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Immobilization of Glycogen Synthase Kinase-3β Inhibitor on 316L Stainless Steel via Polydopamine to Accelerate Endothelialization

The coverage of stents with healthy endothelium is crucial to the success of cardiovascular stent implantation. Immobilizing bioactive molecules on stents is an effective strategy to generate such stents. Glycogen synthase kinase-3β inhibitor (GSKi) is a bioactive molecule that can effectively accel...

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Autores principales: Zhang, Ming, Shi, Xudong, Sun, Hai, Xu, Donghua, Gao, Yang, Wu, Xi, Zhang, Jianqi, Zhang, Jichang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8646698/
https://www.ncbi.nlm.nih.gov/pubmed/34881239
http://dx.doi.org/10.3389/fbioe.2021.806151
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author Zhang, Ming
Shi, Xudong
Sun, Hai
Xu, Donghua
Gao, Yang
Wu, Xi
Zhang, Jianqi
Zhang, Jichang
author_facet Zhang, Ming
Shi, Xudong
Sun, Hai
Xu, Donghua
Gao, Yang
Wu, Xi
Zhang, Jianqi
Zhang, Jichang
author_sort Zhang, Ming
collection PubMed
description The coverage of stents with healthy endothelium is crucial to the success of cardiovascular stent implantation. Immobilizing bioactive molecules on stents is an effective strategy to generate such stents. Glycogen synthase kinase-3β inhibitor (GSKi) is a bioactive molecule that can effectively accelerate vascular endothelialization. In this work, GSKi was covalently conjugated on 316L stainless steel through polydopamine to develop a stable bioactive surface. Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and water contact angle results revealed the successful introduction of GSKi onto 316L stainless steel. The GSKi coating did not obviously affect the hemocompatibility of plates. The adhesion and proliferation of human coronary artery endothelial cells (HCAECs) on stainless steel was significantly promoted by the addition of GSKi. In summary, this work provides a universal and stable strategy of immobilizing GSKi on the stent surface. This method has the potential for widespread application in the modification of vascular stents.
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spelling pubmed-86466982021-12-07 Immobilization of Glycogen Synthase Kinase-3β Inhibitor on 316L Stainless Steel via Polydopamine to Accelerate Endothelialization Zhang, Ming Shi, Xudong Sun, Hai Xu, Donghua Gao, Yang Wu, Xi Zhang, Jianqi Zhang, Jichang Front Bioeng Biotechnol Bioengineering and Biotechnology The coverage of stents with healthy endothelium is crucial to the success of cardiovascular stent implantation. Immobilizing bioactive molecules on stents is an effective strategy to generate such stents. Glycogen synthase kinase-3β inhibitor (GSKi) is a bioactive molecule that can effectively accelerate vascular endothelialization. In this work, GSKi was covalently conjugated on 316L stainless steel through polydopamine to develop a stable bioactive surface. Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and water contact angle results revealed the successful introduction of GSKi onto 316L stainless steel. The GSKi coating did not obviously affect the hemocompatibility of plates. The adhesion and proliferation of human coronary artery endothelial cells (HCAECs) on stainless steel was significantly promoted by the addition of GSKi. In summary, this work provides a universal and stable strategy of immobilizing GSKi on the stent surface. This method has the potential for widespread application in the modification of vascular stents. Frontiers Media S.A. 2021-11-22 /pmc/articles/PMC8646698/ /pubmed/34881239 http://dx.doi.org/10.3389/fbioe.2021.806151 Text en Copyright © 2021 Zhang, Shi, Sun, Xu, Gao, Wu, Zhang and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Zhang, Ming
Shi, Xudong
Sun, Hai
Xu, Donghua
Gao, Yang
Wu, Xi
Zhang, Jianqi
Zhang, Jichang
Immobilization of Glycogen Synthase Kinase-3β Inhibitor on 316L Stainless Steel via Polydopamine to Accelerate Endothelialization
title Immobilization of Glycogen Synthase Kinase-3β Inhibitor on 316L Stainless Steel via Polydopamine to Accelerate Endothelialization
title_full Immobilization of Glycogen Synthase Kinase-3β Inhibitor on 316L Stainless Steel via Polydopamine to Accelerate Endothelialization
title_fullStr Immobilization of Glycogen Synthase Kinase-3β Inhibitor on 316L Stainless Steel via Polydopamine to Accelerate Endothelialization
title_full_unstemmed Immobilization of Glycogen Synthase Kinase-3β Inhibitor on 316L Stainless Steel via Polydopamine to Accelerate Endothelialization
title_short Immobilization of Glycogen Synthase Kinase-3β Inhibitor on 316L Stainless Steel via Polydopamine to Accelerate Endothelialization
title_sort immobilization of glycogen synthase kinase-3β inhibitor on 316l stainless steel via polydopamine to accelerate endothelialization
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8646698/
https://www.ncbi.nlm.nih.gov/pubmed/34881239
http://dx.doi.org/10.3389/fbioe.2021.806151
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