Cargando…
Santin and cirsimaritin from Betula pubescens and Betula pendula buds induce apoptosis in human digestive system cancer cells
Flavonoids are bioactive secondary metabolites of plants, which exert anti‐cancer effects. However, metabolism in enterocytes and the liver can influence the biological activity of flavonoids contained in the diet. Therefore, results from in vitro studies on cancer cells from the digestive tract and...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8650031/ https://www.ncbi.nlm.nih.gov/pubmed/34755444 http://dx.doi.org/10.1111/jcmm.17031 |
_version_ | 1784611122086674432 |
---|---|
author | Szoka, Lukasz Nazaruk, Jolanta Stocki, Marcin Isidorov, Valery |
author_facet | Szoka, Lukasz Nazaruk, Jolanta Stocki, Marcin Isidorov, Valery |
author_sort | Szoka, Lukasz |
collection | PubMed |
description | Flavonoids are bioactive secondary metabolites of plants, which exert anti‐cancer effects. However, metabolism in enterocytes and the liver can influence the biological activity of flavonoids contained in the diet. Therefore, results from in vitro studies on cancer cells from the digestive tract and liver may reflect the real effects in the human body. Previously, we have found that the extract from birch buds exerts antiproliferative activity in a panel of cancer cells. In the present study, the anti‐cancer activity of ten flavonoids isolated from the buds of Betula pubescens and Betula pendula was characterized. Among them, santin and cirsimaritin significantly reduced viability, proliferation and clonogenicity of gastric (AGS), colon (DLD‐1) and liver (HepG2) cancer cells. Both flavonoids induced apoptosis, accompanied by activation of caspase‐3, caspase‐7, caspase‐8 and caspase‐9. Moreover, upregulation of p53 was detected only in wild‐type p53 harbouring cells. Together, our results suggest that santin and cirsimaritin exhibit promising anti‐cancer activity in cultures of digestive system cancer cells. |
format | Online Article Text |
id | pubmed-8650031 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86500312021-12-20 Santin and cirsimaritin from Betula pubescens and Betula pendula buds induce apoptosis in human digestive system cancer cells Szoka, Lukasz Nazaruk, Jolanta Stocki, Marcin Isidorov, Valery J Cell Mol Med Original Articles Flavonoids are bioactive secondary metabolites of plants, which exert anti‐cancer effects. However, metabolism in enterocytes and the liver can influence the biological activity of flavonoids contained in the diet. Therefore, results from in vitro studies on cancer cells from the digestive tract and liver may reflect the real effects in the human body. Previously, we have found that the extract from birch buds exerts antiproliferative activity in a panel of cancer cells. In the present study, the anti‐cancer activity of ten flavonoids isolated from the buds of Betula pubescens and Betula pendula was characterized. Among them, santin and cirsimaritin significantly reduced viability, proliferation and clonogenicity of gastric (AGS), colon (DLD‐1) and liver (HepG2) cancer cells. Both flavonoids induced apoptosis, accompanied by activation of caspase‐3, caspase‐7, caspase‐8 and caspase‐9. Moreover, upregulation of p53 was detected only in wild‐type p53 harbouring cells. Together, our results suggest that santin and cirsimaritin exhibit promising anti‐cancer activity in cultures of digestive system cancer cells. John Wiley and Sons Inc. 2021-11-09 2021-12 /pmc/articles/PMC8650031/ /pubmed/34755444 http://dx.doi.org/10.1111/jcmm.17031 Text en © 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Szoka, Lukasz Nazaruk, Jolanta Stocki, Marcin Isidorov, Valery Santin and cirsimaritin from Betula pubescens and Betula pendula buds induce apoptosis in human digestive system cancer cells |
title | Santin and cirsimaritin from Betula pubescens and Betula pendula buds induce apoptosis in human digestive system cancer cells |
title_full | Santin and cirsimaritin from Betula pubescens and Betula pendula buds induce apoptosis in human digestive system cancer cells |
title_fullStr | Santin and cirsimaritin from Betula pubescens and Betula pendula buds induce apoptosis in human digestive system cancer cells |
title_full_unstemmed | Santin and cirsimaritin from Betula pubescens and Betula pendula buds induce apoptosis in human digestive system cancer cells |
title_short | Santin and cirsimaritin from Betula pubescens and Betula pendula buds induce apoptosis in human digestive system cancer cells |
title_sort | santin and cirsimaritin from betula pubescens and betula pendula buds induce apoptosis in human digestive system cancer cells |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8650031/ https://www.ncbi.nlm.nih.gov/pubmed/34755444 http://dx.doi.org/10.1111/jcmm.17031 |
work_keys_str_mv | AT szokalukasz santinandcirsimaritinfrombetulapubescensandbetulapendulabudsinduceapoptosisinhumandigestivesystemcancercells AT nazarukjolanta santinandcirsimaritinfrombetulapubescensandbetulapendulabudsinduceapoptosisinhumandigestivesystemcancercells AT stockimarcin santinandcirsimaritinfrombetulapubescensandbetulapendulabudsinduceapoptosisinhumandigestivesystemcancercells AT isidorovvalery santinandcirsimaritinfrombetulapubescensandbetulapendulabudsinduceapoptosisinhumandigestivesystemcancercells |