Antibodies to the N-Terminal Domain of Angiotensin-Converting Enzyme (ACE2) That Block Its Interaction with SARS-CoV-2 S Protein

SARS-CoV-2 is a new coronavirus that is the cause of COVID-19 pandemic. To enter the cell, the virus interacts via its surface S protein with angiotensin-converting enzyme 2 (ACE2), the main entry receptor on the cell membrane. Most of protective antibodies, including those induced by vaccinations,...

Descripción completa

Detalles Bibliográficos
Autores principales: Krut, V. G., Astrakhantseva, I. V., Chuvpilo, S. A., Efimov, G. A., Ambaryan, S. G., Drutskaya, M. S., Nedospasov, S. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pleiades Publishing 2021
Materias:
Biochemistry, Biophysics, and Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8649309/
https://www.ncbi.nlm.nih.gov/pubmed/34874527
http://dx.doi.org/10.1134/S160767292201001X
_version_ 1784610968644354048
author Krut, V. G.
Astrakhantseva, I. V.
Chuvpilo, S. A.
Efimov, G. A.
Ambaryan, S. G.
Drutskaya, M. S.
Nedospasov, S. A.
author_facet Krut, V. G.
Astrakhantseva, I. V.
Chuvpilo, S. A.
Efimov, G. A.
Ambaryan, S. G.
Drutskaya, M. S.
Nedospasov, S. A.
author_sort Krut, V. G.
collection PubMed
description SARS-CoV-2 is a new coronavirus that is the cause of COVID-19 pandemic. To enter the cell, the virus interacts via its surface S protein with angiotensin-converting enzyme 2 (ACE2), the main entry receptor on the cell membrane. Most of protective antibodies, including those induced by vaccinations, target the S protein, preventing its interaction with the ACE2 receptor. We have evaluated an alternative strategy for blocking the S-ACE2 interaction using new antipeptide antibodies to the N-terminus of the ACE2 molecule. These antibodies allow detection of human ACE2 in vitro and ex vivo.
format Online
Article
Text
id pubmed-8649309
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Pleiades Publishing
record_format MEDLINE/PubMed
spelling pubmed-86493092021-12-07 Antibodies to the N-Terminal Domain of Angiotensin-Converting Enzyme (ACE2) That Block Its Interaction with SARS-CoV-2 S Protein Krut, V. G. Astrakhantseva, I. V. Chuvpilo, S. A. Efimov, G. A. Ambaryan, S. G. Drutskaya, M. S. Nedospasov, S. A. Dokl Biochem Biophys Biochemistry, Biophysics, and Molecular Biology SARS-CoV-2 is a new coronavirus that is the cause of COVID-19 pandemic. To enter the cell, the virus interacts via its surface S protein with angiotensin-converting enzyme 2 (ACE2), the main entry receptor on the cell membrane. Most of protective antibodies, including those induced by vaccinations, target the S protein, preventing its interaction with the ACE2 receptor. We have evaluated an alternative strategy for blocking the S-ACE2 interaction using new antipeptide antibodies to the N-terminus of the ACE2 molecule. These antibodies allow detection of human ACE2 in vitro and ex vivo. Pleiades Publishing 2021-12-04 2022 /pmc/articles/PMC8649309/ /pubmed/34874527 http://dx.doi.org/10.1134/S160767292201001X Text en © Pleiades Publishing, Ltd. 2022, ISSN 1607-6729, Doklady Biochemistry and Biophysics, 2022, Vol. 502, pp. 1–4. © Pleiades Publishing, Ltd., 2022.ISSN 1607-6729, Doklady Biochemistry and Biophysics, 2022. © Pleiades Publishing, Ltd., 2022.Russian Text © The Author(s), 2022, published in Doklady Rossiiskoi Akademii Nauk. Nauki o Zhizni, 2022, Vol. 502, pp. 5–9. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Biochemistry, Biophysics, and Molecular Biology
Krut, V. G.
Astrakhantseva, I. V.
Chuvpilo, S. A.
Efimov, G. A.
Ambaryan, S. G.
Drutskaya, M. S.
Nedospasov, S. A.
Antibodies to the N-Terminal Domain of Angiotensin-Converting Enzyme (ACE2) That Block Its Interaction with SARS-CoV-2 S Protein
title Antibodies to the N-Terminal Domain of Angiotensin-Converting Enzyme (ACE2) That Block Its Interaction with SARS-CoV-2 S Protein
title_full Antibodies to the N-Terminal Domain of Angiotensin-Converting Enzyme (ACE2) That Block Its Interaction with SARS-CoV-2 S Protein
title_fullStr Antibodies to the N-Terminal Domain of Angiotensin-Converting Enzyme (ACE2) That Block Its Interaction with SARS-CoV-2 S Protein
title_full_unstemmed Antibodies to the N-Terminal Domain of Angiotensin-Converting Enzyme (ACE2) That Block Its Interaction with SARS-CoV-2 S Protein
title_short Antibodies to the N-Terminal Domain of Angiotensin-Converting Enzyme (ACE2) That Block Its Interaction with SARS-CoV-2 S Protein
title_sort antibodies to the n-terminal domain of angiotensin-converting enzyme (ace2) that block its interaction with sars-cov-2 s protein
topic Biochemistry, Biophysics, and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8649309/
https://www.ncbi.nlm.nih.gov/pubmed/34874527
http://dx.doi.org/10.1134/S160767292201001X
work_keys_str_mv AT krutvg antibodiestothenterminaldomainofangiotensinconvertingenzymeace2thatblockitsinteractionwithsarscov2sprotein
AT astrakhantsevaiv antibodiestothenterminaldomainofangiotensinconvertingenzymeace2thatblockitsinteractionwithsarscov2sprotein
AT chuvpilosa antibodiestothenterminaldomainofangiotensinconvertingenzymeace2thatblockitsinteractionwithsarscov2sprotein
AT efimovga antibodiestothenterminaldomainofangiotensinconvertingenzymeace2thatblockitsinteractionwithsarscov2sprotein
AT ambaryansg antibodiestothenterminaldomainofangiotensinconvertingenzymeace2thatblockitsinteractionwithsarscov2sprotein
AT drutskayams antibodiestothenterminaldomainofangiotensinconvertingenzymeace2thatblockitsinteractionwithsarscov2sprotein
AT nedospasovsa antibodiestothenterminaldomainofangiotensinconvertingenzymeace2thatblockitsinteractionwithsarscov2sprotein

Ejemplares similares