Cargando…

453. Neutralizing Antibody Responses to SARS-CoV-2 in Professional Soccer Players

BACKGROUND: The Brazilian Football Confederation (CBF) protocol to control the spread of COVID-19 among professional soccer players is based on four cornerstone measures: (1) Tracing all symptomatic and asymptomatic COVID-19 cases by clinical monitoring and nasal swab SARS-CoV-2 RT-PCR testing up to...

Descripción completa

Detalles Bibliográficos
Autores principales: Pagura, Jorge, de Cunha, Clovis Arns, Nishimura, Roberto, Wey, Sergio, Pedrinelli, André, Cimerman, Sergio, Picanço, Andreia, Freire, Sérgio, Guerreiro, André, Thiele, Edilson, Starling, Carlos, Couto, Bráulio R G M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8643837/
http://dx.doi.org/10.1093/ofid/ofab466.652
_version_ 1784609945636831232
author Pagura, Jorge
de Cunha, Clovis Arns
Nishimura, Roberto
Wey, Sergio
Pedrinelli, André
Cimerman, Sergio
Picanço, Andreia
Freire, Sérgio
Guerreiro, André
Thiele, Edilson
Starling, Carlos
Couto, Bráulio R G M
author_facet Pagura, Jorge
de Cunha, Clovis Arns
Nishimura, Roberto
Wey, Sergio
Pedrinelli, André
Cimerman, Sergio
Picanço, Andreia
Freire, Sérgio
Guerreiro, André
Thiele, Edilson
Starling, Carlos
Couto, Bráulio R G M
author_sort Pagura, Jorge
collection PubMed
description BACKGROUND: The Brazilian Football Confederation (CBF) protocol to control the spread of COVID-19 among professional soccer players is based on four cornerstone measures: (1) Tracing all symptomatic and asymptomatic COVID-19 cases by clinical monitoring and nasal swab SARS-CoV-2 RT-PCR testing up to 3 days before the soccer games; (2) Respiratory isolation of all SARS-CoV-2 positive players for at least 10 days, regardless symptoms; (3) All player with clinical suspicion of COVID-19 were immediately quarantined; (4) If a player became SARS-CoV-2 positive after the game, the other players were allowed to play the next game, if they remained asymptomatic and SARS-CoV-2 RT-PCR negative. Understanding how antibody responses to SARS-CoV-2 evolve can provide insights into therapeutic and testing approaches for COVID-19. In the present study we profile the antibody responses of players up to nine months from a SARS-CoV-2 positive RT-PCR test. METHODS: Serum samples were obtained from 955 soccer players, and analyzed at the same laboratory in São Paulo city, in the Hospital Israelita Albert Einstein. It was used the cPas Technology, the sVNT kit for detecting and measuring circulating neutralizing antibodies against the SARS-CoV-2 virus. RESULTS: Neutralizing antibody was positive for 416 samples (416/955=44%; C.I. 95%= [40%; 47%]). From the 955 soccer players, 454 had RT-PCR+ previously, up to nine months until the neutralizing antibody tests. From this 454 players, 172 (38%) had neutralizing antibody below 20% (C.I. 95% = [34%; 42%]), 30 (7%) between 20% and 30% (C.I. 95% = [5%; 9%]), and e 252 (56%) above 30% (C.I. 95% =[51%; 60%]). Antibody responses to SARS-CoV-2 were significantly higher in individuals RT-PCR+ (Table 1). There was no difference between the neutralizing antibody responses status to SARS-CoV-2 and the time between the RT-PCR+ and the neutralizing antibody test (p-value = 0.423; Figures 1 and 2, Table 2). Table 1. Neutralizing antibody responses to SARS-CoV-2. [Image: see text] Figure 1. Scatter plot with Time between RT-PCR+ and neutralizing antibody (days) versus Neutralizing antibody levels. [Image: see text] Table 2. Time between RT-PCR+ and neutralizing antibody (days) versus Neutralizing antibody levels. [Image: see text] CONCLUSION: This study found neutralizing activity of infection against SARS-CoV-2 in 63% RT-PCR+ individuals, but only in 26% in RT-PCR(-) players. Level of neutralizing antibody responses maintained stable until up to nine months after a RT-PCR+. Figure 2. Percentage of soccer players at each antibody level (below 20%, between 20% and 30%, and above 30%) versus time between the positive RT-PCR test and neutralizing antibody test (days). [Image: see text] DISCLOSURES: All Authors: No reported disclosures
format Online
Article
Text
id pubmed-8643837
