Performance comparison of micro-neutralization assays based on surrogate SARS-CoV-2 and WT SARS-CoV-2 in assessing virus-neutralizing capacity of anti-SARS-CoV-2 antibodies

We compared neutralization assays using either the wild-type severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus or surrogate neutralization markers, using characterized sera. We found the results of the neutralization assays 75 % concordant overall and 80 % concordant for samples wit...

Descripción completa

Detalles Bibliográficos
Autores principales: Sekirov, Inna, Petric, Martin, Carruthers, Erin, Lawrence, David, Pidduck, Tamara, Kustra, Jesse, Laley, Jonathan, Lee, Min-Kuang, Chahil, Navdeep, Mak, Annie, Levett, Paul N., Mendoza, Emelissa, Wood, Heidi, Drebot, Mike, Krajden, Mel, Morshed, Muhammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2021
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8650845/
https://www.ncbi.nlm.nih.gov/pubmed/34888485
http://dx.doi.org/10.1099/acmi.0.000257
_version_ 1784611283729907712
author Sekirov, Inna
Petric, Martin
Carruthers, Erin
Lawrence, David
Pidduck, Tamara
Kustra, Jesse
Laley, Jonathan
Lee, Min-Kuang
Chahil, Navdeep
Mak, Annie
Levett, Paul N.
Mendoza, Emelissa
Wood, Heidi
Drebot, Mike
Krajden, Mel
Morshed, Muhammad
author_facet Sekirov, Inna
Petric, Martin
Carruthers, Erin
Lawrence, David
Pidduck, Tamara
Kustra, Jesse
Laley, Jonathan
Lee, Min-Kuang
Chahil, Navdeep
Mak, Annie
Levett, Paul N.
Mendoza, Emelissa
Wood, Heidi
Drebot, Mike
Krajden, Mel
Morshed, Muhammad
author_sort Sekirov, Inna
collection PubMed
description We compared neutralization assays using either the wild-type severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus or surrogate neutralization markers, using characterized sera. We found the results of the neutralization assays 75 % concordant overall and 80 % concordant for samples with high antibody levels. This demonstrates that commercial surrogate SARS-CoV-2 assays offer the potential to assess anti-SARS-CoV-2 antibodies’ neutralizing capacity outside CL-3 laboratory containment.
format Online
Article
Text
id pubmed-8650845
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Microbiology Society
record_format MEDLINE/PubMed
spelling pubmed-86508452021-12-08 Performance comparison of micro-neutralization assays based on surrogate SARS-CoV-2 and WT SARS-CoV-2 in assessing virus-neutralizing capacity of anti-SARS-CoV-2 antibodies Sekirov, Inna Petric, Martin Carruthers, Erin Lawrence, David Pidduck, Tamara Kustra, Jesse Laley, Jonathan Lee, Min-Kuang Chahil, Navdeep Mak, Annie Levett, Paul N. Mendoza, Emelissa Wood, Heidi Drebot, Mike Krajden, Mel Morshed, Muhammad Access Microbiol Short Communications We compared neutralization assays using either the wild-type severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus or surrogate neutralization markers, using characterized sera. We found the results of the neutralization assays 75 % concordant overall and 80 % concordant for samples with high antibody levels. This demonstrates that commercial surrogate SARS-CoV-2 assays offer the potential to assess anti-SARS-CoV-2 antibodies’ neutralizing capacity outside CL-3 laboratory containment. Microbiology Society 2021-08-26 /pmc/articles/PMC8650845/ /pubmed/34888485 http://dx.doi.org/10.1099/acmi.0.000257 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
spellingShingle Short Communications
Sekirov, Inna
Petric, Martin
Carruthers, Erin
Lawrence, David
Pidduck, Tamara
Kustra, Jesse
Laley, Jonathan
Lee, Min-Kuang
Chahil, Navdeep
Mak, Annie
Levett, Paul N.
Mendoza, Emelissa
Wood, Heidi
Drebot, Mike
Krajden, Mel
Morshed, Muhammad
Performance comparison of micro-neutralization assays based on surrogate SARS-CoV-2 and WT SARS-CoV-2 in assessing virus-neutralizing capacity of anti-SARS-CoV-2 antibodies
