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CRISPR/Cas System and Factors Affecting Its Precision and Efficiency

The diverse applications of genetically modified cells and organisms require more precise and efficient genome-editing tool such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas). The CRISPR/Cas system was originally discovered in bacteria as a part...

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Autores principales: Javaid, Nasir, Choi, Sangdun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8652214/
https://www.ncbi.nlm.nih.gov/pubmed/34901007
http://dx.doi.org/10.3389/fcell.2021.761709
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author Javaid, Nasir
Choi, Sangdun
author_facet Javaid, Nasir
Choi, Sangdun
author_sort Javaid, Nasir
collection PubMed
description The diverse applications of genetically modified cells and organisms require more precise and efficient genome-editing tool such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas). The CRISPR/Cas system was originally discovered in bacteria as a part of adaptive-immune system with multiple types. Its engineered versions involve multiple host DNA-repair pathways in order to perform genome editing in host cells. However, it is still challenging to get maximum genome-editing efficiency with fewer or no off-targets. Here, we focused on factors affecting the genome-editing efficiency and precision of CRISPR/Cas system along with its defense-mechanism, orthologues, and applications.
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spelling pubmed-86522142021-12-09 CRISPR/Cas System and Factors Affecting Its Precision and Efficiency Javaid, Nasir Choi, Sangdun Front Cell Dev Biol Cell and Developmental Biology The diverse applications of genetically modified cells and organisms require more precise and efficient genome-editing tool such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas). The CRISPR/Cas system was originally discovered in bacteria as a part of adaptive-immune system with multiple types. Its engineered versions involve multiple host DNA-repair pathways in order to perform genome editing in host cells. However, it is still challenging to get maximum genome-editing efficiency with fewer or no off-targets. Here, we focused on factors affecting the genome-editing efficiency and precision of CRISPR/Cas system along with its defense-mechanism, orthologues, and applications. Frontiers Media S.A. 2021-11-24 /pmc/articles/PMC8652214/ /pubmed/34901007 http://dx.doi.org/10.3389/fcell.2021.761709 Text en Copyright © 2021 Javaid and Choi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Javaid, Nasir
Choi, Sangdun
CRISPR/Cas System and Factors Affecting Its Precision and Efficiency
title CRISPR/Cas System and Factors Affecting Its Precision and Efficiency
title_full CRISPR/Cas System and Factors Affecting Its Precision and Efficiency
title_fullStr CRISPR/Cas System and Factors Affecting Its Precision and Efficiency
title_full_unstemmed CRISPR/Cas System and Factors Affecting Its Precision and Efficiency
title_short CRISPR/Cas System and Factors Affecting Its Precision and Efficiency
title_sort crispr/cas system and factors affecting its precision and efficiency
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8652214/
https://www.ncbi.nlm.nih.gov/pubmed/34901007
http://dx.doi.org/10.3389/fcell.2021.761709
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