Cargando…

MiR-29b-1-5p regulates the proliferation and differentiation of chicken primary myoblasts and analysis of its effective targets

Several recent studies investigated the role of the miR-29 family in muscle development. However, only a few studies focused on chicken skeletal muscle. In the present study, cell cycle, 5-ethynyl-2’-deoxyuridine (EdU), cell counting kit-8 (CCK-8), and other assays indicated that miR-29b-1-5p can in...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Yuanfang, Zhai, Bin, Yuan, Pengtao, Fan, Shengxin, Jin, Wenjiao, Li, Wenting, Sun, Guirong, Tian, Yadong, Liu, Xiaojun, Kang, Xiangtao, Li, Guoxi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8639469/
https://www.ncbi.nlm.nih.gov/pubmed/34852967
http://dx.doi.org/10.1016/j.psj.2021.101557
_version_ 1784609153879113728
author Li, Yuanfang
Zhai, Bin
Yuan, Pengtao
Fan, Shengxin
Jin, Wenjiao
Li, Wenting
Sun, Guirong
Tian, Yadong
Liu, Xiaojun
Kang, Xiangtao
Li, Guoxi
author_facet Li, Yuanfang
Zhai, Bin
Yuan, Pengtao
Fan, Shengxin
Jin, Wenjiao
Li, Wenting
Sun, Guirong
Tian, Yadong
Liu, Xiaojun
Kang, Xiangtao
Li, Guoxi
author_sort Li, Yuanfang
collection PubMed
description Several recent studies investigated the role of the miR-29 family in muscle development. However, only a few studies focused on chicken skeletal muscle. In the present study, cell cycle, 5-ethynyl-2’-deoxyuridine (EdU), cell counting kit-8 (CCK-8), and other assays indicated that miR-29b-1-5p can inhibit the proliferation of chicken primary myoblasts (CPMs); the western blot assay and immunofluorescence detection of MYHC demonstrated that miR-29b-1-5p can promote the differentiation of myoblasts. The functional enrichment analysis revealed that the target genes of miR-29b-1-5p may be involved in muscle tissue development, muscle organ development, and striated muscle tissue development, which are biological processes related to muscle development. The correlation analysis showed that these 6 genes, that is, ankyrin repeat domain 9 (ANKRD9), lactate dehydrogenase A (LDHA), transcription factor 12 (TCF12), FAT atypical cadherin 1 (FAT1), lin-9 homolog (LIN9), and integrin beta 3 binding protein (ITGB3BP), can be used as effective candidate target genes of miR-29b-1-5p. Moreover, miR-29b-1-5p inhibits the expression of ANKRD9 by directly binding the 3′UTR of ANKRD9. Overall, these data indicate that miR-29b-1-5p inhibits the proliferation of primary chicken myoblasts, stimulates their differentiation, and is involved in the process of muscle development and that its effective target gene is ANKRD9. This study identified the molecular mechanism of miR-29b-1-5p in chicken muscle development.
