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Diversity and distribution of the tick-borne relapsing fever spirochete Borrelia turicatae

Borrelia turicatae is a causative agent of tick-borne relapsing fever (TBRF) in the subtropics and tropics of the United States and Latin America. Historically, B. turicatae was thought to be maintained in enzootic cycles in rural areas. However, there is growing evidence that suggests the pathogen...

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Autores principales: Krishnavajhala, Aparna, Armstrong, Brittany A., Kneubehl, Alexander R., Gunter, Sarah M., Piccione, Julie, Kim, Hee J., Ramirez, Rosa, Castro-Arellano, Ivan, Roachell, Walter, Teel, Pete D., Lopez, Job E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8651100/
https://www.ncbi.nlm.nih.gov/pubmed/34813588
http://dx.doi.org/10.1371/journal.pntd.0009868
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author Krishnavajhala, Aparna
Armstrong, Brittany A.
Kneubehl, Alexander R.
Gunter, Sarah M.
Piccione, Julie
Kim, Hee J.
Ramirez, Rosa
Castro-Arellano, Ivan
Roachell, Walter
Teel, Pete D.
Lopez, Job E.
author_facet Krishnavajhala, Aparna
Armstrong, Brittany A.
Kneubehl, Alexander R.
Gunter, Sarah M.
Piccione, Julie
Kim, Hee J.
Ramirez, Rosa
Castro-Arellano, Ivan
Roachell, Walter
Teel, Pete D.
Lopez, Job E.
author_sort Krishnavajhala, Aparna
collection PubMed
description Borrelia turicatae is a causative agent of tick-borne relapsing fever (TBRF) in the subtropics and tropics of the United States and Latin America. Historically, B. turicatae was thought to be maintained in enzootic cycles in rural areas. However, there is growing evidence that suggests the pathogen has established endemic foci in densely populated regions of Texas. With the growth of homelessness in the state and human activity in city parks, it was important to implement field collection efforts to identify areas where B. turicatae and its vector circulate. Between 2017 and 2020 we collected Ornithodoros turicata ticks in suburban and urban areas including public and private parks and recreational spaces. Ticks were fed on naïve mice and spirochetes were isolated from the blood. Multilocus sequence typing (MLST) was performed on eight newly obtained isolates and included previously reported sequences. The four chromosomal loci targeted for MLST were 16S ribosomal RNA (rrs), flagellin B (flaB), DNA gyrase B (gyrB), and the intergenic spacer (IGS). Given the complexity of Borrelia genomes, plasmid diversity was also evaluated. These studies indicate that the IGS locus segregates B. turicatae into four genomic types and plasmid diversity is extensive between isolates. Furthermore, B. turicatae and its vector have established endemic foci in parks and recreational areas in densely populated settings of Texas.
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spelling pubmed-86511002021-12-08 Diversity and distribution of the tick-borne relapsing fever spirochete Borrelia turicatae Krishnavajhala, Aparna Armstrong, Brittany A. Kneubehl, Alexander R. Gunter, Sarah M. Piccione, Julie Kim, Hee J. Ramirez, Rosa Castro-Arellano, Ivan Roachell, Walter Teel, Pete D. Lopez, Job E. PLoS Negl Trop Dis Research Article Borrelia turicatae is a causative agent of tick-borne relapsing fever (TBRF) in the subtropics and tropics of the United States and Latin America. Historically, B. turicatae was thought to be maintained in enzootic cycles in rural areas. However, there is growing evidence that suggests the pathogen has established endemic foci in densely populated regions of Texas. With the growth of homelessness in the state and human activity in city parks, it was important to implement field collection efforts to identify areas where B. turicatae and its vector circulate. Between 2017 and 2020 we collected Ornithodoros turicata ticks in suburban and urban areas including public and private parks and recreational spaces. Ticks were fed on naïve mice and spirochetes were isolated from the blood. Multilocus sequence typing (MLST) was performed on eight newly obtained isolates and included previously reported sequences. The four chromosomal loci targeted for MLST were 16S ribosomal RNA (rrs), flagellin B (flaB), DNA gyrase B (gyrB), and the intergenic spacer (IGS). Given the complexity of Borrelia genomes, plasmid diversity was also evaluated. These studies indicate that the IGS locus segregates B. turicatae into four genomic types and plasmid diversity is extensive between isolates. Furthermore, B. turicatae and its vector have established endemic foci in parks and recreational areas in densely populated settings of Texas. Public Library of Science 2021-11-23 /pmc/articles/PMC8651100/ /pubmed/34813588 http://dx.doi.org/10.1371/journal.pntd.0009868 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Krishnavajhala, Aparna
Armstrong, Brittany A.
Kneubehl, Alexander R.
Gunter, Sarah M.
Piccione, Julie
Kim, Hee J.
Ramirez, Rosa
Castro-Arellano, Ivan
Roachell, Walter
Teel, Pete D.
Lopez, Job E.
Diversity and distribution of the tick-borne relapsing fever spirochete Borrelia turicatae
title Diversity and distribution of the tick-borne relapsing fever spirochete Borrelia turicatae
title_full Diversity and distribution of the tick-borne relapsing fever spirochete Borrelia turicatae
title_fullStr Diversity and distribution of the tick-borne relapsing fever spirochete Borrelia turicatae
title_full_unstemmed Diversity and distribution of the tick-borne relapsing fever spirochete Borrelia turicatae
title_short Diversity and distribution of the tick-borne relapsing fever spirochete Borrelia turicatae
title_sort diversity and distribution of the tick-borne relapsing fever spirochete borrelia turicatae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8651100/
https://www.ncbi.nlm.nih.gov/pubmed/34813588
http://dx.doi.org/10.1371/journal.pntd.0009868
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