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The microbiological characteristics of Staphylococcus aureus isolated from patients with native valve infective endocarditis
The microbiological characteristics of Staphylococcus aureus causing infective endocarditis (IE) have not been investigated thoroughly. We compared the characteristics of S. aureus isolates from patients with and without IE. Cases of S. aureus bacteremia (SAB) were collected from 10 hospitals over 7...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8647854/ https://www.ncbi.nlm.nih.gov/pubmed/31718473 http://dx.doi.org/10.1080/21505594.2019.1685631 |
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author | Kim, Chung-Jong Song, Kyoung-Ho Choe, Pyoeng Gyun Park, Wan Beom Kim, Eu Suk Park, Kyoung Un Kim, Nam Joong Park, Kyung-Hwa Kwak, Yee Gyung Cheon, Shinhye Jang, Hee-Chang Kim, Young Keun Lee, Sun Hee Kiem, Sung-Min Lee, Shinwon Kim, Hong Bin Oh, Myoung-don |
author_facet | Kim, Chung-Jong Song, Kyoung-Ho Choe, Pyoeng Gyun Park, Wan Beom Kim, Eu Suk Park, Kyoung Un Kim, Nam Joong Park, Kyung-Hwa Kwak, Yee Gyung Cheon, Shinhye Jang, Hee-Chang Kim, Young Keun Lee, Sun Hee Kiem, Sung-Min Lee, Shinwon Kim, Hong Bin Oh, Myoung-don |
author_sort | Kim, Chung-Jong |
collection | PubMed |
description | The microbiological characteristics of Staphylococcus aureus causing infective endocarditis (IE) have not been investigated thoroughly. We compared the characteristics of S. aureus isolates from patients with and without IE. Cases of S. aureus bacteremia (SAB) were collected from 10 hospitals over 7 years. Cases of native valve IE were matched with non-IE controls according to the following criteria: central-line-associated infection, community-acquired infection, methicillin susceptibility, and if possible, the primary site of infection. Genes coding virulence factors were analyzed using multiplex polymerase chain reactions. Fibrinogen and fibronectin-binding properties were assessed using in vitro binding assays. The fibronectin-binding protein A gene (fnbpA) was sequenced. Of 2,365 cases of SAB, 92 had IE. After matching, 37 pairs of S. aureus isolates from the IE cases and non-IE controls were compared; fnbpA was detected in 91.9% of the IE isolates and 100% of the non-IE isolates (p = 0.24). While the fibrinogen binding ratio was similar (1.07 ± 0.33 vs. 1.08 ± 0.26, p = 0.89), the fibronectin-binding ratio was significantly higher in the IE-group (1.31 ± 0.42 vs. 1.06 ± 0.31, p = 0.01). The proportions of major single-nucleotide polymorphisms in fnbpA were as follows: E652D (2.9% vs. 2.7%), H782Q (65.6% vs. 60.6%), and K786N (65.6% vs. 72.7%). The fibronectin-binding ratio was positively correlated with the number of SNPs present in IE cases (p < 0.001) but not in the non-IE controls (p = 0.124). Fibronectin-binding might play a key role in SAB IE. However, the degree of binding may be mediated by genetic variability between isolates. |
format | Online Article Text |
id | pubmed-8647854 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-86478542021-12-07 The microbiological characteristics of Staphylococcus aureus isolated from patients with native valve infective endocarditis Kim, Chung-Jong Song, Kyoung-Ho Choe, Pyoeng Gyun Park, Wan Beom Kim, Eu Suk Park, Kyoung Un Kim, Nam Joong Park, Kyung-Hwa Kwak, Yee Gyung Cheon, Shinhye Jang, Hee-Chang Kim, Young Keun Lee, Sun Hee Kiem, Sung-Min Lee, Shinwon Kim, Hong Bin Oh, Myoung-don Virulence Research Paper The microbiological characteristics of Staphylococcus aureus causing infective endocarditis (IE) have not been investigated thoroughly. We compared the characteristics of S. aureus isolates from patients with and without IE. Cases of S. aureus bacteremia (SAB) were collected from 10 hospitals over 7 years. Cases of native valve IE were matched with non-IE controls according to the following criteria: central-line-associated infection, community-acquired infection, methicillin susceptibility, and if possible, the primary site of infection. Genes coding virulence factors were analyzed using multiplex polymerase chain reactions. Fibrinogen and fibronectin-binding properties were assessed using in vitro binding assays. The fibronectin-binding protein A gene (fnbpA) was sequenced. Of 2,365 cases of SAB, 92 had IE. After matching, 37 pairs of S. aureus isolates from the IE cases and non-IE controls were compared; fnbpA was detected in 91.9% of the IE isolates and 100% of the non-IE isolates (p = 0.24). While the fibrinogen binding ratio was similar (1.07 ± 0.33 vs. 1.08 ± 0.26, p = 0.89), the fibronectin-binding ratio was significantly higher in the IE-group (1.31 ± 0.42 vs. 1.06 ± 0.31, p = 0.01). The proportions of major single-nucleotide polymorphisms in fnbpA were as follows: E652D (2.9% vs. 2.7%), H782Q (65.6% vs. 60.6%), and K786N (65.6% vs. 72.7%). The fibronectin-binding ratio was positively correlated with the number of SNPs present in IE cases (p < 0.001) but not in the non-IE controls (p = 0.124). Fibronectin-binding might play a key role in SAB IE. However, the degree of binding may be mediated by genetic variability between isolates. Taylor & Francis 2019-11-13 /pmc/articles/PMC8647854/ /pubmed/31718473 http://dx.doi.org/10.1080/21505594.2019.1685631 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Kim, Chung-Jong Song, Kyoung-Ho Choe, Pyoeng Gyun Park, Wan Beom Kim, Eu Suk Park, Kyoung Un Kim, Nam Joong Park, Kyung-Hwa Kwak, Yee Gyung Cheon, Shinhye Jang, Hee-Chang Kim, Young Keun Lee, Sun Hee Kiem, Sung-Min Lee, Shinwon Kim, Hong Bin Oh, Myoung-don The microbiological characteristics of Staphylococcus aureus isolated from patients with native valve infective endocarditis |
title | The microbiological characteristics of Staphylococcus aureus isolated from patients with native valve infective endocarditis |
title_full | The microbiological characteristics of Staphylococcus aureus isolated from patients with native valve infective endocarditis |
title_fullStr | The microbiological characteristics of Staphylococcus aureus isolated from patients with native valve infective endocarditis |
title_full_unstemmed | The microbiological characteristics of Staphylococcus aureus isolated from patients with native valve infective endocarditis |
title_short | The microbiological characteristics of Staphylococcus aureus isolated from patients with native valve infective endocarditis |
title_sort | microbiological characteristics of staphylococcus aureus isolated from patients with native valve infective endocarditis |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8647854/ https://www.ncbi.nlm.nih.gov/pubmed/31718473 http://dx.doi.org/10.1080/21505594.2019.1685631 |
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