Cargando…
Tapetal 3-Ketoacyl-Coenzyme A Synthases Are Involved in Pollen Coat Lipid Accumulation for Pollen-Stigma Interaction in Arabidopsis
Pollen coat lipids form an outer barrier to protect pollen itself and play essential roles in pollen-stigma interaction. However, the precise molecular mechanisms underlying the production, deposition, regulation, and function of pollen coat lipids during anther development remain largely elusive. I...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8650583/ https://www.ncbi.nlm.nih.gov/pubmed/34887893 http://dx.doi.org/10.3389/fpls.2021.770311 |
_version_ | 1784611228549644288 |
---|---|
author | Zhang, Zaibao Zhan, Huadong Lu, Jieyang Xiong, Shuangxi Yang, Naiying Yuan, Hongyu Yang, Zhong-Nan |
author_facet | Zhang, Zaibao Zhan, Huadong Lu, Jieyang Xiong, Shuangxi Yang, Naiying Yuan, Hongyu Yang, Zhong-Nan |
author_sort | Zhang, Zaibao |
collection | PubMed |
description | Pollen coat lipids form an outer barrier to protect pollen itself and play essential roles in pollen-stigma interaction. However, the precise molecular mechanisms underlying the production, deposition, regulation, and function of pollen coat lipids during anther development remain largely elusive. In lipid metabolism, 3-ketoacyl-coenzyme A synthases (KCS) are involved in fatty acid elongation or very-long-chain fatty acid (VLCFA) synthesis. In this study, we identified six members of the Arabidopsis KCS family expressed in anther. Among them, KCS7, KCS15, and KCS21 were expressed in tapetal cells at anther stages 8–10. Further analysis demonstrated that they act downstream of male sterility 1 (MS1), a regulator of late tapetum development. The kcs7/15/21 triple mutant is fertile. Both cellular observation and lipid staining showed pollen coat lipid was decreased in kcs7/15/21 triple mutant. After landing on stigma, the wild-type pollen grains were hydrated for about 5 min while the kcs7/15/21 triple mutant pollen took about 10 min to hydrate. Pollen tube growth of the triple mutant was also delayed. These results demonstrate that the tapetum-localized KCS proteins are involved in the accumulation of pollen coat lipid and reveal the roles of tapetal-derived pollen coat lipid for pollen-stigma interaction. |
format | Online Article Text |
id | pubmed-8650583 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86505832021-12-08 Tapetal 3-Ketoacyl-Coenzyme A Synthases Are Involved in Pollen Coat Lipid Accumulation for Pollen-Stigma Interaction in Arabidopsis Zhang, Zaibao Zhan, Huadong Lu, Jieyang Xiong, Shuangxi Yang, Naiying Yuan, Hongyu Yang, Zhong-Nan Front Plant Sci Plant Science Pollen coat lipids form an outer barrier to protect pollen itself and play essential roles in pollen-stigma interaction. However, the precise molecular mechanisms underlying the production, deposition, regulation, and function of pollen coat lipids during anther development remain largely elusive. In lipid metabolism, 3-ketoacyl-coenzyme A synthases (KCS) are involved in fatty acid elongation or very-long-chain fatty acid (VLCFA) synthesis. In this study, we identified six members of the Arabidopsis KCS family expressed in anther. Among them, KCS7, KCS15, and KCS21 were expressed in tapetal cells at anther stages 8–10. Further analysis demonstrated that they act downstream of male sterility 1 (MS1), a regulator of late tapetum development. The kcs7/15/21 triple mutant is fertile. Both cellular observation and lipid staining showed pollen coat lipid was decreased in kcs7/15/21 triple mutant. After landing on stigma, the wild-type pollen grains were hydrated for about 5 min while the kcs7/15/21 triple mutant pollen took about 10 min to hydrate. Pollen tube growth of the triple mutant was also delayed. These results demonstrate that the tapetum-localized KCS proteins are involved in the accumulation of pollen coat lipid and reveal the roles of tapetal-derived pollen coat lipid for pollen-stigma interaction. Frontiers Media S.A. 2021-11-23 /pmc/articles/PMC8650583/ /pubmed/34887893 http://dx.doi.org/10.3389/fpls.2021.770311 Text en Copyright © 2021 Zhang, Zhan, Lu, Xiong, Yang, Yuan and Yang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Zhang, Zaibao Zhan, Huadong Lu, Jieyang Xiong, Shuangxi Yang, Naiying Yuan, Hongyu Yang, Zhong-Nan Tapetal 3-Ketoacyl-Coenzyme A Synthases Are Involved in Pollen Coat Lipid Accumulation for Pollen-Stigma Interaction in Arabidopsis |
title | Tapetal 3-Ketoacyl-Coenzyme A Synthases Are Involved in Pollen Coat Lipid Accumulation for Pollen-Stigma Interaction in Arabidopsis |
title_full | Tapetal 3-Ketoacyl-Coenzyme A Synthases Are Involved in Pollen Coat Lipid Accumulation for Pollen-Stigma Interaction in Arabidopsis |
title_fullStr | Tapetal 3-Ketoacyl-Coenzyme A Synthases Are Involved in Pollen Coat Lipid Accumulation for Pollen-Stigma Interaction in Arabidopsis |
title_full_unstemmed | Tapetal 3-Ketoacyl-Coenzyme A Synthases Are Involved in Pollen Coat Lipid Accumulation for Pollen-Stigma Interaction in Arabidopsis |
title_short | Tapetal 3-Ketoacyl-Coenzyme A Synthases Are Involved in Pollen Coat Lipid Accumulation for Pollen-Stigma Interaction in Arabidopsis |
title_sort | tapetal 3-ketoacyl-coenzyme a synthases are involved in pollen coat lipid accumulation for pollen-stigma interaction in arabidopsis |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8650583/ https://www.ncbi.nlm.nih.gov/pubmed/34887893 http://dx.doi.org/10.3389/fpls.2021.770311 |
work_keys_str_mv | AT zhangzaibao tapetal3ketoacylcoenzymeasynthasesareinvolvedinpollencoatlipidaccumulationforpollenstigmainteractioninarabidopsis AT zhanhuadong tapetal3ketoacylcoenzymeasynthasesareinvolvedinpollencoatlipidaccumulationforpollenstigmainteractioninarabidopsis AT lujieyang tapetal3ketoacylcoenzymeasynthasesareinvolvedinpollencoatlipidaccumulationforpollenstigmainteractioninarabidopsis AT xiongshuangxi tapetal3ketoacylcoenzymeasynthasesareinvolvedinpollencoatlipidaccumulationforpollenstigmainteractioninarabidopsis AT yangnaiying tapetal3ketoacylcoenzymeasynthasesareinvolvedinpollencoatlipidaccumulationforpollenstigmainteractioninarabidopsis AT yuanhongyu tapetal3ketoacylcoenzymeasynthasesareinvolvedinpollencoatlipidaccumulationforpollenstigmainteractioninarabidopsis AT yangzhongnan tapetal3ketoacylcoenzymeasynthasesareinvolvedinpollencoatlipidaccumulationforpollenstigmainteractioninarabidopsis |