Cargando…
The use of HRM shifts in qPCR to investigate a much neglected aspect of interference by intracellular nanoparticles
Genetic molecular studies used to understand potential risks of engineered nanomaterials (ENMs) are incomplete. Intracellular residual ENMs present in biological samples may cause assay interference. This report applies the high-resolution melt (HRM) feature of RT-qPCR to detect shifts caused by the...
Autores principales: | Sanabria, Natasha M., Gulumian, Mary |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8651142/ https://www.ncbi.nlm.nih.gov/pubmed/34874941 http://dx.doi.org/10.1371/journal.pone.0260207 |
Ejemplares similares
-
The presence of residual gold nanoparticles in samples interferes with the RT-qPCR assay used for gene expression profiling
por: Sanabria, Natasha M., et al.
Publicado: (2017) -
Detection and quantification of Spirocerca lupi by HRM qPCR in fecal samples from dogs with spirocercosis
por: Rojas, Alicia, et al.
Publicado: (2017) -
A novel 5-Plex qPCR-HRM assay detecting human diarrheal parasites
por: Lamien-Meda, Aline, et al.
Publicado: (2020) -
Detection of SARS-CoV-2 spike protein D614G mutation by qPCR-HRM analysis
por: Gazali, Faris Muhammad, et al.
Publicado: (2021) -
Two Leishmania species separation targeting the ITS-rDNA and Cyt b genes by developing and evaluating HRM- qPCR
por: Alaeenovin, Elnaz, et al.
Publicado: (2022)