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MicroRNA-425-5p modulates osteoporosis by targeting annexin A2
BACKGROUND: Studies have shown that the decrease of osteogenic differentiation of bone marrow mesenchymal stem cells (MSC) is an important mechanism of osteoporosis. The object of this study was to explore the role and mechanism of microRNA miR-425-5p in the differentiation of MSC. METHODS: The expr...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8653595/ https://www.ncbi.nlm.nih.gov/pubmed/34879879 http://dx.doi.org/10.1186/s12979-021-00256-7 |
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author | Chen, Guanghua Huang, Guizhi Lin, Han Wu, Xinyou Tan, Xiaoyan Chen, Zhoutao |
author_facet | Chen, Guanghua Huang, Guizhi Lin, Han Wu, Xinyou Tan, Xiaoyan Chen, Zhoutao |
author_sort | Chen, Guanghua |
collection | PubMed |
description | BACKGROUND: Studies have shown that the decrease of osteogenic differentiation of bone marrow mesenchymal stem cells (MSC) is an important mechanism of osteoporosis. The object of this study was to explore the role and mechanism of microRNA miR-425-5p in the differentiation of MSC. METHODS: The expression of miR-425-5p in MSC was detected by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Cell proliferation, cell cycle and apoptosis were detected by CCK-8 colorimetry and flow cytometry. The expression of TNF were detected by ELISA. RESULTS: Our data show that MiR-425-5p could modulate TNF-induced cell apoptosis, proliferation, and differentiation. ANXA2 is also the target of miR-425-5p and ANXA2 was involved in TNF-induced MSC cell apoptosis, proliferation, and differentiation. In addition, MiR-425-5p enhanced osteoporosis in mice. CONCLUSION: MiR-425-5p might serve as a potential therapeutic target for the treatment of osteoporosis. |
format | Online Article Text |
id | pubmed-8653595 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-86535952021-12-08 MicroRNA-425-5p modulates osteoporosis by targeting annexin A2 Chen, Guanghua Huang, Guizhi Lin, Han Wu, Xinyou Tan, Xiaoyan Chen, Zhoutao Immun Ageing Research BACKGROUND: Studies have shown that the decrease of osteogenic differentiation of bone marrow mesenchymal stem cells (MSC) is an important mechanism of osteoporosis. The object of this study was to explore the role and mechanism of microRNA miR-425-5p in the differentiation of MSC. METHODS: The expression of miR-425-5p in MSC was detected by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Cell proliferation, cell cycle and apoptosis were detected by CCK-8 colorimetry and flow cytometry. The expression of TNF were detected by ELISA. RESULTS: Our data show that MiR-425-5p could modulate TNF-induced cell apoptosis, proliferation, and differentiation. ANXA2 is also the target of miR-425-5p and ANXA2 was involved in TNF-induced MSC cell apoptosis, proliferation, and differentiation. In addition, MiR-425-5p enhanced osteoporosis in mice. CONCLUSION: MiR-425-5p might serve as a potential therapeutic target for the treatment of osteoporosis. BioMed Central 2021-12-08 /pmc/articles/PMC8653595/ /pubmed/34879879 http://dx.doi.org/10.1186/s12979-021-00256-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Chen, Guanghua Huang, Guizhi Lin, Han Wu, Xinyou Tan, Xiaoyan Chen, Zhoutao MicroRNA-425-5p modulates osteoporosis by targeting annexin A2 |
title | MicroRNA-425-5p modulates osteoporosis by targeting annexin A2 |
title_full | MicroRNA-425-5p modulates osteoporosis by targeting annexin A2 |
title_fullStr | MicroRNA-425-5p modulates osteoporosis by targeting annexin A2 |
title_full_unstemmed | MicroRNA-425-5p modulates osteoporosis by targeting annexin A2 |
title_short | MicroRNA-425-5p modulates osteoporosis by targeting annexin A2 |
title_sort | microrna-425-5p modulates osteoporosis by targeting annexin a2 |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8653595/ https://www.ncbi.nlm.nih.gov/pubmed/34879879 http://dx.doi.org/10.1186/s12979-021-00256-7 |
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