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Evaluation of anticancer activity of Clerodendrum viscosum leaves against breast carcinoma
INTRODUCTION: The use of natural resources as medicines for cancer therapies has been described throughout history in the form of traditional medicines. However, many resources are still unidentified for their potent biological activities. Clerodendrum viscosum is a hill glory bower reported as a re...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8641747/ https://www.ncbi.nlm.nih.gov/pubmed/34854406 http://dx.doi.org/10.4103/ijp.IJP_565_19 |
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author | Shendge, Anil Khushalrao Basu, Tapasree Mandal, Nripendranath |
author_facet | Shendge, Anil Khushalrao Basu, Tapasree Mandal, Nripendranath |
author_sort | Shendge, Anil Khushalrao |
collection | PubMed |
description | INTRODUCTION: The use of natural resources as medicines for cancer therapies has been described throughout history in the form of traditional medicines. However, many resources are still unidentified for their potent biological activities. Clerodendrum viscosum is a hill glory bower reported as a remedy against oxidative stress, skin diseases, and intestinal infections. MATERIALS AND METHODS: We have collected the C. viscosum leaves and used for the preparation of 70% methanolic extract (CVLME). Then, CVLME has been confirmed for anticancer properties on various cancer cell lines by evaluating cytotoxicity, cell cycle analysis, induction of ROS and apoptosis, and nuclear fragmentation. Further, the phytochemical analysis of CVLME was evaluated through high-performance liquid chromatography. RESULTS: Cell proliferation assay revealed the selective cytotoxicity of CVLME against breast cancer cell line (MCF-7). The FACS-based cell cycle analysis showed increased subG1 (apoptosis) population dose dependently. Further, the apoptosis-inducing effect of CVLME was confirmed by annexin staining. Flow cytometry and confocal microscopy revealed the selective ROS generation upon CVLME treatment. The confocal-based morphological study also revealed condensed and fragmented nuclear structure in CVLME-treated MCF-7 cells. Phytochemical investigations further indicated the presence of tannic acid, catechin, rutin, and reserpine which might be the reason for the anticancer activity of CVLME. CONCLUSION: The above-combined results revealed the anticancer effect of CVLME, which may be due to the selective induction of ROS in breast carcinoma. |
format | Online Article Text |
id | pubmed-8641747 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-86417472021-12-14 Evaluation of anticancer activity of Clerodendrum viscosum leaves against breast carcinoma Shendge, Anil Khushalrao Basu, Tapasree Mandal, Nripendranath Indian J Pharmacol Experimental Research Article INTRODUCTION: The use of natural resources as medicines for cancer therapies has been described throughout history in the form of traditional medicines. However, many resources are still unidentified for their potent biological activities. Clerodendrum viscosum is a hill glory bower reported as a remedy against oxidative stress, skin diseases, and intestinal infections. MATERIALS AND METHODS: We have collected the C. viscosum leaves and used for the preparation of 70% methanolic extract (CVLME). Then, CVLME has been confirmed for anticancer properties on various cancer cell lines by evaluating cytotoxicity, cell cycle analysis, induction of ROS and apoptosis, and nuclear fragmentation. Further, the phytochemical analysis of CVLME was evaluated through high-performance liquid chromatography. RESULTS: Cell proliferation assay revealed the selective cytotoxicity of CVLME against breast cancer cell line (MCF-7). The FACS-based cell cycle analysis showed increased subG1 (apoptosis) population dose dependently. Further, the apoptosis-inducing effect of CVLME was confirmed by annexin staining. Flow cytometry and confocal microscopy revealed the selective ROS generation upon CVLME treatment. The confocal-based morphological study also revealed condensed and fragmented nuclear structure in CVLME-treated MCF-7 cells. Phytochemical investigations further indicated the presence of tannic acid, catechin, rutin, and reserpine which might be the reason for the anticancer activity of CVLME. CONCLUSION: The above-combined results revealed the anticancer effect of CVLME, which may be due to the selective induction of ROS in breast carcinoma. Wolters Kluwer - Medknow 2021 2021-11-24 /pmc/articles/PMC8641747/ /pubmed/34854406 http://dx.doi.org/10.4103/ijp.IJP_565_19 Text en Copyright: © 2021 Indian Journal of Pharmacology https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Experimental Research Article Shendge, Anil Khushalrao Basu, Tapasree Mandal, Nripendranath Evaluation of anticancer activity of Clerodendrum viscosum leaves against breast carcinoma |
title | Evaluation of anticancer activity of Clerodendrum viscosum leaves against breast carcinoma |
title_full | Evaluation of anticancer activity of Clerodendrum viscosum leaves against breast carcinoma |
title_fullStr | Evaluation of anticancer activity of Clerodendrum viscosum leaves against breast carcinoma |
title_full_unstemmed | Evaluation of anticancer activity of Clerodendrum viscosum leaves against breast carcinoma |
title_short | Evaluation of anticancer activity of Clerodendrum viscosum leaves against breast carcinoma |
title_sort | evaluation of anticancer activity of clerodendrum viscosum leaves against breast carcinoma |
topic | Experimental Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8641747/ https://www.ncbi.nlm.nih.gov/pubmed/34854406 http://dx.doi.org/10.4103/ijp.IJP_565_19 |
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