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Efficient DNA interrogation of SpCas9 governed by its electrostatic interaction with DNA beyond the PAM and protospacer

Streptococcus pyogenes Cas9 (SpCas9), a programmable RNA-guided DNA endonuclease, has been widely repurposed for biological and medical applications. Critical interactions between SpCas9 and DNA confer the high specificity of the enzyme in genome engineering. Here, we unveil that an essential SpCas9...

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Detalles Bibliográficos
Autores principales:	Zhang, Qian, Chen, Ziting, Wang, Fangzhu, Zhang, Siqi, Chen, Hongyu, Gu, Xueying, Wen, Fengcai, Jin, Jiachuan, Zhang, Xia, Huang, Xingxu, Shen, Bin, Sun, Bo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2021
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8643646/ https://www.ncbi.nlm.nih.gov/pubmed/34850124 http://dx.doi.org/10.1093/nar/gkab1139

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