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Effect of Co-exposure to Heat and Psychological Stressors on Sperm DNA and Semen Parameters

The present study aims to investigate the effects of co-exposure to heat and psychological stress on sperm DNA and semen parameters among male rats. The study was conducted on 40 healthy adult male Wistar rats. The rats were randomly categorized into four groups of same size consisting of a control...

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Autores principales: Abdollahi, Farnaz, Farhang Dehghan, Somayeh, Amanpour, Saeid, Haghparast, Abbas, Sabour, Siamak, Zendehdel, Rezvan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8646159/
https://www.ncbi.nlm.nih.gov/pubmed/34917486
http://dx.doi.org/10.1016/j.toxrep.2021.11.015
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author Abdollahi, Farnaz
Farhang Dehghan, Somayeh
Amanpour, Saeid
Haghparast, Abbas
Sabour, Siamak
Zendehdel, Rezvan
author_facet Abdollahi, Farnaz
Farhang Dehghan, Somayeh
Amanpour, Saeid
Haghparast, Abbas
Sabour, Siamak
Zendehdel, Rezvan
author_sort Abdollahi, Farnaz
collection PubMed
description The present study aims to investigate the effects of co-exposure to heat and psychological stress on sperm DNA and semen parameters among male rats. The study was conducted on 40 healthy adult male Wistar rats. The rats were randomly categorized into four groups of same size consisting of a control group, a heat stress, psychological and co-exposure groups. The heat stress group was exposed to a temperature of 36 °C at 20% relative humidity. The psychological stress exposure group was subjected to three stressors including exposure to strobe light, noise and tilting cage. According the results,the co-exposure group had lower mean sperm parameters including sperm count (17.22 ± 4.22 10(6)/ml), motility (42.63 ± 12.95 %), viability (48.50 ± 23.25 %), normal morphology (56 ± 7.5%), progressive motility (11.61 ± 7.81%), non-progressive motility (31.18 ± 7.77%), curvilinear velocity (24.11 ± 3.81 μm/s) and straight-line velocity (3.2 ± 1.4 μm/s) when compared with those of the other groups (P = 0.001). Mean sperm immobility (57.36 ± 12.95%) and non-progressive motility (37.93 ± 11.15%) in the co-exposure group was higher compared to the other groups (P = 0.001 and P = 0.333, respectively). Assessment of damage to sperm DNA revealed that the heat exposure group had a higher percentage of sperm DNA damage (9.44 ± 6.80 %) compared to others (P = 0.185). In case of all of exposure scenario, the chance that the semen quality decreased compared to the control group has been increased. In general the combined stress had a greater significant effect on sperm parameters compared to other exposure groups, except for DNA damage.
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spelling pubmed-86461592021-12-15 Effect of Co-exposure to Heat and Psychological Stressors on Sperm DNA and Semen Parameters Abdollahi, Farnaz Farhang Dehghan, Somayeh Amanpour, Saeid Haghparast, Abbas Sabour, Siamak Zendehdel, Rezvan Toxicol Rep Regular Article The present study aims to investigate the effects of co-exposure to heat and psychological stress on sperm DNA and semen parameters among male rats. The study was conducted on 40 healthy adult male Wistar rats. The rats were randomly categorized into four groups of same size consisting of a control group, a heat stress, psychological and co-exposure groups. The heat stress group was exposed to a temperature of 36 °C at 20% relative humidity. The psychological stress exposure group was subjected to three stressors including exposure to strobe light, noise and tilting cage. According the results,the co-exposure group had lower mean sperm parameters including sperm count (17.22 ± 4.22 10(6)/ml), motility (42.63 ± 12.95 %), viability (48.50 ± 23.25 %), normal morphology (56 ± 7.5%), progressive motility (11.61 ± 7.81%), non-progressive motility (31.18 ± 7.77%), curvilinear velocity (24.11 ± 3.81 μm/s) and straight-line velocity (3.2 ± 1.4 μm/s) when compared with those of the other groups (P = 0.001). Mean sperm immobility (57.36 ± 12.95%) and non-progressive motility (37.93 ± 11.15%) in the co-exposure group was higher compared to the other groups (P = 0.001 and P = 0.333, respectively). Assessment of damage to sperm DNA revealed that the heat exposure group had a higher percentage of sperm DNA damage (9.44 ± 6.80 %) compared to others (P = 0.185). In case of all of exposure scenario, the chance that the semen quality decreased compared to the control group has been increased. In general the combined stress had a greater significant effect on sperm parameters compared to other exposure groups, except for DNA damage. Elsevier 2021-11-25 /pmc/articles/PMC8646159/ /pubmed/34917486 http://dx.doi.org/10.1016/j.toxrep.2021.11.015 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Regular Article
Abdollahi, Farnaz
Farhang Dehghan, Somayeh
Amanpour, Saeid
Haghparast, Abbas
Sabour, Siamak
Zendehdel, Rezvan
Effect of Co-exposure to Heat and Psychological Stressors on Sperm DNA and Semen Parameters
title Effect of Co-exposure to Heat and Psychological Stressors on Sperm DNA and Semen Parameters
title_full Effect of Co-exposure to Heat and Psychological Stressors on Sperm DNA and Semen Parameters
title_fullStr Effect of Co-exposure to Heat and Psychological Stressors on Sperm DNA and Semen Parameters
title_full_unstemmed Effect of Co-exposure to Heat and Psychological Stressors on Sperm DNA and Semen Parameters
title_short Effect of Co-exposure to Heat and Psychological Stressors on Sperm DNA and Semen Parameters
title_sort effect of co-exposure to heat and psychological stressors on sperm dna and semen parameters
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8646159/
https://www.ncbi.nlm.nih.gov/pubmed/34917486
http://dx.doi.org/10.1016/j.toxrep.2021.11.015
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