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Expression of a CO(2)-permeable aquaporin enhances mesophyll conductance in the C(4) species Setaria viridis

A fundamental limitation of photosynthetic carbon fixation is the availability of CO(2). In C(4) plants, primary carboxylation occurs in mesophyll cytosol, and little is known about the role of CO(2) diffusion in facilitating C(4) photosynthesis. We have examined the expression, localization, and fu...

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Autores principales: Ermakova, Maria, Osborn, Hannah, Groszmann, Michael, Bala, Soumi, Bowerman, Andrew, McGaughey, Samantha, Byrt, Caitlin, Alonso-cantabrana, Hugo, Tyerman, Steve, Furbank, Robert T, Sharwood, Robert E, von Caemmerer, Susanne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8648302/
https://www.ncbi.nlm.nih.gov/pubmed/34842138
http://dx.doi.org/10.7554/eLife.70095
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author Ermakova, Maria
Osborn, Hannah
Groszmann, Michael
Bala, Soumi
Bowerman, Andrew
McGaughey, Samantha
Byrt, Caitlin
Alonso-cantabrana, Hugo
Tyerman, Steve
Furbank, Robert T
Sharwood, Robert E
von Caemmerer, Susanne
author_facet Ermakova, Maria
Osborn, Hannah
Groszmann, Michael
Bala, Soumi
Bowerman, Andrew
McGaughey, Samantha
Byrt, Caitlin
Alonso-cantabrana, Hugo
Tyerman, Steve
Furbank, Robert T
Sharwood, Robert E
von Caemmerer, Susanne
author_sort Ermakova, Maria
collection PubMed
description A fundamental limitation of photosynthetic carbon fixation is the availability of CO(2). In C(4) plants, primary carboxylation occurs in mesophyll cytosol, and little is known about the role of CO(2) diffusion in facilitating C(4) photosynthesis. We have examined the expression, localization, and functional role of selected plasma membrane intrinsic aquaporins (PIPs) from Setaria italica (foxtail millet) and discovered that SiPIP2;7 is CO(2)-permeable. When ectopically expressed in mesophyll cells of Setaria viridis (green foxtail), SiPIP2;7 was localized to the plasma membrane and caused no marked changes in leaf biochemistry. Gas exchange and C(18)O(16)O discrimination measurements revealed that targeted expression of SiPIP2;7 enhanced the conductance to CO(2) diffusion from the intercellular airspace to the mesophyll cytosol. Our results demonstrate that mesophyll conductance limits C(4) photosynthesis at low pCO(2) and that SiPIP2;7 is a functional CO(2) permeable aquaporin that can improve CO(2) diffusion at the airspace/mesophyll interface and enhance C(4) photosynthesis.
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spelling pubmed-86483022021-12-08 Expression of a CO(2)-permeable aquaporin enhances mesophyll conductance in the C(4) species Setaria viridis Ermakova, Maria Osborn, Hannah Groszmann, Michael Bala, Soumi Bowerman, Andrew McGaughey, Samantha Byrt, Caitlin Alonso-cantabrana, Hugo Tyerman, Steve Furbank, Robert T Sharwood, Robert E von Caemmerer, Susanne eLife Plant Biology A fundamental limitation of photosynthetic carbon fixation is the availability of CO(2). In C(4) plants, primary carboxylation occurs in mesophyll cytosol, and little is known about the role of CO(2) diffusion in facilitating C(4) photosynthesis. We have examined the expression, localization, and functional role of selected plasma membrane intrinsic aquaporins (PIPs) from Setaria italica (foxtail millet) and discovered that SiPIP2;7 is CO(2)-permeable. When ectopically expressed in mesophyll cells of Setaria viridis (green foxtail), SiPIP2;7 was localized to the plasma membrane and caused no marked changes in leaf biochemistry. Gas exchange and C(18)O(16)O discrimination measurements revealed that targeted expression of SiPIP2;7 enhanced the conductance to CO(2) diffusion from the intercellular airspace to the mesophyll cytosol. Our results demonstrate that mesophyll conductance limits C(4) photosynthesis at low pCO(2) and that SiPIP2;7 is a functional CO(2) permeable aquaporin that can improve CO(2) diffusion at the airspace/mesophyll interface and enhance C(4) photosynthesis. eLife Sciences Publications, Ltd 2021-11-29 /pmc/articles/PMC8648302/ /pubmed/34842138 http://dx.doi.org/10.7554/eLife.70095 Text en © 2021, Ermakova et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Plant Biology
Ermakova, Maria
Osborn, Hannah
Groszmann, Michael
Bala, Soumi
Bowerman, Andrew
McGaughey, Samantha
Byrt, Caitlin
Alonso-cantabrana, Hugo
Tyerman, Steve
Furbank, Robert T
Sharwood, Robert E
von Caemmerer, Susanne
Expression of a CO(2)-permeable aquaporin enhances mesophyll conductance in the C(4) species Setaria viridis
title Expression of a CO(2)-permeable aquaporin enhances mesophyll conductance in the C(4) species Setaria viridis
title_full Expression of a CO(2)-permeable aquaporin enhances mesophyll conductance in the C(4) species Setaria viridis
title_fullStr Expression of a CO(2)-permeable aquaporin enhances mesophyll conductance in the C(4) species Setaria viridis
title_full_unstemmed Expression of a CO(2)-permeable aquaporin enhances mesophyll conductance in the C(4) species Setaria viridis
title_short Expression of a CO(2)-permeable aquaporin enhances mesophyll conductance in the C(4) species Setaria viridis
title_sort expression of a co(2)-permeable aquaporin enhances mesophyll conductance in the c(4) species setaria viridis
topic Plant Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8648302/
https://www.ncbi.nlm.nih.gov/pubmed/34842138
http://dx.doi.org/10.7554/eLife.70095
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