Cargando…
Graphene oxide and fluorescent aptamer based novel biosensor for detection of 25-hydroxyvitamin D(3)
For maintaining the healthy metabolic status, vitamin D is a beneficial metabolite stored majorly in its pre-activated form, 25-hydroxyvitamin D(3) (25(OH)D(3)). Due to its important role in bone strengthening, the study was planned to quantify 25(OH)D(3) levels in our blood. Quantification techniqu...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8649066/ https://www.ncbi.nlm.nih.gov/pubmed/34873222 http://dx.doi.org/10.1038/s41598-021-02837-4 |
_version_ | 1784610917359550464 |
---|---|
author | Gupta, Ritika Kaul, Sunaina Singh, Vishal Kumar, Sandeep Singhal, Nitin Kumar |
author_facet | Gupta, Ritika Kaul, Sunaina Singh, Vishal Kumar, Sandeep Singhal, Nitin Kumar |
author_sort | Gupta, Ritika |
collection | PubMed |
description | For maintaining the healthy metabolic status, vitamin D is a beneficial metabolite stored majorly in its pre-activated form, 25-hydroxyvitamin D(3) (25(OH)D(3)). Due to its important role in bone strengthening, the study was planned to quantify 25(OH)D(3) levels in our blood. Quantification techniques for 25(OH)D(3) are costly thus requiring a need for a low cost, and sensitive detection methods. In this work, an economic, and sensitive sensor for the detection of 25(OH)D(3) was developed using aptamer and graphene oxide (GO). Aptamer is an oligonucleotide, sensitive towards its target, whereas, GO with 2D nanosheets provides excellent quenching surface. Aptamer labeled with fluorescein (5’, 6-FAM) is adsorbed by π–π interaction on the GO sheets leading to quenching of the fluorescence due to Förster resonance energy transfer (FRET). However, in the presence of 25(OH)D(3), a major portion of aptamer fluorescence remains unaltered, due to its association with 25(OH)D(3). However, in the absence, aptamer fluorescence gets fully quenched. Fluorescence intensity quenching was monitored using fluorescence spectrophotometer and agarose gel based system. The limit of detection of 25(OH)D(3) by this method was found to be 0.15 µg/mL whereas when GO-COOH was used, limit of detection was improved to 0.075 µg/mL. Therefore, this method could come up as a new sensing method in the field of vitamin D detection. |
format | Online Article Text |
id | pubmed-8649066 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-86490662021-12-08 Graphene oxide and fluorescent aptamer based novel biosensor for detection of 25-hydroxyvitamin D(3) Gupta, Ritika Kaul, Sunaina Singh, Vishal Kumar, Sandeep Singhal, Nitin Kumar Sci Rep Article For maintaining the healthy metabolic status, vitamin D is a beneficial metabolite stored majorly in its pre-activated form, 25-hydroxyvitamin D(3) (25(OH)D(3)). Due to its important role in bone strengthening, the study was planned to quantify 25(OH)D(3) levels in our blood. Quantification techniques for 25(OH)D(3) are costly thus requiring a need for a low cost, and sensitive detection methods. In this work, an economic, and sensitive sensor for the detection of 25(OH)D(3) was developed using aptamer and graphene oxide (GO). Aptamer is an oligonucleotide, sensitive towards its target, whereas, GO with 2D nanosheets provides excellent quenching surface. Aptamer labeled with fluorescein (5’, 6-FAM) is adsorbed by π–π interaction on the GO sheets leading to quenching of the fluorescence due to Förster resonance energy transfer (FRET). However, in the presence of 25(OH)D(3), a major portion of aptamer fluorescence remains unaltered, due to its association with 25(OH)D(3). However, in the absence, aptamer fluorescence gets fully quenched. Fluorescence intensity quenching was monitored using fluorescence spectrophotometer and agarose gel based system. The limit of detection of 25(OH)D(3) by this method was found to be 0.15 µg/mL whereas when GO-COOH was used, limit of detection was improved to 0.075 µg/mL. Therefore, this method could come up as a new sensing method in the field of vitamin D detection. Nature Publishing Group UK 2021-12-06 /pmc/articles/PMC8649066/ /pubmed/34873222 http://dx.doi.org/10.1038/s41598-021-02837-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Gupta, Ritika Kaul, Sunaina Singh, Vishal Kumar, Sandeep Singhal, Nitin Kumar Graphene oxide and fluorescent aptamer based novel biosensor for detection of 25-hydroxyvitamin D(3) |
title | Graphene oxide and fluorescent aptamer based novel biosensor for detection of 25-hydroxyvitamin D(3) |
title_full | Graphene oxide and fluorescent aptamer based novel biosensor for detection of 25-hydroxyvitamin D(3) |
title_fullStr | Graphene oxide and fluorescent aptamer based novel biosensor for detection of 25-hydroxyvitamin D(3) |
title_full_unstemmed | Graphene oxide and fluorescent aptamer based novel biosensor for detection of 25-hydroxyvitamin D(3) |
title_short | Graphene oxide and fluorescent aptamer based novel biosensor for detection of 25-hydroxyvitamin D(3) |
title_sort | graphene oxide and fluorescent aptamer based novel biosensor for detection of 25-hydroxyvitamin d(3) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8649066/ https://www.ncbi.nlm.nih.gov/pubmed/34873222 http://dx.doi.org/10.1038/s41598-021-02837-4 |
work_keys_str_mv | AT guptaritika grapheneoxideandfluorescentaptamerbasednovelbiosensorfordetectionof25hydroxyvitamind3 AT kaulsunaina grapheneoxideandfluorescentaptamerbasednovelbiosensorfordetectionof25hydroxyvitamind3 AT singhvishal grapheneoxideandfluorescentaptamerbasednovelbiosensorfordetectionof25hydroxyvitamind3 AT kumarsandeep grapheneoxideandfluorescentaptamerbasednovelbiosensorfordetectionof25hydroxyvitamind3 AT singhalnitinkumar grapheneoxideandfluorescentaptamerbasednovelbiosensorfordetectionof25hydroxyvitamind3 |