Cas12a target search and cleavage on force-stretched DNA

Using optical tweezers, we investigate target search and cleavage by CRISPR–Cas12a on force-stretched λ-DNA. Cas12a uses fast, one-dimensional hopping to locate its target. Binding and cleavage occur rapidly and specifically at low forces (≤5 pN), with a 1.8 nm rate-limiting conformational change. M...

Descripción completa

Detalles Bibliográficos
Autores principales: Losito, Marialucrezia, Smith, Quentin M., Newton, Matthew D., Cuomo, Maria Emanuela, Rueda, David S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8653695/
https://www.ncbi.nlm.nih.gov/pubmed/34494640
http://dx.doi.org/10.1039/d1cp03408a

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8653695/
https://www.ncbi.nlm.nih.gov/pubmed/34494640
http://dx.doi.org/10.1039/d1cp03408a

Ejemplares similares