A comprehensive comparison between camelid nanobodies and single chain variable fragments

By the emergence of recombinant DNA technology, many antibody fragments have been developed devoid of undesired properties of natural immunoglobulins. Among them, camelid heavy-chain variable domains (VHHs) and single-chain variable fragments (scFvs) are the most favored ones. While scFv is used wid...

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Autores principales: Asaadi, Yasaman, Jouneghani, Fatemeh Fazlollahi, Janani, Sara, Rahbarizadeh, Fatemeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8642758/
https://www.ncbi.nlm.nih.gov/pubmed/34863296
http://dx.doi.org/10.1186/s40364-021-00332-6
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author Asaadi, Yasaman
Jouneghani, Fatemeh Fazlollahi
Janani, Sara
Rahbarizadeh, Fatemeh
author_facet Asaadi, Yasaman
Jouneghani, Fatemeh Fazlollahi
Janani, Sara
Rahbarizadeh, Fatemeh
author_sort Asaadi, Yasaman
collection PubMed
description By the emergence of recombinant DNA technology, many antibody fragments have been developed devoid of undesired properties of natural immunoglobulins. Among them, camelid heavy-chain variable domains (VHHs) and single-chain variable fragments (scFvs) are the most favored ones. While scFv is used widely in various applications, camelid antibodies (VHHs) can serve as an alternative because of their superior chemical and physical properties such as higher solubility, stability, smaller size, and lower production cost. Here, these two counterparts are compared in structure and properties to identify which one is more suitable for each of their various therapeutic, diagnosis, and research applications.
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spelling pubmed-86427582021-12-06 A comprehensive comparison between camelid nanobodies and single chain variable fragments Asaadi, Yasaman Jouneghani, Fatemeh Fazlollahi Janani, Sara Rahbarizadeh, Fatemeh Biomark Res Review By the emergence of recombinant DNA technology, many antibody fragments have been developed devoid of undesired properties of natural immunoglobulins. Among them, camelid heavy-chain variable domains (VHHs) and single-chain variable fragments (scFvs) are the most favored ones. While scFv is used widely in various applications, camelid antibodies (VHHs) can serve as an alternative because of their superior chemical and physical properties such as higher solubility, stability, smaller size, and lower production cost. Here, these two counterparts are compared in structure and properties to identify which one is more suitable for each of their various therapeutic, diagnosis, and research applications. BioMed Central 2021-12-04 /pmc/articles/PMC8642758/ /pubmed/34863296 http://dx.doi.org/10.1186/s40364-021-00332-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Review
Asaadi, Yasaman
Jouneghani, Fatemeh Fazlollahi
Janani, Sara
Rahbarizadeh, Fatemeh
A comprehensive comparison between camelid nanobodies and single chain variable fragments
title A comprehensive comparison between camelid nanobodies and single chain variable fragments
title_full A comprehensive comparison between camelid nanobodies and single chain variable fragments
title_fullStr A comprehensive comparison between camelid nanobodies and single chain variable fragments
title_full_unstemmed A comprehensive comparison between camelid nanobodies and single chain variable fragments
title_short A comprehensive comparison between camelid nanobodies and single chain variable fragments
title_sort comprehensive comparison between camelid nanobodies and single chain variable fragments
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8642758/
https://www.ncbi.nlm.nih.gov/pubmed/34863296
http://dx.doi.org/10.1186/s40364-021-00332-6
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