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1333. A National Outbreak of Respiratory Syncytial Virus Associated with Emergence of a Genetically Distinct ON1 variant
BACKGROUND: A national outbreak of respiratory syncytial virus (RSV) has been observed in the community since Fall 2020 in Taiwan. METHODS: We reviewed a national laboratory-based surveillance network established over 20 years by Taiwan Centers for Disease Control (TCDC) for respiratory viral positi...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8643801/ http://dx.doi.org/10.1093/ofid/ofab466.1525 |
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author | Lin, Wei Hsuan Wu, Fang-Tzy Chen, Yi-Yin Tsao, Kuo Chien Hsieh, Yu-Chia |
author_facet | Lin, Wei Hsuan Wu, Fang-Tzy Chen, Yi-Yin Tsao, Kuo Chien Hsieh, Yu-Chia |
author_sort | Lin, Wei Hsuan |
collection | PubMed |
description | BACKGROUND: A national outbreak of respiratory syncytial virus (RSV) has been observed in the community since Fall 2020 in Taiwan. METHODS: We reviewed a national laboratory-based surveillance network established over 20 years by Taiwan Centers for Disease Control (TCDC) for respiratory viral positivity rate and viral pathogen in outpatient department and hospitalized patients. A retrospective study of children younger than 5 years old hospitalized with RSV infections at Chang Gung Memorial Hospital (CGMH) including Lin Kou and Kaohsiung branch between 2018 and 2020 was conducted. Samples positive for RSV A were sequenced. Patients’ clinical data was obtained from medical files and stratified by genotype and year. RESULTS: 2020 showed a 4-fold surge in RSV cases in Taiwan, in which surpassed both 2011 (the appearance of ON1 strain in Taiwan) and 2013 (ON1 strain predominates in Taiwan)(Figure1,Table1). Phylogenetic analysis of G protein sequences showed that most strains in 2020 were clustered apart from 2018, 2019 seasons and other ON1 reference strains between 2011 and 2016, indicating a novel ON1 variant had been circulating in the community(Figure2). The novel ON1 variants carried six amino acid changes, of which T113I,V131D, H258Q,H266L,Y304H located in the mucin domains and N178G in central conserved domain. These changes emerged gradually in 2019 and showed a high consistency in 2020. The unique amino acid substitution E257K in the second mucin domain was noticed in 2020 exclusively. Besides, 10 substitutes in F protein were appeared between 2018 and 2020, of which R213S and N276S were in antigenic sites. Furthermore, substitutes T12I and H514N in F protein were first emerged since 2020. On multivariate analysis, age (OR 0.97; 95% CI 0.94-0.99; p 0.02) and ON1 variant in 2020 (OR 2.52; 95% CI 1.13-5.63; p 0.025) were independently associated with oxygen saturation < 94% during hospitalization (Table 2). [Image: see text] Figure 2. The phylogenetic tree of G protein sequences including ON1 strains in our study from 2018 to 2020 and reference strains from 2011 to 2016 [Image: see text] The phylogenetic tree showed that most strains of the 2020 season clustered apart from those of the previous seasons, which indicated there was a novel ON1 variant circulating in the community associated with 2020 RSV epidemic. [Image: see text] Variables with p<0.1 in the univariate analysis were included in the multivariate analysis. CONCLUSION: An unprecedented RSV epidemic within the last 10 years caused by a novel ON1 variant has occurred in 2020 (Figure 1), suggesting the sets of mutations may confer fitness advantage. Further studies on viral replication, infectivity and virulence is needed to understand the evolution and spread of RSV. [Image: see text] DISCLOSURES: All Authors: No reported disclosures |
format | Online Article Text |
