Quantifying the effect of PEG architecture on nanoparticle ligand availability using DNA-PAINT

The importance of PEG architecture on nanoparticle (NP) functionality is known but still difficult to investigate, especially at a single particle level. Here, we apply DNA Point Accumulation for Imaging in Nanoscale Topography (DNA-PAINT), a super-resolution microscopy (SRM) technique, to study the...

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Detalles Bibliográficos
Autores principales: Andrian, Teodora, Pujals, Silvia, Albertazzi, Lorenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8650147/
https://www.ncbi.nlm.nih.gov/pubmed/34977461
http://dx.doi.org/10.1039/d1na00696g
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author Andrian, Teodora
Pujals, Silvia
Albertazzi, Lorenzo
author_facet Andrian, Teodora
Pujals, Silvia
Albertazzi, Lorenzo
author_sort Andrian, Teodora
collection PubMed
description The importance of PEG architecture on nanoparticle (NP) functionality is known but still difficult to investigate, especially at a single particle level. Here, we apply DNA Point Accumulation for Imaging in Nanoscale Topography (DNA-PAINT), a super-resolution microscopy (SRM) technique, to study the surface functionality in poly(lactide-co-glycolide)–poly(ethylene glycol) (PLGA–PEG) NPs with different PEG structures. We demonstrated how the length of the PEG spacer can influence the accessibility of surface chemical functionality, highlighting the importance of SRM techniques to support the rational design of functionalized NPs.
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spelling pubmed-86501472021-12-30 Quantifying the effect of PEG architecture on nanoparticle ligand availability using DNA-PAINT Andrian, Teodora Pujals, Silvia Albertazzi, Lorenzo Nanoscale Adv Chemistry The importance of PEG architecture on nanoparticle (NP) functionality is known but still difficult to investigate, especially at a single particle level. Here, we apply DNA Point Accumulation for Imaging in Nanoscale Topography (DNA-PAINT), a super-resolution microscopy (SRM) technique, to study the surface functionality in poly(lactide-co-glycolide)–poly(ethylene glycol) (PLGA–PEG) NPs with different PEG structures. We demonstrated how the length of the PEG spacer can influence the accessibility of surface chemical functionality, highlighting the importance of SRM techniques to support the rational design of functionalized NPs. RSC 2021-11-01 /pmc/articles/PMC8650147/ /pubmed/34977461 http://dx.doi.org/10.1039/d1na00696g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Andrian, Teodora
Pujals, Silvia
Albertazzi, Lorenzo
Quantifying the effect of PEG architecture on nanoparticle ligand availability using DNA-PAINT
title Quantifying the effect of PEG architecture on nanoparticle ligand availability using DNA-PAINT
title_full Quantifying the effect of PEG architecture on nanoparticle ligand availability using DNA-PAINT
title_fullStr Quantifying the effect of PEG architecture on nanoparticle ligand availability using DNA-PAINT
title_full_unstemmed Quantifying the effect of PEG architecture on nanoparticle ligand availability using DNA-PAINT
title_short Quantifying the effect of PEG architecture on nanoparticle ligand availability using DNA-PAINT
title_sort quantifying the effect of peg architecture on nanoparticle ligand availability using dna-paint
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8650147/
https://www.ncbi.nlm.nih.gov/pubmed/34977461
http://dx.doi.org/10.1039/d1na00696g
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