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In situ EPR spectroscopy of a bacterial membrane transporter using an expanded genetic code

The membrane transporter BtuB is site-directedly spin labelled on the surface of living Escherichia coli via Diels–Alder click chemistry of the genetically encoded amino acid SCO-l-lysine. The previously introduced photoactivatable nitroxide PaNDA prevents off-target labelling, is used for distance...

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Detalles Bibliográficos
Autores principales:	Kugele, Anandi, Ketter, Sophie, Silkenath, Bjarne, Wittmann, Valentin, Joseph, Benesh, Drescher, Malte
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Royal Society of Chemistry 2021
Materias:	Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8640571/ https://www.ncbi.nlm.nih.gov/pubmed/34792069 http://dx.doi.org/10.1039/d1cc04612h

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