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A Severe Gastroenteritis Outbreak of Salmonella enterica Serovar Enteritidis Linked to Contaminated Egg Fried Rice, China, 2021

Salmonella contamination of eggs and egg shells has been identified as a public health problem worldwide. Here, we reported an outbreak of severe gastrointestinal symptoms caused by Salmonella enterica serovar Enteritidis (S. enteritidis) in China. We evaluated the outbreak by using epidemiological...

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Autores principales: Zhang, Yaowen, Liu, Kangkang, Zhang, Zhenbiao, Tian, Sai, Liu, Xiong, Qi, Hongjuan, Dong, Derong, Wang, Yong, Liu, Meiling, Li, Xinge, Han, Yiran, Zhu, Kunpeng, Liu, Hongbo, Yang, Chaojie, Liu, HONGBO, Du, Xinying, Wang, Qi, Wang, Hui, Yang, Mingjuan, Wang, Ligui, Song, Hongbin, Yang, Haiyan, Xiang, Ying, Qiu, Shaofu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8645860/
https://www.ncbi.nlm.nih.gov/pubmed/34880847
http://dx.doi.org/10.3389/fmicb.2021.779749
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author Zhang, Yaowen
Liu, Kangkang
Zhang, Zhenbiao
Tian, Sai
Liu, Xiong
Qi, Hongjuan
Dong, Derong
Wang, Yong
Liu, Meiling
Li, Xinge
Han, Yiran
Zhu, Kunpeng
Liu, Hongbo
Yang, Chaojie
Liu, HONGBO
Du, Xinying
Wang, Qi
Wang, Hui
Yang, Mingjuan
Wang, Ligui
Song, Hongbin
Yang, Haiyan
Xiang, Ying
Qiu, Shaofu
author_facet Zhang, Yaowen
Liu, Kangkang
Zhang, Zhenbiao
Tian, Sai
Liu, Xiong
Qi, Hongjuan
Dong, Derong
Wang, Yong
Liu, Meiling
Li, Xinge
Han, Yiran
Zhu, Kunpeng
Liu, Hongbo
Yang, Chaojie
Liu, HONGBO
Du, Xinying
Wang, Qi
Wang, Hui
Yang, Mingjuan
Wang, Ligui
Song, Hongbin
Yang, Haiyan
Xiang, Ying
Qiu, Shaofu
author_sort Zhang, Yaowen
collection PubMed
description Salmonella contamination of eggs and egg shells has been identified as a public health problem worldwide. Here, we reported an outbreak of severe gastrointestinal symptoms caused by Salmonella enterica serovar Enteritidis (S. enteritidis) in China. We evaluated the outbreak by using epidemiological surveys, routine laboratory testing methods, and whole genome sequencing (WGS). This outbreak occurred in a canteen in Beijing, during March 9–11, 2021, 225 of the 324 diners who have eaten at the canteen showed gastrointestinal symptoms. The outbreak had characteristical epidemiological and clinical features. It caused a very high attack rate (69.4%) in a short incubation time. All patients developed diarrhea and high fever, accompanied by abdominal pain (62.3%), nausea (50.4%), and vomiting (62.7%). The average frequency of diarrhea was 12.4 times/day, and the highest frequency of diarrhea was as high as 50 times/day. The average fever temperature was 39.4°C, and the highest fever temperature was 42°C. Twenty strains of S. enteritidis were recovered, including 19 from the patients samples, and one from remained egg fried rice. Antibiotic susceptibility test showed that the 20 outbreak strains all had the same resistance pattern. PFGE results demonstrated that all 20 strains bore completely identical bands. Phylogenetic analysis based on WGS revealed that all 20 outbreak strains were tightly clustered together. So the pathogenic source of this food poisoning incident may was contaminated egg fried rice. Resistance gene analysis showed that the outbreak strains are all multi-drug resistant strains. Virulence gene analysis indicated that these outbreak strains carried a large number of virulence genes, including 2 types of Salmonella pathogenicity islands (SPI-1 and SPI-2). Other important virulence genes were also carried by the outbreak strains, such as pefABCD, rck and shdA. And the shdA gene was not in other strains located in the same evolutionary branch as the outbreak strain. We speculated that this is a significant reason for the serious symptoms of gastroenteritis in this outbreak. This outbreak caused by S. enteritidis suggested government should strengthen monitoring of the prevalence of outbreak clone strains, and take measures to mitigate the public health threat posed by contaminated eggs.
