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Mass Spectrometry Imaging of Arabidopsis thaliana Leaves at the Single-Cell Level by Infrared Laser Ablation Atmospheric Pressure Photoionization (LAAPPI)

[Image: see text] In this study, we show that infrared laser ablation atmospheric pressure photoionization mass spectrometry (LAAPPI-MS) imaging with 70 μm lateral resolution allows for the analysis of Arabidopsis thaliana (A. thaliana) leaf substructures ranging from single-cell trichomes and the i...

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Autores principales: Hieta, Juha-Pekka, Sipari, Nina, Räikkönen, Heikki, Keinänen, Markku, Kostiainen, Risto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8640987/
https://www.ncbi.nlm.nih.gov/pubmed/34738804
http://dx.doi.org/10.1021/jasms.1c00295
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author Hieta, Juha-Pekka
Sipari, Nina
Räikkönen, Heikki
Keinänen, Markku
Kostiainen, Risto
author_facet Hieta, Juha-Pekka
Sipari, Nina
Räikkönen, Heikki
Keinänen, Markku
Kostiainen, Risto
author_sort Hieta, Juha-Pekka
collection PubMed
description [Image: see text] In this study, we show that infrared laser ablation atmospheric pressure photoionization mass spectrometry (LAAPPI-MS) imaging with 70 μm lateral resolution allows for the analysis of Arabidopsis thaliana (A. thaliana) leaf substructures ranging from single-cell trichomes and the interveinal leaf lamina to primary, secondary, and tertiary veins. The method also showed its potential for depth profiling analysis for the first time by mapping analytes at the different depths of the leaf and spatially resolving the topmost trichomes and cuticular wax layer from the underlying tissues. Negative ion LAAPPI-MS detected many different flavonol glycosides, fatty acids, fatty acid esters, galactolipids, and glycosphingolipids, whose distributions varied significantly between the different substructures of A. thaliana leaves. The results show that LAAPPI-MS provides a highly promising new tool to study the role of metabolites in plants.
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spelling pubmed-86409872021-12-03 Mass Spectrometry Imaging of Arabidopsis thaliana Leaves at the Single-Cell Level by Infrared Laser Ablation Atmospheric Pressure Photoionization (LAAPPI) Hieta, Juha-Pekka Sipari, Nina Räikkönen, Heikki Keinänen, Markku Kostiainen, Risto J Am Soc Mass Spectrom [Image: see text] In this study, we show that infrared laser ablation atmospheric pressure photoionization mass spectrometry (LAAPPI-MS) imaging with 70 μm lateral resolution allows for the analysis of Arabidopsis thaliana (A. thaliana) leaf substructures ranging from single-cell trichomes and the interveinal leaf lamina to primary, secondary, and tertiary veins. The method also showed its potential for depth profiling analysis for the first time by mapping analytes at the different depths of the leaf and spatially resolving the topmost trichomes and cuticular wax layer from the underlying tissues. Negative ion LAAPPI-MS detected many different flavonol glycosides, fatty acids, fatty acid esters, galactolipids, and glycosphingolipids, whose distributions varied significantly between the different substructures of A. thaliana leaves. The results show that LAAPPI-MS provides a highly promising new tool to study the role of metabolites in plants. American Chemical Society 2021-11-05 2021-12-01 /pmc/articles/PMC8640987/ /pubmed/34738804 http://dx.doi.org/10.1021/jasms.1c00295 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Hieta, Juha-Pekka
Sipari, Nina
Räikkönen, Heikki
Keinänen, Markku
Kostiainen, Risto
Mass Spectrometry Imaging of Arabidopsis thaliana Leaves at the Single-Cell Level by Infrared Laser Ablation Atmospheric Pressure Photoionization (LAAPPI)
title Mass Spectrometry Imaging of Arabidopsis thaliana Leaves at the Single-Cell Level by Infrared Laser Ablation Atmospheric Pressure Photoionization (LAAPPI)
title_full Mass Spectrometry Imaging of Arabidopsis thaliana Leaves at the Single-Cell Level by Infrared Laser Ablation Atmospheric Pressure Photoionization (LAAPPI)
title_fullStr Mass Spectrometry Imaging of Arabidopsis thaliana Leaves at the Single-Cell Level by Infrared Laser Ablation Atmospheric Pressure Photoionization (LAAPPI)
title_full_unstemmed Mass Spectrometry Imaging of Arabidopsis thaliana Leaves at the Single-Cell Level by Infrared Laser Ablation Atmospheric Pressure Photoionization (LAAPPI)
title_short Mass Spectrometry Imaging of Arabidopsis thaliana Leaves at the Single-Cell Level by Infrared Laser Ablation Atmospheric Pressure Photoionization (LAAPPI)
title_sort mass spectrometry imaging of arabidopsis thaliana leaves at the single-cell level by infrared laser ablation atmospheric pressure photoionization (laappi)
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8640987/
https://www.ncbi.nlm.nih.gov/pubmed/34738804
http://dx.doi.org/10.1021/jasms.1c00295
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