format Online
Article
Text
id pubmed-8639469
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-86394692021-12-09 MiR-29b-1-5p regulates the proliferation and differentiation of chicken primary myoblasts and analysis of its effective targets Li, Yuanfang Zhai, Bin Yuan, Pengtao Fan, Shengxin Jin, Wenjiao Li, Wenting Sun, Guirong Tian, Yadong Liu, Xiaojun Kang, Xiangtao Li, Guoxi Poult Sci GENETICS AND MOLECULAR BIOLOGY Several recent studies investigated the role of the miR-29 family in muscle development. However, only a few studies focused on chicken skeletal muscle. In the present study, cell cycle, 5-ethynyl-2’-deoxyuridine (EdU), cell counting kit-8 (CCK-8), and other assays indicated that miR-29b-1-5p can inhibit the proliferation of chicken primary myoblasts (CPMs); the western blot assay and immunofluorescence detection of MYHC demonstrated that miR-29b-1-5p can promote the differentiation of myoblasts. The functional enrichment analysis revealed that the target genes of miR-29b-1-5p may be involved in muscle tissue development, muscle organ development, and striated muscle tissue development, which are biological processes related to muscle development. The correlation analysis showed that these 6 genes, that is, ankyrin repeat domain 9 (ANKRD9), lactate dehydrogenase A (LDHA), transcription factor 12 (TCF12), FAT atypical cadherin 1 (FAT1), lin-9 homolog (LIN9), and integrin beta 3 binding protein (ITGB3BP), can be used as effective candidate target genes of miR-29b-1-5p. Moreover, miR-29b-1-5p inhibits the expression of ANKRD9 by directly binding the 3′UTR of ANKRD9. Overall, these data indicate that miR-29b-1-5p inhibits the proliferation of primary chicken myoblasts, stimulates their differentiation, and is involved in the process of muscle development and that its effective target gene is ANKRD9. This study identified the molecular mechanism of miR-29b-1-5p in chicken muscle development. Elsevier 2021-11-02 /pmc/articles/PMC8639469/ /pubmed/34852967 http://dx.doi.org/10.1016/j.psj.2021.101557 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle GENETICS AND MOLECULAR BIOLOGY
Li, Yuanfang
Zhai, Bin
Yuan, Pengtao
Fan, Shengxin
Jin, Wenjiao
Li, Wenting
Sun, Guirong
Tian, Yadong
Liu, Xiaojun
Kang, Xiangtao
Li, Guoxi
MiR-29b-1-5p regulates the proliferation and differentiation of chicken primary myoblasts and analysis of its effective targets
title MiR-29b-1-5p regulates the proliferation and differentiation of chicken primary myoblasts and analysis of its effective targets
title_full MiR-29b-1-5p regulates the proliferation and differentiation of chicken primary myoblasts and analysis of its effective targets
title_fullStr MiR-29b-1-5p regulates the proliferation and differentiation of chicken primary myoblasts and analysis of its effective targets
title_full_unstemmed MiR-29b-1-5p regulates the proliferation and differentiation of chicken primary myoblasts and analysis of its effective targets
title_short MiR-29b-1-5p regulates the proliferation and differentiation of chicken primary myoblasts and analysis of its effective targets
title_sort mir-29b-1-5p regulates the proliferation and differentiation of chicken primary myoblasts and analysis of its effective targets
topic GENETICS AND MOLECULAR BIOLOGY
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8639469/
https://www.ncbi.nlm.nih.gov/pubmed/34852967
http://dx.doi.org/10.1016/j.psj.2021.101557
work_keys_str_mv AT liyuanfang mir29b15pregulatestheproliferationanddifferentiationofchickenprimarymyoblastsandanalysisofitseffectivetargets
AT zhaibin mir29b15pregulatestheproliferationanddifferentiationofchickenprimarymyoblastsandanalysisofitseffectivetargets
AT yuanpengtao mir29b15pregulatestheproliferationanddifferentiationofchickenprimarymyoblastsandanalysisofitseffectivetargets
AT fanshengxin mir29b15pregulatestheproliferationanddifferentiationofchickenprimarymyoblastsandanalysisofitseffectivetargets
AT jinwenjiao mir29b15pregulatestheproliferationanddifferentiationofchickenprimarymyoblastsandanalysisofitseffectivetargets
AT liwenting mir29b15pregulatestheproliferationanddifferentiationofchickenprimarymyoblastsandanalysisofitseffectivetargets
AT sunguirong mir29b15pregulatestheproliferationanddifferentiationofchickenprimarymyoblastsandanalysisofitseffectivetargets
AT tianyadong mir29b15pregulatestheproliferationanddifferentiationofchickenprimarymyoblastsandanalysisofitseffectivetargets
AT liuxiaojun mir29b15pregulatestheproliferationanddifferentiationofchickenprimarymyoblastsandanalysisofitseffectivetargets
AT kangxiangtao mir29b15pregulatestheproliferationanddifferentiationofchickenprimarymyoblastsandanalysisofitseffectivetargets
AT liguoxi mir29b15pregulatestheproliferationanddifferentiationofchickenprimarymyoblastsandanalysisofitseffectivetargets