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PQN-59 and GTBP-1 contribute to stress granule formation but are not essential for their assembly in C. elegans embryos

When exposed to stressful conditions, eukaryotic cells respond by inducing the formation of cytoplasmic ribonucleoprotein complexes called stress granules. Here, we use C. elegans to study two proteins that are important for stress granule assembly in human cells – PQN-59, the human UBAP2L ortholog,...

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Autores principales: Abbatemarco, Simona, Bondaz, Alexandra, Schwager, Francoise, Wang, Jing, Hammell, Christopher M., Gotta, Monica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8645233/
https://www.ncbi.nlm.nih.gov/pubmed/34661238
http://dx.doi.org/10.1242/jcs.258834
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author Abbatemarco, Simona
Bondaz, Alexandra
Schwager, Francoise
Wang, Jing
Hammell, Christopher M.
Gotta, Monica
author_facet Abbatemarco, Simona
Bondaz, Alexandra
Schwager, Francoise
Wang, Jing
Hammell, Christopher M.
Gotta, Monica
author_sort Abbatemarco, Simona
collection PubMed
description When exposed to stressful conditions, eukaryotic cells respond by inducing the formation of cytoplasmic ribonucleoprotein complexes called stress granules. Here, we use C. elegans to study two proteins that are important for stress granule assembly in human cells – PQN-59, the human UBAP2L ortholog, and GTBP-1, the human G3BP1 and G3BP2 ortholog. Both proteins assemble into stress granules in the embryo and in the germline when C. elegans is exposed to stressful conditions. Neither of the two proteins is essential for the assembly of stress-induced granules, as shown by the single and combined depletions by RNAi, and neither pqn-59 nor gtbp-1 mutant embryos show higher sensitivity to stress than control embryos. We find that pqn-59 mutants display reduced progeny and a high percentage of embryonic lethality, phenotypes that are not dependent on stress exposure and that are not shared with gtbp-1 mutants. Our data indicate that, in contrast to human cells, PQN-59 and GTBP-1 are not required for stress granule formation but that PQN-59 is important for C. elegans development.
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spelling pubmed-86452332021-12-10 PQN-59 and GTBP-1 contribute to stress granule formation but are not essential for their assembly in C. elegans embryos Abbatemarco, Simona Bondaz, Alexandra Schwager, Francoise Wang, Jing Hammell, Christopher M. Gotta, Monica J Cell Sci Research Article When exposed to stressful conditions, eukaryotic cells respond by inducing the formation of cytoplasmic ribonucleoprotein complexes called stress granules. Here, we use C. elegans to study two proteins that are important for stress granule assembly in human cells – PQN-59, the human UBAP2L ortholog, and GTBP-1, the human G3BP1 and G3BP2 ortholog. Both proteins assemble into stress granules in the embryo and in the germline when C. elegans is exposed to stressful conditions. Neither of the two proteins is essential for the assembly of stress-induced granules, as shown by the single and combined depletions by RNAi, and neither pqn-59 nor gtbp-1 mutant embryos show higher sensitivity to stress than control embryos. We find that pqn-59 mutants display reduced progeny and a high percentage of embryonic lethality, phenotypes that are not dependent on stress exposure and that are not shared with gtbp-1 mutants. Our data indicate that, in contrast to human cells, PQN-59 and GTBP-1 are not required for stress granule formation but that PQN-59 is important for C. elegans development. The Company of Biologists Ltd 2021-11-15 /pmc/articles/PMC8645233/ /pubmed/34661238 http://dx.doi.org/10.1242/jcs.258834 Text en © 2021. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Abbatemarco, Simona
Bondaz, Alexandra
Schwager, Francoise
Wang, Jing
Hammell, Christopher M.
Gotta, Monica
PQN-59 and GTBP-1 contribute to stress granule formation but are not essential for their assembly in C. elegans embryos
title PQN-59 and GTBP-1 contribute to stress granule formation but are not essential for their assembly in C. elegans embryos
title_full PQN-59 and GTBP-1 contribute to stress granule formation but are not essential for their assembly in C. elegans embryos
title_fullStr PQN-59 and GTBP-1 contribute to stress granule formation but are not essential for their assembly in C. elegans embryos
title_full_unstemmed PQN-59 and GTBP-1 contribute to stress granule formation but are not essential for their assembly in C. elegans embryos
title_short PQN-59 and GTBP-1 contribute to stress granule formation but are not essential for their assembly in C. elegans embryos
title_sort pqn-59 and gtbp-1 contribute to stress granule formation but are not essential for their assembly in c. elegans embryos
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8645233/
https://www.ncbi.nlm.nih.gov/pubmed/34661238
http://dx.doi.org/10.1242/jcs.258834
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