Cargando…

Hematological and Hematopoietic Effects of Bactericidal Doses of Trans-Cinnamaldehyde and Thyme Oil on Cyprinus carpio Juveniles

The effects of two potential antibacterial agents of plant origin: trans-cinnamaldehyde (TC) and thyme oil (TO) on the peripheral blood parameters and cellular composition of hematopoietic tissue of Cyprinus carpio were studied. Both phytochemicals were used in the doses based on the bactericidal co...

Descripción completa

Detalles Bibliográficos
Autores principales: Kondera, Elżbieta, Bojarski, Bartosz, Ługowska, Katarzyna, Kot, Barbara, Witeska, Małgorzata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8652124/
https://www.ncbi.nlm.nih.gov/pubmed/34899393
http://dx.doi.org/10.3389/fphys.2021.771243
_version_ 1784611525803114496
author Kondera, Elżbieta
Bojarski, Bartosz
Ługowska, Katarzyna
Kot, Barbara
Witeska, Małgorzata
author_facet Kondera, Elżbieta
Bojarski, Bartosz
Ługowska, Katarzyna
Kot, Barbara
Witeska, Małgorzata
author_sort Kondera, Elżbieta
collection PubMed
description The effects of two potential antibacterial agents of plant origin: trans-cinnamaldehyde (TC) and thyme oil (TO) on the peripheral blood parameters and cellular composition of hematopoietic tissue of Cyprinus carpio were studied. Both phytochemicals were used in the doses based on the bactericidal concentrations against Aeromonas spp. developed earlier in in vitro study. The fish were fed for 2 weeks on a commercial feed supplemented with 10 μl/kg of TC or 20 μl/kg of TO. Groups TC1 and TO1 were fed diets containing phytochemicals daily, while groups TC2 and TO2 every 2 days. Control group and groups TC2 and TO2 on the remaining days were fed plain feed. Peripheral blood and head kidney hematopoietic tissue were sampled from all the fish at the end of the experiment. In all the groups, hematological values were within the reference ranges for the healthy common carp juveniles. However, blood hemoglobin (Hb) concentration and mean corpuscular hemoglobin concentration (MCHC) were significantly lower in all the groups exposed to TC and TO, while MCH in TC1, TO1, and TO2 compared to the control. TC and TO did not affect leukocyte count [white blood cell (WBC)], differential leukocyte count, the oxidative activity of phagocytes [nitroblue tetrazolium (NBT)], or thrombocyte count (Thro). No significant alterations were observed in the hematopoietic tissue. The results showed that TC and TO exhibited no considerable hematotoxic effects and trials of their use in the treatment of fish infected with Aeromonas spp. may be undertaken.
format Online
Article
Text
id pubmed-8652124
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-86521242021-12-09 Hematological and Hematopoietic Effects of Bactericidal Doses of Trans-Cinnamaldehyde and Thyme Oil on Cyprinus carpio Juveniles Kondera, Elżbieta Bojarski, Bartosz Ługowska, Katarzyna Kot, Barbara Witeska, Małgorzata Front Physiol Physiology The effects of two potential antibacterial agents of plant origin: trans-cinnamaldehyde (TC) and thyme oil (TO) on the peripheral blood parameters and cellular composition of hematopoietic tissue of Cyprinus carpio were studied. Both phytochemicals were used in the doses based on the bactericidal concentrations against Aeromonas spp. developed earlier in in vitro study. The fish were fed for 2 weeks on a commercial feed supplemented with 10 μl/kg of TC or 20 μl/kg of TO. Groups TC1 and TO1 were fed diets containing phytochemicals daily, while groups TC2 and TO2 every 2 days. Control group and groups TC2 and TO2 on the remaining days were fed plain feed. Peripheral blood and head kidney hematopoietic tissue were sampled from all the fish at the end of the experiment. In all the groups, hematological values were within the reference ranges for the healthy common carp juveniles. However, blood hemoglobin (Hb) concentration and mean corpuscular hemoglobin concentration (MCHC) were significantly lower in all the groups exposed to TC and TO, while MCH in TC1, TO1, and TO2 compared to the control. TC and TO did not affect leukocyte count [white blood cell (WBC)], differential leukocyte count, the oxidative activity of phagocytes [nitroblue tetrazolium (NBT)], or thrombocyte count (Thro). No significant alterations were observed in the hematopoietic tissue. The results showed that TC and TO exhibited no considerable hematotoxic effects and trials of their use in the treatment of fish infected with Aeromonas spp. may be undertaken. Frontiers Media S.A. 2021-11-24 /pmc/articles/PMC8652124/ /pubmed/34899393 http://dx.doi.org/10.3389/fphys.2021.771243 Text en Copyright © 2021 Kondera, Bojarski, Ługowska, Kot and Witeska. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Kondera, Elżbieta
Bojarski, Bartosz
Ługowska, Katarzyna
Kot, Barbara
Witeska, Małgorzata
Hematological and Hematopoietic Effects of Bactericidal Doses of Trans-Cinnamaldehyde and Thyme Oil on Cyprinus carpio Juveniles
title Hematological and Hematopoietic Effects of Bactericidal Doses of Trans-Cinnamaldehyde and Thyme Oil on Cyprinus carpio Juveniles
title_full Hematological and Hematopoietic Effects of Bactericidal Doses of Trans-Cinnamaldehyde and Thyme Oil on Cyprinus carpio Juveniles
title_fullStr Hematological and Hematopoietic Effects of Bactericidal Doses of Trans-Cinnamaldehyde and Thyme Oil on Cyprinus carpio Juveniles
title_full_unstemmed Hematological and Hematopoietic Effects of Bactericidal Doses of Trans-Cinnamaldehyde and Thyme Oil on Cyprinus carpio Juveniles
title_short Hematological and Hematopoietic Effects of Bactericidal Doses of Trans-Cinnamaldehyde and Thyme Oil on Cyprinus carpio Juveniles
title_sort hematological and hematopoietic effects of bactericidal doses of trans-cinnamaldehyde and thyme oil on cyprinus carpio juveniles
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8652124/
https://www.ncbi.nlm.nih.gov/pubmed/34899393
http://dx.doi.org/10.3389/fphys.2021.771243
work_keys_str_mv AT konderaelzbieta hematologicalandhematopoieticeffectsofbactericidaldosesoftranscinnamaldehydeandthymeoiloncyprinuscarpiojuveniles
AT bojarskibartosz hematologicalandhematopoieticeffectsofbactericidaldosesoftranscinnamaldehydeandthymeoiloncyprinuscarpiojuveniles
AT ługowskakatarzyna hematologicalandhematopoieticeffectsofbactericidaldosesoftranscinnamaldehydeandthymeoiloncyprinuscarpiojuveniles
AT kotbarbara hematologicalandhematopoieticeffectsofbactericidaldosesoftranscinnamaldehydeandthymeoiloncyprinuscarpiojuveniles
AT witeskamałgorzata hematologicalandhematopoieticeffectsofbactericidaldosesoftranscinnamaldehydeandthymeoiloncyprinuscarpiojuveniles