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CRISPR/Cas System and Factors Affecting Its Precision and Efficiency
The diverse applications of genetically modified cells and organisms require more precise and efficient genome-editing tool such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas). The CRISPR/Cas system was originally discovered in bacteria as a part...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8652214/ https://www.ncbi.nlm.nih.gov/pubmed/34901007 http://dx.doi.org/10.3389/fcell.2021.761709 |
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author | Javaid, Nasir Choi, Sangdun |
author_facet | Javaid, Nasir Choi, Sangdun |
author_sort | Javaid, Nasir |
collection | PubMed |
description | The diverse applications of genetically modified cells and organisms require more precise and efficient genome-editing tool such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas). The CRISPR/Cas system was originally discovered in bacteria as a part of adaptive-immune system with multiple types. Its engineered versions involve multiple host DNA-repair pathways in order to perform genome editing in host cells. However, it is still challenging to get maximum genome-editing efficiency with fewer or no off-targets. Here, we focused on factors affecting the genome-editing efficiency and precision of CRISPR/Cas system along with its defense-mechanism, orthologues, and applications. |
format | Online Article Text |
id | pubmed-8652214 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86522142021-12-09 CRISPR/Cas System and Factors Affecting Its Precision and Efficiency Javaid, Nasir Choi, Sangdun Front Cell Dev Biol Cell and Developmental Biology The diverse applications of genetically modified cells and organisms require more precise and efficient genome-editing tool such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas). The CRISPR/Cas system was originally discovered in bacteria as a part of adaptive-immune system with multiple types. Its engineered versions involve multiple host DNA-repair pathways in order to perform genome editing in host cells. However, it is still challenging to get maximum genome-editing efficiency with fewer or no off-targets. Here, we focused on factors affecting the genome-editing efficiency and precision of CRISPR/Cas system along with its defense-mechanism, orthologues, and applications. Frontiers Media S.A. 2021-11-24 /pmc/articles/PMC8652214/ /pubmed/34901007 http://dx.doi.org/10.3389/fcell.2021.761709 Text en Copyright © 2021 Javaid and Choi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Javaid, Nasir Choi, Sangdun CRISPR/Cas System and Factors Affecting Its Precision and Efficiency |
title | CRISPR/Cas System and Factors Affecting Its Precision and Efficiency |
title_full | CRISPR/Cas System and Factors Affecting Its Precision and Efficiency |
title_fullStr | CRISPR/Cas System and Factors Affecting Its Precision and Efficiency |
title_full_unstemmed | CRISPR/Cas System and Factors Affecting Its Precision and Efficiency |
title_short | CRISPR/Cas System and Factors Affecting Its Precision and Efficiency |
title_sort | crispr/cas system and factors affecting its precision and efficiency |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8652214/ https://www.ncbi.nlm.nih.gov/pubmed/34901007 http://dx.doi.org/10.3389/fcell.2021.761709 |
work_keys_str_mv | AT javaidnasir crisprcassystemandfactorsaffectingitsprecisionandefficiency AT choisangdun crisprcassystemandfactorsaffectingitsprecisionandefficiency |