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spelling pubmed-86458602021-12-07 A Severe Gastroenteritis Outbreak of Salmonella enterica Serovar Enteritidis Linked to Contaminated Egg Fried Rice, China, 2021 Zhang, Yaowen Liu, Kangkang Zhang, Zhenbiao Tian, Sai Liu, Xiong Qi, Hongjuan Dong, Derong Wang, Yong Liu, Meiling Li, Xinge Han, Yiran Zhu, Kunpeng Liu, Hongbo Yang, Chaojie Liu, HONGBO Du, Xinying Wang, Qi Wang, Hui Yang, Mingjuan Wang, Ligui Song, Hongbin Yang, Haiyan Xiang, Ying Qiu, Shaofu Front Microbiol Microbiology Salmonella contamination of eggs and egg shells has been identified as a public health problem worldwide. Here, we reported an outbreak of severe gastrointestinal symptoms caused by Salmonella enterica serovar Enteritidis (S. enteritidis) in China. We evaluated the outbreak by using epidemiological surveys, routine laboratory testing methods, and whole genome sequencing (WGS). This outbreak occurred in a canteen in Beijing, during March 9–11, 2021, 225 of the 324 diners who have eaten at the canteen showed gastrointestinal symptoms. The outbreak had characteristical epidemiological and clinical features. It caused a very high attack rate (69.4%) in a short incubation time. All patients developed diarrhea and high fever, accompanied by abdominal pain (62.3%), nausea (50.4%), and vomiting (62.7%). The average frequency of diarrhea was 12.4 times/day, and the highest frequency of diarrhea was as high as 50 times/day. The average fever temperature was 39.4°C, and the highest fever temperature was 42°C. Twenty strains of S. enteritidis were recovered, including 19 from the patients samples, and one from remained egg fried rice. Antibiotic susceptibility test showed that the 20 outbreak strains all had the same resistance pattern. PFGE results demonstrated that all 20 strains bore completely identical bands. Phylogenetic analysis based on WGS revealed that all 20 outbreak strains were tightly clustered together. So the pathogenic source of this food poisoning incident may was contaminated egg fried rice. Resistance gene analysis showed that the outbreak strains are all multi-drug resistant strains. Virulence gene analysis indicated that these outbreak strains carried a large number of virulence genes, including 2 types of Salmonella pathogenicity islands (SPI-1 and SPI-2). Other important virulence genes were also carried by the outbreak strains, such as pefABCD, rck and shdA. And the shdA gene was not in other strains located in the same evolutionary branch as the outbreak strain. We speculated that this is a significant reason for the serious symptoms of gastroenteritis in this outbreak. This outbreak caused by S. enteritidis suggested government should strengthen monitoring of the prevalence of outbreak clone strains, and take measures to mitigate the public health threat posed by contaminated eggs. Frontiers Media S.A. 2021-11-22 /pmc/articles/PMC8645860/ /pubmed/34880847 http://dx.doi.org/10.3389/fmicb.2021.779749 Text en Copyright © 2021 Zhang, Liu, Zhang, Tian, Liu, Qi, Dong, Wang, Liu, Li, Han, Zhu, Liu, Yang, Liu, Du, Wang, Wang, Yang, Wang, Song, Yang, Xiang and Qiu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhang, Yaowen
Liu, Kangkang
Zhang, Zhenbiao
Tian, Sai
Liu, Xiong
Qi, Hongjuan
Dong, Derong
Wang, Yong
Liu, Meiling
Li, Xinge
Han, Yiran
Zhu, Kunpeng
Liu, Hongbo
Yang, Chaojie
Liu, HONGBO
Du, Xinying
Wang, Qi
Wang, Hui
Yang, Mingjuan
Wang, Ligui
Song, Hongbin
Yang, Haiyan
Xiang, Ying
Qiu, Shaofu
A Severe Gastroenteritis Outbreak of Salmonella enterica Serovar Enteritidis Linked to Contaminated Egg Fried Rice, China, 2021
title A Severe Gastroenteritis Outbreak of Salmonella enterica Serovar Enteritidis Linked to Contaminated Egg Fried Rice, China, 2021
title_full A Severe Gastroenteritis Outbreak of Salmonella enterica Serovar Enteritidis Linked to Contaminated Egg Fried Rice, China, 2021
title_fullStr A Severe Gastroenteritis Outbreak of Salmonella enterica Serovar Enteritidis Linked to Contaminated Egg Fried Rice, China, 2021
title_full_unstemmed A Severe Gastroenteritis Outbreak of Salmonella enterica Serovar Enteritidis Linked to Contaminated Egg Fried Rice, China, 2021
title_short A Severe Gastroenteritis Outbreak of Salmonella enterica Serovar Enteritidis Linked to Contaminated Egg Fried Rice, China, 2021
title_sort severe gastroenteritis outbreak of salmonella enterica serovar enteritidis linked to contaminated egg fried rice, china, 2021
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8645860/
https://www.ncbi.nlm.nih.gov/pubmed/34880847
http://dx.doi.org/10.3389/fmicb.2021.779749
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