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Nonrandom Selection and Multiple Blood Feeding of Human Hosts by Anopheles Vectors: Implications for Malaria Transmission in Papua New Guinea

Nonrandom selection and multiple blood feeding of human hosts by Anopheles mosquitoes may exacerbate malaria transmission. Both patterns of blood feeding and their relationship to malaria epidemiology were investigated in Anopheles vectors in Papua New Guinea (PNG). Blood samples from humans and mos...

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Autores principales: Keven, John B., Katusele, Michelle, Vinit, Rebecca, Rodríguez-Rodríguez, Daniela, Hetzel, Manuel W., Robinson, Leanne J., Laman, Moses, Karl, Stephan, Foran, David R., Walker, Edward D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society of Tropical Medicine and Hygiene 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8641310/
https://www.ncbi.nlm.nih.gov/pubmed/34583342
http://dx.doi.org/10.4269/ajtmh.21-0210
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author Keven, John B.
Katusele, Michelle
Vinit, Rebecca
Rodríguez-Rodríguez, Daniela
Hetzel, Manuel W.
Robinson, Leanne J.
Laman, Moses
Karl, Stephan
Foran, David R.
Walker, Edward D.
author_facet Keven, John B.
Katusele, Michelle
Vinit, Rebecca
Rodríguez-Rodríguez, Daniela
Hetzel, Manuel W.
Robinson, Leanne J.
Laman, Moses
Karl, Stephan
Foran, David R.
Walker, Edward D.
author_sort Keven, John B.
collection PubMed
description Nonrandom selection and multiple blood feeding of human hosts by Anopheles mosquitoes may exacerbate malaria transmission. Both patterns of blood feeding and their relationship to malaria epidemiology were investigated in Anopheles vectors in Papua New Guinea (PNG). Blood samples from humans and mosquito blood meals were collected in villages and human genetic profiles (“fingerprints”) were analyzed by genotyping 23 microsatellites and a sex-specific marker. Frequency of blood meals acquired from different humans, identified by unique genetic profiles, was fitted to Poisson and negative binomial distributions to test for nonrandom patterns of host selection. Blood meals with more than one genetic profiles were classified as mosquitoes that fed on multiple humans. The age of a person bitten by a mosquito was determined by matching the blood-meal genetic profile to the villagers’ genetic profiles. Malaria infection in humans was determined by PCR test of blood samples. The results show nonrandom distribution of blood feeding among humans, with biased selection toward males and individuals aged 15–30 years. Prevalence of Plasmodium falciparum infection was higher in this age group, suggesting males in this age range could be super-spreaders of malaria parasites. The proportion of mosquitoes that fed on multiple humans ranged from 6% to 13% among villages. The patterns of host utilization observed here can amplify transmission and contribute to the persistence of malaria in PNG despite efforts to suppress it with insecticidal bed nets. Excessive feeding on males aged 15–30 years underscores the importance of targeted interventions focusing on this demographic group.
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spelling pubmed-86413102021-12-10 Nonrandom Selection and Multiple Blood Feeding of Human Hosts by Anopheles Vectors: Implications for Malaria Transmission in Papua New Guinea Keven, John B. Katusele, Michelle Vinit, Rebecca Rodríguez-Rodríguez, Daniela Hetzel, Manuel W. Robinson, Leanne J. Laman, Moses Karl, Stephan Foran, David R. Walker, Edward D. Am J Trop Med Hyg Articles Nonrandom selection and multiple blood feeding of human hosts by Anopheles mosquitoes may exacerbate malaria transmission. Both patterns of blood feeding and their relationship to malaria epidemiology were investigated in Anopheles vectors in Papua New Guinea (PNG). Blood samples from humans and mosquito blood meals were collected in villages and human genetic profiles (“fingerprints”) were analyzed by genotyping 23 microsatellites and a sex-specific marker. Frequency of blood meals acquired from different humans, identified by unique genetic profiles, was fitted to Poisson and negative binomial distributions to test for nonrandom patterns of host selection. Blood meals with more than one genetic profiles were classified as mosquitoes that fed on multiple humans. The age of a person bitten by a mosquito was determined by matching the blood-meal genetic profile to the villagers’ genetic profiles. Malaria infection in humans was determined by PCR test of blood samples. The results show nonrandom distribution of blood feeding among humans, with biased selection toward males and individuals aged 15–30 years. Prevalence of Plasmodium falciparum infection was higher in this age group, suggesting males in this age range could be super-spreaders of malaria parasites. The proportion of mosquitoes that fed on multiple humans ranged from 6% to 13% among villages. The patterns of host utilization observed here can amplify transmission and contribute to the persistence of malaria in PNG despite efforts to suppress it with insecticidal bed nets. Excessive feeding on males aged 15–30 years underscores the importance of targeted interventions focusing on this demographic group. The American Society of Tropical Medicine and Hygiene 2021-12 2021-09-27 /pmc/articles/PMC8641310/ /pubmed/34583342 http://dx.doi.org/10.4269/ajtmh.21-0210 Text en © The American Society of Tropical Medicine and Hygiene https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Articles
Keven, John B.
Katusele, Michelle
Vinit, Rebecca
Rodríguez-Rodríguez, Daniela
Hetzel, Manuel W.
Robinson, Leanne J.
Laman, Moses
Karl, Stephan
Foran, David R.
Walker, Edward D.
Nonrandom Selection and Multiple Blood Feeding of Human Hosts by Anopheles Vectors: Implications for Malaria Transmission in Papua New Guinea
title Nonrandom Selection and Multiple Blood Feeding of Human Hosts by Anopheles Vectors: Implications for Malaria Transmission in Papua New Guinea
title_full Nonrandom Selection and Multiple Blood Feeding of Human Hosts by Anopheles Vectors: Implications for Malaria Transmission in Papua New Guinea
title_fullStr Nonrandom Selection and Multiple Blood Feeding of Human Hosts by Anopheles Vectors: Implications for Malaria Transmission in Papua New Guinea
title_full_unstemmed Nonrandom Selection and Multiple Blood Feeding of Human Hosts by Anopheles Vectors: Implications for Malaria Transmission in Papua New Guinea
title_short Nonrandom Selection and Multiple Blood Feeding of Human Hosts by Anopheles Vectors: Implications for Malaria Transmission in Papua New Guinea
title_sort nonrandom selection and multiple blood feeding of human hosts by anopheles vectors: implications for malaria transmission in papua new guinea
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8641310/
https://www.ncbi.nlm.nih.gov/pubmed/34583342
http://dx.doi.org/10.4269/ajtmh.21-0210
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