id | pubmed-8643801 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-86438012021-12-06 1333. A National Outbreak of Respiratory Syncytial Virus Associated with Emergence of a Genetically Distinct ON1 variant Lin, Wei Hsuan Wu, Fang-Tzy Chen, Yi-Yin Tsao, Kuo Chien Hsieh, Yu-Chia Open Forum Infect Dis Poster Abstracts BACKGROUND: A national outbreak of respiratory syncytial virus (RSV) has been observed in the community since Fall 2020 in Taiwan. METHODS: We reviewed a national laboratory-based surveillance network established over 20 years by Taiwan Centers for Disease Control (TCDC) for respiratory viral positivity rate and viral pathogen in outpatient department and hospitalized patients. A retrospective study of children younger than 5 years old hospitalized with RSV infections at Chang Gung Memorial Hospital (CGMH) including Lin Kou and Kaohsiung branch between 2018 and 2020 was conducted. Samples positive for RSV A were sequenced. Patients’ clinical data was obtained from medical files and stratified by genotype and year. RESULTS: 2020 showed a 4-fold surge in RSV cases in Taiwan, in which surpassed both 2011 (the appearance of ON1 strain in Taiwan) and 2013 (ON1 strain predominates in Taiwan)(Figure1,Table1). Phylogenetic analysis of G protein sequences showed that most strains in 2020 were clustered apart from 2018, 2019 seasons and other ON1 reference strains between 2011 and 2016, indicating a novel ON1 variant had been circulating in the community(Figure2). The novel ON1 variants carried six amino acid changes, of which T113I,V131D, H258Q,H266L,Y304H located in the mucin domains and N178G in central conserved domain. These changes emerged gradually in 2019 and showed a high consistency in 2020. The unique amino acid substitution E257K in the second mucin domain was noticed in 2020 exclusively. Besides, 10 substitutes in F protein were appeared between 2018 and 2020, of which R213S and N276S were in antigenic sites. Furthermore, substitutes T12I and H514N in F protein were first emerged since 2020. On multivariate analysis, age (OR 0.97; 95% CI 0.94-0.99; p 0.02) and ON1 variant in 2020 (OR 2.52; 95% CI 1.13-5.63; p 0.025) were independently associated with oxygen saturation < 94% during hospitalization (Table 2). [Image: see text] Figure 2. The phylogenetic tree of G protein sequences including ON1 strains in our study from 2018 to 2020 and reference strains from 2011 to 2016 [Image: see text] The phylogenetic tree showed that most strains of the 2020 season clustered apart from those of the previous seasons, which indicated there was a novel ON1 variant circulating in the community associated with 2020 RSV epidemic. [Image: see text] Variables with p<0.1 in the univariate analysis were included in the multivariate analysis. CONCLUSION: An unprecedented RSV epidemic within the last 10 years caused by a novel ON1 variant has occurred in 2020 (Figure 1), suggesting the sets of mutations may confer fitness advantage. Further studies on viral replication, infectivity and virulence is needed to understand the evolution and spread of RSV. [Image: see text] DISCLOSURES: All Authors: No reported disclosures Oxford University Press 2021-12-04 /pmc/articles/PMC8643801/ http://dx.doi.org/10.1093/ofid/ofab466.1525 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Infectious Diseases Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Poster Abstracts Lin, Wei Hsuan Wu, Fang-Tzy Chen, Yi-Yin Tsao, Kuo Chien Hsieh, Yu-Chia 1333. A National Outbreak of Respiratory Syncytial Virus Associated with Emergence of a Genetically Distinct ON1 variant |
title | 1333. A National Outbreak of Respiratory Syncytial Virus Associated with Emergence of a Genetically Distinct ON1 variant |
title_full | 1333. A National Outbreak of Respiratory Syncytial Virus Associated with Emergence of a Genetically Distinct ON1 variant |
title_fullStr | 1333. A National Outbreak of Respiratory Syncytial Virus Associated with Emergence of a Genetically Distinct ON1 variant |
title_full_unstemmed | 1333. A National Outbreak of Respiratory Syncytial Virus Associated with Emergence of a Genetically Distinct ON1 variant |
title_short | 1333. A National Outbreak of Respiratory Syncytial Virus Associated with Emergence of a Genetically Distinct ON1 variant |
title_sort | 1333. a national outbreak of respiratory syncytial virus associated with emergence of a genetically distinct on1 variant |
topic | Poster Abstracts |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8643801/ http://dx.doi.org/10.1093/ofid/ofab466.1525 |
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