ALKBH5 Is Lowly Expressed in Esophageal Squamous Cell Carcinoma and Inhibits the Malignant Proliferation and Invasion of Tumor Cells

BACKGROUND: Modification of N(6)-methyladenosine (m(6)A) and RNA m(6)A regulatory factors is required in cancer advancement. The contribution of m(6)A and its alteration in esophageal squamous cell carcinoma (ESCC) is still unclear. RESULTS: ALKBH5 was lowly expressed in ESCC tissues, which the tota...

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Autores principales: Li, Jinqiu, Liu, Hongqiang, Dong, Shanglin, Zhang, Yunbo, Li, Xiao, Wang, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8645391/
https://www.ncbi.nlm.nih.gov/pubmed/34876920
http://dx.doi.org/10.1155/2021/1001446
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author Li, Jinqiu
Liu, Hongqiang
Dong, Shanglin
Zhang, Yunbo
Li, Xiao
Wang, Jing
author_facet Li, Jinqiu
Liu, Hongqiang
Dong, Shanglin
Zhang, Yunbo
Li, Xiao
Wang, Jing
author_sort Li, Jinqiu
collection PubMed
description BACKGROUND: Modification of N(6)-methyladenosine (m(6)A) and RNA m(6)A regulatory factors is required in cancer advancement. The contribution of m(6)A and its alteration in esophageal squamous cell carcinoma (ESCC) is still unclear. RESULTS: ALKBH5 was lowly expressed in ESCC tissues, which the total m(6)A level was increased in ESCC tissue than the presentation in normal healthy tissue. The pcDNA3.1-ALKBH5 recombinant plasmid was transfected into KYSE-150 and Eca-109 cells. The overexpression of ALKBH5 is responsible for a significant reduction of the total m(6)A levels in Eca-109 and KYSE150 cells, inhibiting the proliferation capability, migration, and cell invasion. CONCLUSIONS: ALKBH5 as a demethylase was lowly expressed in cancer progression of ESCC and acts as a crucial component in ESCC progression.
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spelling pubmed-86453912021-12-06 ALKBH5 Is Lowly Expressed in Esophageal Squamous Cell Carcinoma and Inhibits the Malignant Proliferation and Invasion of Tumor Cells Li, Jinqiu Liu, Hongqiang Dong, Shanglin Zhang, Yunbo Li, Xiao Wang, Jing Comput Math Methods Med Research Article BACKGROUND: Modification of N(6)-methyladenosine (m(6)A) and RNA m(6)A regulatory factors is required in cancer advancement. The contribution of m(6)A and its alteration in esophageal squamous cell carcinoma (ESCC) is still unclear. RESULTS: ALKBH5 was lowly expressed in ESCC tissues, which the total m(6)A level was increased in ESCC tissue than the presentation in normal healthy tissue. The pcDNA3.1-ALKBH5 recombinant plasmid was transfected into KYSE-150 and Eca-109 cells. The overexpression of ALKBH5 is responsible for a significant reduction of the total m(6)A levels in Eca-109 and KYSE150 cells, inhibiting the proliferation capability, migration, and cell invasion. CONCLUSIONS: ALKBH5 as a demethylase was lowly expressed in cancer progression of ESCC and acts as a crucial component in ESCC progression. Hindawi 2021-11-28 /pmc/articles/PMC8645391/ /pubmed/34876920 http://dx.doi.org/10.1155/2021/1001446 Text en Copyright © 2021 Jinqiu Li et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Jinqiu
Liu, Hongqiang
Dong, Shanglin
Zhang, Yunbo
Li, Xiao
Wang, Jing
ALKBH5 Is Lowly Expressed in Esophageal Squamous Cell Carcinoma and Inhibits the Malignant Proliferation and Invasion of Tumor Cells
title ALKBH5 Is Lowly Expressed in Esophageal Squamous Cell Carcinoma and Inhibits the Malignant Proliferation and Invasion of Tumor Cells
title_full ALKBH5 Is Lowly Expressed in Esophageal Squamous Cell Carcinoma and Inhibits the Malignant Proliferation and Invasion of Tumor Cells
title_fullStr ALKBH5 Is Lowly Expressed in Esophageal Squamous Cell Carcinoma and Inhibits the Malignant Proliferation and Invasion of Tumor Cells
title_full_unstemmed ALKBH5 Is Lowly Expressed in Esophageal Squamous Cell Carcinoma and Inhibits the Malignant Proliferation and Invasion of Tumor Cells
title_short ALKBH5 Is Lowly Expressed in Esophageal Squamous Cell Carcinoma and Inhibits the Malignant Proliferation and Invasion of Tumor Cells
title_sort alkbh5 is lowly expressed in esophageal squamous cell carcinoma and inhibits the malignant proliferation and invasion of tumor cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8645391/
https://www.ncbi.nlm.nih.gov/pubmed/34876920
http://dx.doi.org/10.1155/2021/1001446
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