title Performance comparison of micro-neutralization assays based on surrogate SARS-CoV-2 and WT SARS-CoV-2 in assessing virus-neutralizing capacity of anti-SARS-CoV-2 antibodies
title_full Performance comparison of micro-neutralization assays based on surrogate SARS-CoV-2 and WT SARS-CoV-2 in assessing virus-neutralizing capacity of anti-SARS-CoV-2 antibodies
title_fullStr Performance comparison of micro-neutralization assays based on surrogate SARS-CoV-2 and WT SARS-CoV-2 in assessing virus-neutralizing capacity of anti-SARS-CoV-2 antibodies
title_full_unstemmed Performance comparison of micro-neutralization assays based on surrogate SARS-CoV-2 and WT SARS-CoV-2 in assessing virus-neutralizing capacity of anti-SARS-CoV-2 antibodies
title_short Performance comparison of micro-neutralization assays based on surrogate SARS-CoV-2 and WT SARS-CoV-2 in assessing virus-neutralizing capacity of anti-SARS-CoV-2 antibodies
title_sort performance comparison of micro-neutralization assays based on surrogate sars-cov-2 and wt sars-cov-2 in assessing virus-neutralizing capacity of anti-sars-cov-2 antibodies
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8650845/
https://www.ncbi.nlm.nih.gov/pubmed/34888485
http://dx.doi.org/10.1099/acmi.0.000257
work_keys_str_mv AT sekirovinna performancecomparisonofmicroneutralizationassaysbasedonsurrogatesarscov2andwtsarscov2inassessingvirusneutralizingcapacityofantisarscov2antibodies
AT petricmartin performancecomparisonofmicroneutralizationassaysbasedonsurrogatesarscov2andwtsarscov2inassessingvirusneutralizingcapacityofantisarscov2antibodies
AT carrutherserin performancecomparisonofmicroneutralizationassaysbasedonsurrogatesarscov2andwtsarscov2inassessingvirusneutralizingcapacityofantisarscov2antibodies
AT lawrencedavid performancecomparisonofmicroneutralizationassaysbasedonsurrogatesarscov2andwtsarscov2inassessingvirusneutralizingcapacityofantisarscov2antibodies
AT pidducktamara performancecomparisonofmicroneutralizationassaysbasedonsurrogatesarscov2andwtsarscov2inassessingvirusneutralizingcapacityofantisarscov2antibodies
AT kustrajesse performancecomparisonofmicroneutralizationassaysbasedonsurrogatesarscov2andwtsarscov2inassessingvirusneutralizingcapacityofantisarscov2antibodies
AT laleyjonathan performancecomparisonofmicroneutralizationassaysbasedonsurrogatesarscov2andwtsarscov2inassessingvirusneutralizingcapacityofantisarscov2antibodies
AT leeminkuang performancecomparisonofmicroneutralizationassaysbasedonsurrogatesarscov2andwtsarscov2inassessingvirusneutralizingcapacityofantisarscov2antibodies
AT chahilnavdeep performancecomparisonofmicroneutralizationassaysbasedonsurrogatesarscov2andwtsarscov2inassessingvirusneutralizingcapacityofantisarscov2antibodies
AT makannie performancecomparisonofmicroneutralizationassaysbasedonsurrogatesarscov2andwtsarscov2inassessingvirusneutralizingcapacityofantisarscov2antibodies
AT levettpauln performancecomparisonofmicroneutralizationassaysbasedonsurrogatesarscov2andwtsarscov2inassessingvirusneutralizingcapacityofantisarscov2antibodies
AT mendozaemelissa performancecomparisonofmicroneutralizationassaysbasedonsurrogatesarscov2andwtsarscov2inassessingvirusneutralizingcapacityofantisarscov2antibodies
AT woodheidi performancecomparisonofmicroneutralizationassaysbasedonsurrogatesarscov2andwtsarscov2inassessingvirusneutralizingcapacityofantisarscov2antibodies
AT drebotmike performancecomparisonofmicroneutralizationassaysbasedonsurrogatesarscov2andwtsarscov2inassessingvirusneutralizingcapacityofantisarscov2antibodies
AT krajdenmel performancecomparisonofmicroneutralizationassaysbasedonsurrogatesarscov2andwtsarscov2inassessingvirusneutralizingcapacityofantisarscov2antibodies
AT morshedmuhammad performancecomparisonofmicroneutralizationassaysbasedonsurrogatesarscov2andwtsarscov2inassessingvirusneutralizingcapacityofantisarscov2antibodies

Ejemplares similares