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799. A Pseudo-Outbreak of Pseudomonas fluorescens Infections
BACKGROUND: Pseudomonas fluorescens is a water-borne pathogen that has been associated with outbreaks from transfusion of contaminated blood products or medical equipment. Our institution had a cluster of cultures that grew an uncommonly encountered microbe P. fluorescens within a period of one week...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8644160/ http://dx.doi.org/10.1093/ofid/ofab466.995 |
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author | Jaber, Tariq Saini, Vikram Morris, Laura Como, James D Bhanot, Nitin Min, Zaw |
author_facet | Jaber, Tariq Saini, Vikram Morris, Laura Como, James D Bhanot, Nitin Min, Zaw |
author_sort | Jaber, Tariq |
collection | PubMed |
description | BACKGROUND: Pseudomonas fluorescens is a water-borne pathogen that has been associated with outbreaks from transfusion of contaminated blood products or medical equipment. Our institution had a cluster of cultures that grew an uncommonly encountered microbe P. fluorescens within a period of one week. This prompted an internal investigation. We summarize the investigational process that led to the resolution of this pseudo-outbreak. METHODS: We conducted a retrospective chart review of surgical and non-surgical patients with cultures positive for P. fluorescens from July 2(nd) to July 8(th) 2020. Baseline patient characteristics, clinical course, laboratory data, use of blood-associated products, and microbiology cultures were analyzed. RESULTS: Eight patients were identified with positive tissue cultures for P. fluorescens. Among those, 5 specimens (62.5%) were from osteoarticular sites (1 prosthetic hip, 1 prosthetic knee, 1 right foot, 1 sternum, and 1 vertebral source). One culture (12.5%) was obtained from a sacral soft tissue wound. Two tissue specimens (25%) were collected from respiratory sites (1 lung tissue and 1 bronchoalveolar lavage). No association with specific surgical personnel or operating room was identified. During routine specimen processing, a small amount of sterile normal saline is added to the conical grinder prior to culture preparation. It was discovered that a non-sterile normal saline had been inadvertently utilized during that step. These eight tissue specimens were subsequently reprocessed with sterile solution; P. fluorescens was not re-isolated. Specimen processing protocols were reinforced. Adjustment of antimicrobial therapy was made accordingly without reported subsequent adverse clinical outcomes. CONCLUSION: A multi-faceted team approach in collaboration with Infection Prevention, Infectious Diseases, Surgery, operating room personnel, and Microbiology identified an unintended breakdown in sterile laboratory protocols which resulted in a cluster of falsely positive cultures. An increased incidence of infection with an uncommon pathogen initiated a prompt investigation that resulted in the identification of a pseudo-outbreak event. DISCLOSURES: All Authors: No reported disclosures |
format | Online Article Text |
id | pubmed-8644160 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-86441602021-12-06 799. A Pseudo-Outbreak of Pseudomonas fluorescens Infections Jaber, Tariq Saini, Vikram Morris, Laura Como, James D Bhanot, Nitin Min, Zaw Open Forum Infect Dis Poster Abstracts BACKGROUND: Pseudomonas fluorescens is a water-borne pathogen that has been associated with outbreaks from transfusion of contaminated blood products or medical equipment. Our institution had a cluster of cultures that grew an uncommonly encountered microbe P. fluorescens within a period of one week. This prompted an internal investigation. We summarize the investigational process that led to the resolution of this pseudo-outbreak. METHODS: We conducted a retrospective chart review of surgical and non-surgical patients with cultures positive for P. fluorescens from July 2(nd) to July 8(th) 2020. Baseline patient characteristics, clinical course, laboratory data, use of blood-associated products, and microbiology cultures were analyzed. RESULTS: Eight patients were identified with positive tissue cultures for P. fluorescens. Among those, 5 specimens (62.5%) were from osteoarticular sites (1 prosthetic hip, 1 prosthetic knee, 1 right foot, 1 sternum, and 1 vertebral source). One culture (12.5%) was obtained from a sacral soft tissue wound. Two tissue specimens (25%) were collected from respiratory sites (1 lung tissue and 1 bronchoalveolar lavage). No association with specific surgical personnel or operating room was identified. During routine specimen processing, a small amount of sterile normal saline is added to the conical grinder prior to culture preparation. It was discovered that a non-sterile normal saline had been inadvertently utilized during that step. These eight tissue specimens were subsequently reprocessed with sterile solution; P. fluorescens was not re-isolated. Specimen processing protocols were reinforced. Adjustment of antimicrobial therapy was made accordingly without reported subsequent adverse clinical outcomes. CONCLUSION: A multi-faceted team approach in collaboration with Infection Prevention, Infectious Diseases, Surgery, operating room personnel, and Microbiology identified an unintended breakdown in sterile laboratory protocols which resulted in a cluster of falsely positive cultures. An increased incidence of infection with an uncommon pathogen initiated a prompt investigation that resulted in the identification of a pseudo-outbreak event. DISCLOSURES: All Authors: No reported disclosures Oxford University Press 2021-12-04 /pmc/articles/PMC8644160/ http://dx.doi.org/10.1093/ofid/ofab466.995 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Infectious Diseases Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Poster Abstracts Jaber, Tariq Saini, Vikram Morris, Laura Como, James D Bhanot, Nitin Min, Zaw 799. A Pseudo-Outbreak of Pseudomonas fluorescens Infections |
title | 799. A Pseudo-Outbreak of Pseudomonas fluorescens Infections |
title_full | 799. A Pseudo-Outbreak of Pseudomonas fluorescens Infections |
title_fullStr | 799. A Pseudo-Outbreak of Pseudomonas fluorescens Infections |
title_full_unstemmed | 799. A Pseudo-Outbreak of Pseudomonas fluorescens Infections |
title_short | 799. A Pseudo-Outbreak of Pseudomonas fluorescens Infections |
title_sort | 799. a pseudo-outbreak of pseudomonas fluorescens infections |
topic | Poster Abstracts |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8644160/ http://dx.doi.org/10.1093/ofid/ofab466.995 |
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