Co-targeting strategy for precise, scarless gene editing with CRISPR/Cas9 and donor ssODNs in Chlamydomonas

Programmable site-specific nucleases, such as the clustered regularly interspaced short palindromic repeat (CRISPR)/ CRISPR-associated protein 9 (Cas9) ribonucleoproteins (RNPs), have allowed creation of valuable knockout mutations and targeted gene modifications in Chlamydomonas (Chlamydomonas rein...

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Detalles Bibliográficos
Autores principales: Akella, Soujanya, Ma, Xinrong, Bacova, Romana, Harmer, Zachary P, Kolackova, Martina, Wen, Xiaoxue, Wright, David A, Spalding, Martin H, Weeks, Donald P, Cerutti, Heriberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Regular Issue
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8644747/
https://www.ncbi.nlm.nih.gov/pubmed/34618092
http://dx.doi.org/10.1093/plphys/kiab418

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