A macrophage-T cell coculture model for severe tissue injury-induced T cell death

A key observation of tissue injury, such as stroke and burn, is a state of systemic immunosuppression characterized by loss of T cells and rise of infections. Here, we present an in vitro model for cell-cell interactions between innate (macrophages) and adaptive (T cells) immune cells. This protocol...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, Jie, Cao, Jiayu, Liesz, Arthur, Roth, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8646263/
https://www.ncbi.nlm.nih.gov/pubmed/34927092
http://dx.doi.org/10.1016/j.xpro.2021.100983
_version_ 1784610482674466816
author Zhu, Jie
Cao, Jiayu
Liesz, Arthur
Roth, Stefan
author_facet Zhu, Jie
Cao, Jiayu
Liesz, Arthur
Roth, Stefan
author_sort Zhu, Jie
collection PubMed
description A key observation of tissue injury, such as stroke and burn, is a state of systemic immunosuppression characterized by loss of T cells and rise of infections. Here, we present an in vitro model for cell-cell interactions between innate (macrophages) and adaptive (T cells) immune cells. This protocol facilitates bone marrow-derived macrophages (BMDMs) and splenic T cells in a coculture model. The procedure mimics injury-induced T cell death, which is driven by inflammasome activation in macrophages. For complete details on the use and execution of this protocol, please refer to Roth et al. (2021).
format Online
Article
Text
id pubmed-8646263
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-86462632021-12-17 A macrophage-T cell coculture model for severe tissue injury-induced T cell death Zhu, Jie Cao, Jiayu Liesz, Arthur Roth, Stefan STAR Protoc Protocol A key observation of tissue injury, such as stroke and burn, is a state of systemic immunosuppression characterized by loss of T cells and rise of infections. Here, we present an in vitro model for cell-cell interactions between innate (macrophages) and adaptive (T cells) immune cells. This protocol facilitates bone marrow-derived macrophages (BMDMs) and splenic T cells in a coculture model. The procedure mimics injury-induced T cell death, which is driven by inflammasome activation in macrophages. For complete details on the use and execution of this protocol, please refer to Roth et al. (2021). Elsevier 2021-12-01 /pmc/articles/PMC8646263/ /pubmed/34927092 http://dx.doi.org/10.1016/j.xpro.2021.100983 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Zhu, Jie
Cao, Jiayu
Liesz, Arthur
Roth, Stefan
A macrophage-T cell coculture model for severe tissue injury-induced T cell death
title A macrophage-T cell coculture model for severe tissue injury-induced T cell death
title_full A macrophage-T cell coculture model for severe tissue injury-induced T cell death
title_fullStr A macrophage-T cell coculture model for severe tissue injury-induced T cell death
title_full_unstemmed A macrophage-T cell coculture model for severe tissue injury-induced T cell death
title_short A macrophage-T cell coculture model for severe tissue injury-induced T cell death
title_sort macrophage-t cell coculture model for severe tissue injury-induced t cell death
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8646263/
https://www.ncbi.nlm.nih.gov/pubmed/34927092
http://dx.doi.org/10.1016/j.xpro.2021.100983
work_keys_str_mv AT zhujie amacrophagetcellcoculturemodelforseveretissueinjuryinducedtcelldeath
AT caojiayu amacrophagetcellcoculturemodelforseveretissueinjuryinducedtcelldeath
AT lieszarthur amacrophagetcellcoculturemodelforseveretissueinjuryinducedtcelldeath
AT rothstefan amacrophagetcellcoculturemodelforseveretissueinjuryinducedtcelldeath
AT zhujie macrophagetcellcoculturemodelforseveretissueinjuryinducedtcelldeath
AT caojiayu macrophagetcellcoculturemodelforseveretissueinjuryinducedtcelldeath
AT lieszarthur macrophagetcellcoculturemodelforseveretissueinjuryinducedtcelldeath
AT rothstefan macrophagetcellcoculturemodelforseveretissueinjuryinducedtcelldeath

Ejemplares similares