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Multiplex CRISPR-Cas9 mutagenesis of the phytochrome gene family in Physcomitrium (Physcomitrella) patens

KEY MESSAGE: We mutated all seven Physcomitrium (Physcomitrella) patens phytochrome genes using highly-efficient CRISPR-Cas9 procedures. We thereby identified phy5a as the phytochrome primarily responsible for inhibiting gravitropism, proving the utility of the mutant library. ABSTRACT: The CRISPR-C...

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Detalles Bibliográficos
Autores principales: Trogu, Silvia, Ermert, Anna Lena, Stahl, Fabian, Nogué, Fabien, Gans, Tanja, Hughes, Jon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8648701/
https://www.ncbi.nlm.nih.gov/pubmed/33346897
http://dx.doi.org/10.1007/s11103-020-01103-x

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