Cargando…
β2SP/TET2 complex regulates gene 5hmC modification after cerebral ischemia
βII spectrin (β2SP) is encoded by Sptbn1 and is involved in the regulation of various cell functions. β2SP contributes to the formation of the myelin sheath, which may be related to the mechanism of neuropathy caused by demyelination. As one of the main features of cerebral ischemia, demyelination p...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8650033/ https://www.ncbi.nlm.nih.gov/pubmed/34799994 http://dx.doi.org/10.1111/jcmm.17060 |
_version_ | 1784611122596282368 |
---|---|
author | Ma, Xiaohua Zhang, Meng Yan, Rui Wu, Hainan Yang, Bo Miao, Zhigang |
author_facet | Ma, Xiaohua Zhang, Meng Yan, Rui Wu, Hainan Yang, Bo Miao, Zhigang |
author_sort | Ma, Xiaohua |
collection | PubMed |
description | βII spectrin (β2SP) is encoded by Sptbn1 and is involved in the regulation of various cell functions. β2SP contributes to the formation of the myelin sheath, which may be related to the mechanism of neuropathy caused by demyelination. As one of the main features of cerebral ischemia, demyelination plays a key role in the mechanism of cerebral ischemia injury. Here, we showed that β2SP levels were increased, and this molecule interacted with TET2 after ischemic injury. Furthermore, we found that the level of TET2 was decreased in the nucleus when β2SP was knocked out after oxygen and glucose deprivation (OGD), and the level of 5hmC was reduced in the OGD+β2SP KO group. In contrast, the expression of β2SP did not change in TET2 KO mice. In addition, the 5hmC sequencing results revealed that β2SP can affect the level of 5hmC, the differentially hydroxymethylated region (DhMR) mainly related with the Calcium signalling pathway, cGMP‐PKG signalling pathway, Wnt signalling pathway and Hippo signalling pathway. In summary, our results suggest that β2SP could regulate the gene 5hmC by interacted with TET2 and will become a potential therapeutic target for ischemic stroke. |
format | Online Article Text |
id | pubmed-8650033 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86500332021-12-20 β2SP/TET2 complex regulates gene 5hmC modification after cerebral ischemia Ma, Xiaohua Zhang, Meng Yan, Rui Wu, Hainan Yang, Bo Miao, Zhigang J Cell Mol Med Original Articles βII spectrin (β2SP) is encoded by Sptbn1 and is involved in the regulation of various cell functions. β2SP contributes to the formation of the myelin sheath, which may be related to the mechanism of neuropathy caused by demyelination. As one of the main features of cerebral ischemia, demyelination plays a key role in the mechanism of cerebral ischemia injury. Here, we showed that β2SP levels were increased, and this molecule interacted with TET2 after ischemic injury. Furthermore, we found that the level of TET2 was decreased in the nucleus when β2SP was knocked out after oxygen and glucose deprivation (OGD), and the level of 5hmC was reduced in the OGD+β2SP KO group. In contrast, the expression of β2SP did not change in TET2 KO mice. In addition, the 5hmC sequencing results revealed that β2SP can affect the level of 5hmC, the differentially hydroxymethylated region (DhMR) mainly related with the Calcium signalling pathway, cGMP‐PKG signalling pathway, Wnt signalling pathway and Hippo signalling pathway. In summary, our results suggest that β2SP could regulate the gene 5hmC by interacted with TET2 and will become a potential therapeutic target for ischemic stroke. John Wiley and Sons Inc. 2021-11-19 2021-12 /pmc/articles/PMC8650033/ /pubmed/34799994 http://dx.doi.org/10.1111/jcmm.17060 Text en © 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Ma, Xiaohua Zhang, Meng Yan, Rui Wu, Hainan Yang, Bo Miao, Zhigang β2SP/TET2 complex regulates gene 5hmC modification after cerebral ischemia |
title | β2SP/TET2 complex regulates gene 5hmC modification after cerebral ischemia |
title_full | β2SP/TET2 complex regulates gene 5hmC modification after cerebral ischemia |
title_fullStr | β2SP/TET2 complex regulates gene 5hmC modification after cerebral ischemia |
title_full_unstemmed | β2SP/TET2 complex regulates gene 5hmC modification after cerebral ischemia |
title_short | β2SP/TET2 complex regulates gene 5hmC modification after cerebral ischemia |
title_sort | β2sp/tet2 complex regulates gene 5hmc modification after cerebral ischemia |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8650033/ https://www.ncbi.nlm.nih.gov/pubmed/34799994 http://dx.doi.org/10.1111/jcmm.17060 |
work_keys_str_mv | AT maxiaohua b2sptet2complexregulatesgene5hmcmodificationaftercerebralischemia AT zhangmeng b2sptet2complexregulatesgene5hmcmodificationaftercerebralischemia AT yanrui b2sptet2complexregulatesgene5hmcmodificationaftercerebralischemia AT wuhainan b2sptet2complexregulatesgene5hmcmodificationaftercerebralischemia AT yangbo b2sptet2complexregulatesgene5hmcmodificationaftercerebralischemia AT miaozhigang b2sptet2complexregulatesgene5hmcmodificationaftercerebralischemia |