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CRISPR-Cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes
Animals are essential genetic tools in scientific research and global resources in agriculture. In both arenas, a single sex is often required in surplus. The ethical and financial burden of producing and culling animals of the undesired sex is considerable. Using the mouse as a model, we develop a...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8642469/ https://www.ncbi.nlm.nih.gov/pubmed/34862376 http://dx.doi.org/10.1038/s41467-021-27227-2 |
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author | Douglas, Charlotte Maciulyte, Valdone Zohren, Jasmin Snell, Daniel M. Mahadevaiah, Shantha K. Ojarikre, Obah A. Ellis, Peter J. I. Turner, James M. A. |
author_facet | Douglas, Charlotte Maciulyte, Valdone Zohren, Jasmin Snell, Daniel M. Mahadevaiah, Shantha K. Ojarikre, Obah A. Ellis, Peter J. I. Turner, James M. A. |
author_sort | Douglas, Charlotte |
collection | PubMed |
description | Animals are essential genetic tools in scientific research and global resources in agriculture. In both arenas, a single sex is often required in surplus. The ethical and financial burden of producing and culling animals of the undesired sex is considerable. Using the mouse as a model, we develop a synthetic lethal, bicomponent CRISPR-Cas9 strategy that produces male- or female-only litters with one hundred percent efficiency. Strikingly, we observe a degree of litter size compensation relative to control matings, indicating that our system has the potential to increase the yield of the desired sex in comparison to standard breeding designs. The bicomponent system can also be repurposed to generate postnatal sex-specific phenotypes. Our approach, harnessing the technological applications of CRISPR-Cas9, may be applicable to other vertebrate species, and provides strides towards ethical improvements for laboratory research and agriculture. |
format | Online Article Text |
id | pubmed-8642469 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-86424692021-12-15 CRISPR-Cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes Douglas, Charlotte Maciulyte, Valdone Zohren, Jasmin Snell, Daniel M. Mahadevaiah, Shantha K. Ojarikre, Obah A. Ellis, Peter J. I. Turner, James M. A. Nat Commun Article Animals are essential genetic tools in scientific research and global resources in agriculture. In both arenas, a single sex is often required in surplus. The ethical and financial burden of producing and culling animals of the undesired sex is considerable. Using the mouse as a model, we develop a synthetic lethal, bicomponent CRISPR-Cas9 strategy that produces male- or female-only litters with one hundred percent efficiency. Strikingly, we observe a degree of litter size compensation relative to control matings, indicating that our system has the potential to increase the yield of the desired sex in comparison to standard breeding designs. The bicomponent system can also be repurposed to generate postnatal sex-specific phenotypes. Our approach, harnessing the technological applications of CRISPR-Cas9, may be applicable to other vertebrate species, and provides strides towards ethical improvements for laboratory research and agriculture. Nature Publishing Group UK 2021-12-03 /pmc/articles/PMC8642469/ /pubmed/34862376 http://dx.doi.org/10.1038/s41467-021-27227-2 Text en © Crown 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Douglas, Charlotte Maciulyte, Valdone Zohren, Jasmin Snell, Daniel M. Mahadevaiah, Shantha K. Ojarikre, Obah A. Ellis, Peter J. I. Turner, James M. A. CRISPR-Cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes |
title | CRISPR-Cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes |
title_full | CRISPR-Cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes |
title_fullStr | CRISPR-Cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes |
title_full_unstemmed | CRISPR-Cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes |
title_short | CRISPR-Cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes |
title_sort | crispr-cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8642469/ https://www.ncbi.nlm.nih.gov/pubmed/34862376 http://dx.doi.org/10.1038/s41467-021-27227-2 |
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