soliman, Ghada, et al.
Publicado: (2022)

Optimizing Secondary Electrospray Ionization High-Resolution Mass Spectrometry (SESI-HRMS) for the Analysis of Volatile Fatty Acids from Gut Microbiome
por: Lee, Jisun H. J., et al.
Publicado: (2020)

Differential Analysis of Serum Principal Components Treated with Compound Sophora Decoction and Related Compounds Based on High-Resolution Mass Spectrometry (HRMS)
por: Sun, Wanjin, et al.
Publicado: (2020)

Development, Validation, and Comparison of Two Mass Spectrometry Methods (LC-MS/HRMS and LC-MS/MS) for the Quantification of Rituximab in Human Plasma
por: Millet, Aurélien, et al.
Publicado: (2021)

Suboptimal Level of Bone‐Forming Cells in Advanced Cirrhosis are Associated with Hepatic Osteodystrophy
por: Bihari, Chhagan, et al.
Publicado: (2018)

Evaluation of impact of occult hepatitis B infection in chronic HCV-infected patients: A retrospective cohort study
por: Bhatia, Mohit, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_2efrgu7tsj6aavnhe657lk37p6