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Poroelastic osmoregulation of living cell volume
Cells maintain their volume through fine intracellular osmolarity regulation. Osmotic challenges drive fluid into or out of cells causing swelling or shrinkage, respectively. The dynamics of cell volume changes depending on the rheology of the cellular constituents and on how fast the fluid permeate...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8649806/ https://www.ncbi.nlm.nih.gov/pubmed/34927026 http://dx.doi.org/10.1016/j.isci.2021.103482 |
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author | Esteki, Mohammad Hadi Malandrino, Andrea Alemrajabi, Ali Akbar Sheridan, Graham K. Charras, Guillaume Moeendarbary, Emad |
author_facet | Esteki, Mohammad Hadi Malandrino, Andrea Alemrajabi, Ali Akbar Sheridan, Graham K. Charras, Guillaume Moeendarbary, Emad |
author_sort | Esteki, Mohammad Hadi |
collection | PubMed |
description | Cells maintain their volume through fine intracellular osmolarity regulation. Osmotic challenges drive fluid into or out of cells causing swelling or shrinkage, respectively. The dynamics of cell volume changes depending on the rheology of the cellular constituents and on how fast the fluid permeates through the membrane and cytoplasm. We investigated whether and how poroelasticity can describe volume dynamics in response to osmotic shocks. We exposed cells to osmotic perturbations and used defocusing epifluorescence microscopy on membrane-attached fluorescent nanospheres to track volume dynamics with high spatiotemporal resolution. We found that a poroelastic model that considers both geometrical and pressurization rates captures fluid-cytoskeleton interactions, which are rate-limiting factors in controlling volume changes at short timescales. Linking cellular responses to osmotic shocks and cell mechanics through poroelasticity can predict the cell state in health, disease, or in response to novel therapeutics. |
format | Online Article Text |
id | pubmed-8649806 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-86498062021-12-17 Poroelastic osmoregulation of living cell volume Esteki, Mohammad Hadi Malandrino, Andrea Alemrajabi, Ali Akbar Sheridan, Graham K. Charras, Guillaume Moeendarbary, Emad iScience Article Cells maintain their volume through fine intracellular osmolarity regulation. Osmotic challenges drive fluid into or out of cells causing swelling or shrinkage, respectively. The dynamics of cell volume changes depending on the rheology of the cellular constituents and on how fast the fluid permeates through the membrane and cytoplasm. We investigated whether and how poroelasticity can describe volume dynamics in response to osmotic shocks. We exposed cells to osmotic perturbations and used defocusing epifluorescence microscopy on membrane-attached fluorescent nanospheres to track volume dynamics with high spatiotemporal resolution. We found that a poroelastic model that considers both geometrical and pressurization rates captures fluid-cytoskeleton interactions, which are rate-limiting factors in controlling volume changes at short timescales. Linking cellular responses to osmotic shocks and cell mechanics through poroelasticity can predict the cell state in health, disease, or in response to novel therapeutics. Elsevier 2021-11-22 /pmc/articles/PMC8649806/ /pubmed/34927026 http://dx.doi.org/10.1016/j.isci.2021.103482 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Esteki, Mohammad Hadi Malandrino, Andrea Alemrajabi, Ali Akbar Sheridan, Graham K. Charras, Guillaume Moeendarbary, Emad Poroelastic osmoregulation of living cell volume |
title | Poroelastic osmoregulation of living cell volume |
title_full | Poroelastic osmoregulation of living cell volume |
title_fullStr | Poroelastic osmoregulation of living cell volume |
title_full_unstemmed | Poroelastic osmoregulation of living cell volume |
title_short | Poroelastic osmoregulation of living cell volume |
title_sort | poroelastic osmoregulation of living cell volume |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8649806/ https://www.ncbi.nlm.nih.gov/pubmed/34927026 http://dx.doi.org/10.1016/j.isci.2021.103482 |
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