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Cardiac impact of high-frequency irreversible electroporation using an asymmetrical waveform on liver in vivo

BACKGROUND: High-Frequency Irreversible Electroporation (H-FIRE) is a novel technology for non-thermal ablation. Different from Irreversible electroporation (IRE), H-FIRE delivers bipolar electrical pulses without muscle contraction and does not cause electrolysis. Currently, little is known regardi...

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Autores principales: Li, Jing, Wang, Jingjing, Zhang, Xiaobo, Zhang, Xiao, Gao, Hongmei, Xiao, Yueyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8650563/
https://www.ncbi.nlm.nih.gov/pubmed/34876030
http://dx.doi.org/10.1186/s12872-021-02412-9
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author Li, Jing
Wang, Jingjing
Zhang, Xiaobo
Zhang, Xiao
Gao, Hongmei
Xiao, Yueyong
author_facet Li, Jing
Wang, Jingjing
Zhang, Xiaobo
Zhang, Xiao
Gao, Hongmei
Xiao, Yueyong
author_sort Li, Jing
collection PubMed
description BACKGROUND: High-Frequency Irreversible Electroporation (H-FIRE) is a novel technology for non-thermal ablation. Different from Irreversible electroporation (IRE), H-FIRE delivers bipolar electrical pulses without muscle contraction and does not cause electrolysis. Currently, little is known regarding the cardiac safety during the administration of H-FIRE on liver. The aim of this study was to evaluate the changes of electrocardiogram (ECG) and biomarkers of cardiac damage during asymmetrical waveform of H-FIRE therapy in vivo. METHODS: The swines (n = 7) in IRE group, which used 100 pulses (2200 V, 100–100 μs configuration), were administrated with muscle relaxant under anesthesia. In the absence of muscle relaxant, 7 swines in H-FIRE group were performed with 2400 pulses (3000 V, 5–3–3–5 μs configuration). Midazolam (0.5 mg/kg) and xylazine hydrochloride (20 mg/kg) were given to induce sedation, followed by Isoflurane (2.5%, 100% oxygen, 3 L/min) to maintain sedation in all the swines. Limb lead ECG recordings were analyzed by two electrophysiologists to judge the arrhythmia. Cardiac and liver tissue was examined by pathology technique. RESULTS: The ablation zones were larger in H-FIRE than IRE. Both IRE and H-FIRE did not affect the autonomous cardiac rhythm. Even when the electrical signal of IRE and H-FIRE fell on ventricular vulnerable period. Moreover, cTnI in IRE group showed an increase in 4 h after ablation, and decreased to baseline 72 h after ablation. However, cTnI showed no significant change during the administration of H-FIRE. CONCLUSIONS: The study suggests an asymmetrical waveform for H-FIRE is a promising measure for liver ablation. The results were based on normal liver and the swines without potential cardiac diseases. With the limitations of these facts, asymmetrical waveform for H-FIRE of liver tissue seems relatively safe without major cardiac complications. The safety of asymmetrical waveform for H-FIRE needs to evaluate in future.
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spelling pubmed-86505632021-12-08 Cardiac impact of high-frequency irreversible electroporation using an asymmetrical waveform on liver in vivo Li, Jing Wang, Jingjing Zhang, Xiaobo Zhang, Xiao Gao, Hongmei Xiao, Yueyong BMC Cardiovasc Disord Research BACKGROUND: High-Frequency Irreversible Electroporation (H-FIRE) is a novel technology for non-thermal ablation. Different from Irreversible electroporation (IRE), H-FIRE delivers bipolar electrical pulses without muscle contraction and does not cause electrolysis. Currently, little is known regarding the cardiac safety during the administration of H-FIRE on liver. The aim of this study was to evaluate the changes of electrocardiogram (ECG) and biomarkers of cardiac damage during asymmetrical waveform of H-FIRE therapy in vivo. METHODS: The swines (n = 7) in IRE group, which used 100 pulses (2200 V, 100–100 μs configuration), were administrated with muscle relaxant under anesthesia. In the absence of muscle relaxant, 7 swines in H-FIRE group were performed with 2400 pulses (3000 V, 5–3–3–5 μs configuration). Midazolam (0.5 mg/kg) and xylazine hydrochloride (20 mg/kg) were given to induce sedation, followed by Isoflurane (2.5%, 100% oxygen, 3 L/min) to maintain sedation in all the swines. Limb lead ECG recordings were analyzed by two electrophysiologists to judge the arrhythmia. Cardiac and liver tissue was examined by pathology technique. RESULTS: The ablation zones were larger in H-FIRE than IRE. Both IRE and H-FIRE did not affect the autonomous cardiac rhythm. Even when the electrical signal of IRE and H-FIRE fell on ventricular vulnerable period. Moreover, cTnI in IRE group showed an increase in 4 h after ablation, and decreased to baseline 72 h after ablation. However, cTnI showed no significant change during the administration of H-FIRE. CONCLUSIONS: The study suggests an asymmetrical waveform for H-FIRE is a promising measure for liver ablation. The results were based on normal liver and the swines without potential cardiac diseases. With the limitations of these facts, asymmetrical waveform for H-FIRE of liver tissue seems relatively safe without major cardiac complications. The safety of asymmetrical waveform for H-FIRE needs to evaluate in future. BioMed Central 2021-12-07 /pmc/articles/PMC8650563/ /pubmed/34876030 http://dx.doi.org/10.1186/s12872-021-02412-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Li, Jing
Wang, Jingjing
Zhang, Xiaobo
Zhang, Xiao
Gao, Hongmei
Xiao, Yueyong
Cardiac impact of high-frequency irreversible electroporation using an asymmetrical waveform on liver in vivo
title Cardiac impact of high-frequency irreversible electroporation using an asymmetrical waveform on liver in vivo
title_full Cardiac impact of high-frequency irreversible electroporation using an asymmetrical waveform on liver in vivo
title_fullStr Cardiac impact of high-frequency irreversible electroporation using an asymmetrical waveform on liver in vivo
title_full_unstemmed Cardiac impact of high-frequency irreversible electroporation using an asymmetrical waveform on liver in vivo
title_short Cardiac impact of high-frequency irreversible electroporation using an asymmetrical waveform on liver in vivo
title_sort cardiac impact of high-frequency irreversible electroporation using an asymmetrical waveform on liver in vivo
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8650563/
https://www.ncbi.nlm.nih.gov/pubmed/34876030
http://dx.doi.org/10.1186/s12872-021-02412-9
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