Cargando…
Nematicidal Activity of Cyclopiazonic Acid Derived From Penicillium commune Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process
Among 200 fungal strains isolated from the soil, only one culture filtrate of Aspergillus flavus JCK-4087 showed strong nematicidal activity against Meloidogyne incognita. The nematicidal metabolite isolated from the culture filtrate of JCK-4087 was identified as cyclopiazonic acid (CPA). Because JC...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8651706/ https://www.ncbi.nlm.nih.gov/pubmed/34899622 http://dx.doi.org/10.3389/fmicb.2021.726504 |
_version_ | 1784611456565641216 |
---|---|
author | Nguyen, Van Thi Yu, Nan Hee Lee, Yookyung Hwang, In Min Bui, Hung Xuan Kim, Jin-Cheol |
author_facet | Nguyen, Van Thi Yu, Nan Hee Lee, Yookyung Hwang, In Min Bui, Hung Xuan Kim, Jin-Cheol |
author_sort | Nguyen, Van Thi |
collection | PubMed |
description | Among 200 fungal strains isolated from the soil, only one culture filtrate of Aspergillus flavus JCK-4087 showed strong nematicidal activity against Meloidogyne incognita. The nematicidal metabolite isolated from the culture filtrate of JCK-4087 was identified as cyclopiazonic acid (CPA). Because JCK-4087 also produced aflatoxins, six strains of Penicillium commune, which have been reported to be CPA producers, were obtained from the bank and then tested for their CPA productivity. CPA was isolated from the culture filtrate of P. commune KACC 45973. CPA killed the second-stage juveniles of M. incognita, M. hapla, and M. arearia with EC(50–3 days) 4.50, 18.82, and 60.51 μg mL(–1), respectively. CPA also significantly inhibited egg hatch of M. incognita and M. hapla after a total of 28 days of treatment with the concentrations > 25 μg mL(–1). The enhancement of CPA production by P. commune KACC 45973 was explored using an optimized medium based on Plackett–Burman design (PBD) and central composite design (CCD). The highest CPA production (381.48 μg mL(–1)) was obtained from the optimized medium, exhibiting an increase of 7.88 times when compared with that from potato dextrose broth culture. Application of the wettable power-type formulation of the ethyl acetate extract of the culture filtrate of KACC 45973 reduced gall formation and nematode populations in tomato roots and soils under greenhouse conditions. These results suggest that CPA produced by P. commune KACC 45973 can be used as either a biochemical nematicide or a lead molecule for developing chemical nematicides to control root-knot nematodes. |
format | Online Article Text |
id | pubmed-8651706 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86517062021-12-09 Nematicidal Activity of Cyclopiazonic Acid Derived From Penicillium commune Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process Nguyen, Van Thi Yu, Nan Hee Lee, Yookyung Hwang, In Min Bui, Hung Xuan Kim, Jin-Cheol Front Microbiol Microbiology Among 200 fungal strains isolated from the soil, only one culture filtrate of Aspergillus flavus JCK-4087 showed strong nematicidal activity against Meloidogyne incognita. The nematicidal metabolite isolated from the culture filtrate of JCK-4087 was identified as cyclopiazonic acid (CPA). Because JCK-4087 also produced aflatoxins, six strains of Penicillium commune, which have been reported to be CPA producers, were obtained from the bank and then tested for their CPA productivity. CPA was isolated from the culture filtrate of P. commune KACC 45973. CPA killed the second-stage juveniles of M. incognita, M. hapla, and M. arearia with EC(50–3 days) 4.50, 18.82, and 60.51 μg mL(–1), respectively. CPA also significantly inhibited egg hatch of M. incognita and M. hapla after a total of 28 days of treatment with the concentrations > 25 μg mL(–1). The enhancement of CPA production by P. commune KACC 45973 was explored using an optimized medium based on Plackett–Burman design (PBD) and central composite design (CCD). The highest CPA production (381.48 μg mL(–1)) was obtained from the optimized medium, exhibiting an increase of 7.88 times when compared with that from potato dextrose broth culture. Application of the wettable power-type formulation of the ethyl acetate extract of the culture filtrate of KACC 45973 reduced gall formation and nematode populations in tomato roots and soils under greenhouse conditions. These results suggest that CPA produced by P. commune KACC 45973 can be used as either a biochemical nematicide or a lead molecule for developing chemical nematicides to control root-knot nematodes. Frontiers Media S.A. 2021-11-24 /pmc/articles/PMC8651706/ /pubmed/34899622 http://dx.doi.org/10.3389/fmicb.2021.726504 Text en Copyright © 2021 Nguyen, Yu, Lee, Hwang, Bui and Kim. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Nguyen, Van Thi Yu, Nan Hee Lee, Yookyung Hwang, In Min Bui, Hung Xuan Kim, Jin-Cheol Nematicidal Activity of Cyclopiazonic Acid Derived From Penicillium commune Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process |
title | Nematicidal Activity of Cyclopiazonic Acid Derived From Penicillium commune Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process |
title_full | Nematicidal Activity of Cyclopiazonic Acid Derived From Penicillium commune Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process |
title_fullStr | Nematicidal Activity of Cyclopiazonic Acid Derived From Penicillium commune Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process |
title_full_unstemmed | Nematicidal Activity of Cyclopiazonic Acid Derived From Penicillium commune Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process |
title_short | Nematicidal Activity of Cyclopiazonic Acid Derived From Penicillium commune Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process |
title_sort | nematicidal activity of cyclopiazonic acid derived from penicillium commune against root-knot nematodes and optimization of the culture fermentation process |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8651706/ https://www.ncbi.nlm.nih.gov/pubmed/34899622 http://dx.doi.org/10.3389/fmicb.2021.726504 |
work_keys_str_mv | AT nguyenvanthi nematicidalactivityofcyclopiazonicacidderivedfrompenicilliumcommuneagainstrootknotnematodesandoptimizationoftheculturefermentationprocess AT yunanhee nematicidalactivityofcyclopiazonicacidderivedfrompenicilliumcommuneagainstrootknotnematodesandoptimizationoftheculturefermentationprocess AT leeyookyung nematicidalactivityofcyclopiazonicacidderivedfrompenicilliumcommuneagainstrootknotnematodesandoptimizationoftheculturefermentationprocess AT hwanginmin nematicidalactivityofcyclopiazonicacidderivedfrompenicilliumcommuneagainstrootknotnematodesandoptimizationoftheculturefermentationprocess AT buihungxuan nematicidalactivityofcyclopiazonicacidderivedfrompenicilliumcommuneagainstrootknotnematodesandoptimizationoftheculturefermentationprocess AT kimjincheol nematicidalactivityofcyclopiazonicacidderivedfrompenicilliumcommuneagainstrootknotnematodesandoptimizationoftheculturefermentationprocess |