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β-hydroxybutyrate Alleviates Learning and Memory Impairment Through the SIRT1 Pathway in D-Galactose-Injured Mice

Learning and memory impairment is a common clinical symptom of aging and nervous system injuries, and seriously affects quality of life. Memory impairment is associated with increased oxidative stress (OS) and inflammatory response. β-hydroxybutyrate (BHBA) is a water-soluble endogenous small-molecu...

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Autores principales: Yang, Xiaojing, Wang, Ruonan, Zhou, Hailun, Wang, Li, Wang, Rui, Li, Haomin, Tan, Baodong, Wu, Qiong, Xu, Xin, Cui, Lianxu, Li, Zaiyu, Li, Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8645592/
https://www.ncbi.nlm.nih.gov/pubmed/34880753
http://dx.doi.org/10.3389/fphar.2021.751028
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author Yang, Xiaojing
Wang, Ruonan
Zhou, Hailun
Wang, Li
Wang, Rui
Li, Haomin
Tan, Baodong
Wu, Qiong
Xu, Xin
Cui, Lianxu
Li, Zaiyu
Li, Hua
author_facet Yang, Xiaojing
Wang, Ruonan
Zhou, Hailun
Wang, Li
Wang, Rui
Li, Haomin
Tan, Baodong
Wu, Qiong
Xu, Xin
Cui, Lianxu
Li, Zaiyu
Li, Hua
author_sort Yang, Xiaojing
collection PubMed
description Learning and memory impairment is a common clinical symptom of aging and nervous system injuries, and seriously affects quality of life. Memory impairment is associated with increased oxidative stress (OS) and inflammatory response. β-hydroxybutyrate (BHBA) is a water-soluble endogenous small-molecule ketone body that easily crosses the blood-brain barrier and has shown neuroprotection activities. In this study, we investigated the effects and mechanisms of BHBA on D-galactose (D-gal)-induced memory impairment in mice by in vitro and in vivo experiments. BHBA was administered intragastrically to D-gal-injured C57BL/6 mice for 42 days. Water maze performance, the morphology of the hippocampus with Nissl staining, the ACh content, OS, and inflammation status were examined. To further investigate the mechanism, hippocampal neuronal cells (HT22) were treated with BHBA with or without the SIRT1 inhibitor or small interfering RNAs against sirt1 (si-SIRT1) before incubation with D-gal. BHBA significantly improved water maze performance; increased the ACh content, SOD activity, and SIRT1 expression; and decreased AChE and LDH activity, ROS, MDA, IL-1β, TNF-α contents, and NLRP3 expression. Further studies with the SIRT inhibitor or siRNAs against sirt1 reversed the above effects of BHBA. Collectively, BHBA inhibited hippocampal OS and the inflammation process to alleviate learning and memory impairment through activating the SIRT1 pathway in D-gal-injured mice, suggesting that BHBA could be a potential option for drug development of learning and memory impairment induced by nervous system injuries.
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spelling pubmed-86455922021-12-07 β-hydroxybutyrate Alleviates Learning and Memory Impairment Through the SIRT1 Pathway in D-Galactose-Injured Mice Yang, Xiaojing Wang, Ruonan Zhou, Hailun Wang, Li Wang, Rui Li, Haomin Tan, Baodong Wu, Qiong Xu, Xin Cui, Lianxu Li, Zaiyu Li, Hua Front Pharmacol Pharmacology Learning and memory impairment is a common clinical symptom of aging and nervous system injuries, and seriously affects quality of life. Memory impairment is associated with increased oxidative stress (OS) and inflammatory response. β-hydroxybutyrate (BHBA) is a water-soluble endogenous small-molecule ketone body that easily crosses the blood-brain barrier and has shown neuroprotection activities. In this study, we investigated the effects and mechanisms of BHBA on D-galactose (D-gal)-induced memory impairment in mice by in vitro and in vivo experiments. BHBA was administered intragastrically to D-gal-injured C57BL/6 mice for 42 days. Water maze performance, the morphology of the hippocampus with Nissl staining, the ACh content, OS, and inflammation status were examined. To further investigate the mechanism, hippocampal neuronal cells (HT22) were treated with BHBA with or without the SIRT1 inhibitor or small interfering RNAs against sirt1 (si-SIRT1) before incubation with D-gal. BHBA significantly improved water maze performance; increased the ACh content, SOD activity, and SIRT1 expression; and decreased AChE and LDH activity, ROS, MDA, IL-1β, TNF-α contents, and NLRP3 expression. Further studies with the SIRT inhibitor or siRNAs against sirt1 reversed the above effects of BHBA. Collectively, BHBA inhibited hippocampal OS and the inflammation process to alleviate learning and memory impairment through activating the SIRT1 pathway in D-gal-injured mice, suggesting that BHBA could be a potential option for drug development of learning and memory impairment induced by nervous system injuries. Frontiers Media S.A. 2021-11-22 /pmc/articles/PMC8645592/ /pubmed/34880753 http://dx.doi.org/10.3389/fphar.2021.751028 Text en Copyright © 2021 Yang, Wang, Zhou, Wang, Wang, Li, Tan, Wu, Xu, Cui, Li and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Yang, Xiaojing
Wang, Ruonan
Zhou, Hailun
Wang, Li
Wang, Rui
Li, Haomin
Tan, Baodong
Wu, Qiong
Xu, Xin
Cui, Lianxu
Li, Zaiyu
Li, Hua
β-hydroxybutyrate Alleviates Learning and Memory Impairment Through the SIRT1 Pathway in D-Galactose-Injured Mice
title β-hydroxybutyrate Alleviates Learning and Memory Impairment Through the SIRT1 Pathway in D-Galactose-Injured Mice
title_full β-hydroxybutyrate Alleviates Learning and Memory Impairment Through the SIRT1 Pathway in D-Galactose-Injured Mice
title_fullStr β-hydroxybutyrate Alleviates Learning and Memory Impairment Through the SIRT1 Pathway in D-Galactose-Injured Mice
title_full_unstemmed β-hydroxybutyrate Alleviates Learning and Memory Impairment Through the SIRT1 Pathway in D-Galactose-Injured Mice
title_short β-hydroxybutyrate Alleviates Learning and Memory Impairment Through the SIRT1 Pathway in D-Galactose-Injured Mice
title_sort β-hydroxybutyrate alleviates learning and memory impairment through the sirt1 pathway in d-galactose-injured mice
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8645592/
https://www.ncbi.nlm.nih.gov/pubmed/34880753
http://dx.doi.org/10.3389/fphar.2021.751028
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