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-86438372021-12-06 453. Neutralizing Antibody Responses to SARS-CoV-2 in Professional Soccer Players Pagura, Jorge de Cunha, Clovis Arns Nishimura, Roberto Wey, Sergio Pedrinelli, André Cimerman, Sergio Picanço, Andreia Freire, Sérgio Guerreiro, André Thiele, Edilson Starling, Carlos Couto, Bráulio R G M Open Forum Infect Dis Poster Abstracts BACKGROUND: The Brazilian Football Confederation (CBF) protocol to control the spread of COVID-19 among professional soccer players is based on four cornerstone measures: (1) Tracing all symptomatic and asymptomatic COVID-19 cases by clinical monitoring and nasal swab SARS-CoV-2 RT-PCR testing up to 3 days before the soccer games; (2) Respiratory isolation of all SARS-CoV-2 positive players for at least 10 days, regardless symptoms; (3) All player with clinical suspicion of COVID-19 were immediately quarantined; (4) If a player became SARS-CoV-2 positive after the game, the other players were allowed to play the next game, if they remained asymptomatic and SARS-CoV-2 RT-PCR negative. Understanding how antibody responses to SARS-CoV-2 evolve can provide insights into therapeutic and testing approaches for COVID-19. In the present study we profile the antibody responses of players up to nine months from a SARS-CoV-2 positive RT-PCR test. METHODS: Serum samples were obtained from 955 soccer players, and analyzed at the same laboratory in São Paulo city, in the Hospital Israelita Albert Einstein. It was used the cPas Technology, the sVNT kit for detecting and measuring circulating neutralizing antibodies against the SARS-CoV-2 virus. RESULTS: Neutralizing antibody was positive for 416 samples (416/955=44%; C.I. 95%= [40%; 47%]). From the 955 soccer players, 454 had RT-PCR+ previously, up to nine months until the neutralizing antibody tests. From this 454 players, 172 (38%) had neutralizing antibody below 20% (C.I. 95% = [34%; 42%]), 30 (7%) between 20% and 30% (C.I. 95% = [5%; 9%]), and e 252 (56%) above 30% (C.I. 95% =[51%; 60%]). Antibody responses to SARS-CoV-2 were significantly higher in individuals RT-PCR+ (Table 1). There was no difference between the neutralizing antibody responses status to SARS-CoV-2 and the time between the RT-PCR+ and the neutralizing antibody test (p-value = 0.423; Figures 1 and 2, Table 2). Table 1. Neutralizing antibody responses to SARS-CoV-2. [Image: see text] Figure 1. Scatter plot with Time between RT-PCR+ and neutralizing antibody (days) versus Neutralizing antibody levels. [Image: see text] Table 2. Time between RT-PCR+ and neutralizing antibody (days) versus Neutralizing antibody levels. [Image: see text] CONCLUSION: This study found neutralizing activity of infection against SARS-CoV-2 in 63% RT-PCR+ individuals, but only in 26% in RT-PCR(-) players. Level of neutralizing antibody responses maintained stable until up to nine months after a RT-PCR+. Figure 2. Percentage of soccer players at each antibody level (below 20%, between 20% and 30%, and above 30%) versus time between the positive RT-PCR test and neutralizing antibody test (days). [Image: see text] DISCLOSURES: All Authors: No reported disclosures Oxford University Press 2021-12-04 /pmc/articles/PMC8643837/ http://dx.doi.org/10.1093/ofid/ofab466.652 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Infectious Diseases Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Poster Abstracts
Pagura, Jorge
de Cunha, Clovis Arns
Nishimura, Roberto
Wey, Sergio
Pedrinelli, André
Cimerman, Sergio
Picanço, Andreia
Freire, Sérgio
Guerreiro, André
Thiele, Edilson
Starling, Carlos
Couto, Bráulio R G M
453. Neutralizing Antibody Responses to SARS-CoV-2 in Professional Soccer Players
title 453. Neutralizing Antibody Responses to SARS-CoV-2 in Professional Soccer Players
title_full 453. Neutralizing Antibody Responses to SARS-CoV-2 in Professional Soccer Players
title_fullStr 453. Neutralizing Antibody Responses to SARS-CoV-2 in Professional Soccer Players
title_full_unstemmed 453. Neutralizing Antibody Responses to SARS-CoV-2 in Professional Soccer Players
title_short 453. Neutralizing Antibody Responses to SARS-CoV-2 in Professional Soccer Players
title_sort 453. neutralizing antibody responses to sars-cov-2 in professional soccer players
topic Poster Abstracts
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8643837/
http://dx.doi.org/10.1093/ofid/ofab466.652
work_keys_str_mv AT pagurajorge 453neutralizingantibodyresponsestosarscov2inprofessionalsoccerplayers
AT decunhaclovisarns 453neutralizingantibodyresponsestosarscov2inprofessionalsoccerplayers
AT nishimuraroberto 453neutralizingantibodyresponsestosarscov2inprofessionalsoccerplayers
AT weysergio 453neutralizingantibodyresponsestosarscov2inprofessionalsoccerplayers
AT pedrinelliandre 453neutralizingantibodyresponsestosarscov2inprofessionalsoccerplayers
AT cimermansergio 453neutralizingantibodyresponsestosarscov2inprofessionalsoccerplayers
AT picancoandreia 453neutralizingantibodyresponsestosarscov2inprofessionalsoccerplayers
AT freiresergio 453neutralizingantibodyresponsestosarscov2inprofessionalsoccerplayers
AT guerreiroandre 453neutralizingantibodyresponsestosarscov2inprofessionalsoccerplayers
AT thieleedilson 453neutralizingantibodyresponsestosarscov2inprofessionalsoccerplayers
AT starlingcarlos 453neutralizingantibodyresponsestosarscov2inprofessionalsoccerplayers
AT coutobrauliorgm 453neutralizingantibodyresponsestosarscov2inprofessionalsoccerplayers